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01-12-2014 | Original Contribution

Oleic acid modulates mRNA expression of liver X receptor (LXR) and its target genes ABCA1 and SREBP1c in human neutrophils

Authors: María Edith Reyes-Quiroz, Gonzalo Alba, Javier Saenz, Consuelo Santa-María, Isabel Geniz, Juan Jiménez, Remedios Ramírez, José Martín-Nieto, Elizabeth Pintado, Francisco Sobrino

Published in: European Journal of Nutrition | Issue 8/2014

Abstract

Purpose

Regulation of liver X receptors (LXRs) is essential for cholesterol homeostasis and inflammation. The present study was conducted to determine whether oleic acid (OA) could regulate mRNA expression of LXRα and LXRα-regulated genes and to assess the potential promotion of oxidative stress by OA in neutrophils.

Methods

Human neutrophils were treated with OA at different doses and LXR target gene expression, oxidative stress production, lipid efflux and inflammation state were analyzed.

Results

We describe that mRNA synthesis of both LXRα and ABCA1 (a reverse cholesterol transporter) was induced by OA in human neutrophils. This fatty acid enhanced the effects of LXR ligands on ABCA1 and LXR expression, but it decreased the mRNA levels of sterol regulatory element-binding protein 1c (a transcription factor that regulates the synthesis of triglycerides). Although OA elicited a slight oxidative stress in the short term (15–30 min) in neutrophils, it is unlikely that this is relevant for the modulation of transcription in our experimental conditions, which involve longer incubation time (i.e., 6 h). Of physiological importance is our finding that OA depresses intracellular lipid levels and that markers of inflammation, such as ERK1/2 and p38 mitogen-activated protein kinase phosphorylation, were decreased by OA treatment. In addition, 200 μM OA reduced the migration of human neutrophils, another marker of the inflammatory state. However, OA did not affect lipid peroxidation induced by pro-oxidant agents.

Conclusions

This work presents for the first time evidence that human neutrophils are highly sensitive to OA and provides novel data in support of a protective role of this monounsaturated acid against the activation of neutrophils during inflammation.

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Title: Oleic acid modulates mRNA expression of liver X receptor (LXR) and its target genes ABCA1 and SREBP1c in human neutrophils
Authors: María Edith Reyes-Quiroz
Gonzalo Alba
Javier Saenz
Consuelo Santa-María
Isabel Geniz
Juan Jiménez
Remedios Ramírez
José Martín-Nieto
Elizabeth Pintado
Francisco Sobrino
Publication date: 01-12-2014
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Nutrition / Issue 8/2014
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-014-0677-0

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Oleic acid modulates mRNA expression of liver X receptor (LXR) and its target genes ABCA1 and SREBP1c in human neutrophils

Abstract

Purpose

Methods

Results

Conclusions

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Abstract

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