Skip to main content
Top
Published in: European Journal of Nutrition 1/2013

01-02-2013 | Original Contribution

Adherence to Mediterranean diet and close dietetic supervision increase total dietary antioxidant intake and plasma antioxidant capacity in subjects with abdominal obesity

Authors: Anastasia I. Kolomvotsou, Loukianos S. Rallidis, Konstantinos C. Mountzouris, John Lekakis, Antonis Koutelidakis, Stamatis Efstathiou, Maria Nana-Anastasiou, Antonis Zampelas

Published in: European Journal of Nutrition | Issue 1/2013

Login to get access

Abstract

Purpose

To determine the effect of Mediterranean-type diet and close dietetic supervision on dietary antioxidant intake and plasma total antioxidant capacity (TAC) in patients with abdominal obesity.

Methods

Ninety subjects with abdominal obesity, 46 in intervention group, 44 in control group, participated in a 2-month, randomized, parallel dietary intervention. All participants were counseled on Greek Mediterranean diet. The intervention group was under close dietetic supervision, followed a specific relevant daily and weekly food plan consuming antioxidant-rich foods and food products. Total dietary antioxidant intake was calculated from the volunteers’ food diaries, and plasma TAC using plasma ORAC assay and plasma ferric-reducing antioxidant power (FRAP) assay, both at baseline and at 2 months.

Results

Following the 2-month period, total dietary antioxidant intake was increased in the intervention group compared to the control group (P = 0.000). In addition, increased intake of total fat, due to higher consumption of monounsaturated fatty acids, as well as increased intakes of dietary fiber, vitamin C and alcohol was also observed in the intervention group compared to the control group (P < 0.05). Plasma TAC was increased in the intervention group compared to the control group (P = 0.039) using the ORAC assay, while there was a trend toward a TAC increase (P = 0.077) using the FRAP assay.

Conclusion

Adherence to a Mediterranean-type diet, with emphasis on an increase in foods rich in antioxidants and close dietetic supervision, can increase total dietary antioxidant intake and plasma TAC in patients with abdominal obesity.
Literature
1.
go back to reference Poirier P (2008) Targeting abdominal obesity in cardiology: can we be effective? Can J Cardiol 24(Suppl D):13D–17D Poirier P (2008) Targeting abdominal obesity in cardiology: can we be effective? Can J Cardiol 24(Suppl D):13D–17D
2.
go back to reference Rallidis LS, Lekakis J, Kolomvotsou A, Zampelas A, Vamvakou G, Efstathiou S, Dimitriadis G, Raptis SA, Kremastinos DT (2009) Close adherence to a Mediterranean diet improves endothelial function in subjects with abdominal obesity. Am J Clin Nutr 90(2):263–268CrossRef Rallidis LS, Lekakis J, Kolomvotsou A, Zampelas A, Vamvakou G, Efstathiou S, Dimitriadis G, Raptis SA, Kremastinos DT (2009) Close adherence to a Mediterranean diet improves endothelial function in subjects with abdominal obesity. Am J Clin Nutr 90(2):263–268CrossRef
3.
go back to reference Grundy SM, Brewer HB Jr, Cleeman JI, Smith SC Jr, Lenfant C, American Heart Association, National Heart, Lung, and Blood Institute (2004) Definition of metabolic syndrome: Report of the National Heart, Lung, and Blood Institute/American Heart Association conference on scientific issues related to definition. Circulation 27;109:433–438 Grundy SM, Brewer HB Jr, Cleeman JI, Smith SC Jr, Lenfant C, American Heart Association, National Heart, Lung, and Blood Institute (2004) Definition of metabolic syndrome: Report of the National Heart, Lung, and Blood Institute/American Heart Association conference on scientific issues related to definition. Circulation 27;109:433–438
4.
go back to reference Athyros VG, Bouloukos VI, Pehlivanidis AN, Papageorgiou AA, Dionysopoulou SG, Symeonidis AN, Petridis DI, Kapousouzi MI, Satsoglou EA, Mikhailidis DP, MetS-Greece Collaborative Group (2005) The prevalence of the MS in Greece: the MetS-Greece Multicenter study. Diabetes Obes Metab 7(4):397–405CrossRef Athyros VG, Bouloukos VI, Pehlivanidis AN, Papageorgiou AA, Dionysopoulou SG, Symeonidis AN, Petridis DI, Kapousouzi MI, Satsoglou EA, Mikhailidis DP, MetS-Greece Collaborative Group (2005) The prevalence of the MS in Greece: the MetS-Greece Multicenter study. Diabetes Obes Metab 7(4):397–405CrossRef
5.
go back to reference Taddei S, Ghiadoni L, Salvetti G, Virdis A, Salvetti A (2006) Obesity and endothelial dysfunction. G Ital Cardiol (Rome) 7(11):715–723 Taddei S, Ghiadoni L, Salvetti G, Virdis A, Salvetti A (2006) Obesity and endothelial dysfunction. G Ital Cardiol (Rome) 7(11):715–723
6.
go back to reference Canoy D, Wareham N, Welch A, Bingham S, Luben R, Day N, Khaw KT (2005) Plasma ascorbic acid concentrations and fat distribution in 19 068 British men and women in the European prospective investigation into cancer and nutrition Norfolk cohort study. Am J Clin Nutr 82:1203–1209 Canoy D, Wareham N, Welch A, Bingham S, Luben R, Day N, Khaw KT (2005) Plasma ascorbic acid concentrations and fat distribution in 19 068 British men and women in the European prospective investigation into cancer and nutrition Norfolk cohort study. Am J Clin Nutr 82:1203–1209
7.
go back to reference Lairon D (2007) Review, intervention studies on Mediterranean diet and cardiovascular risk. Mol Nutr Food Res 51:1209–1214 Lairon D (2007) Review, intervention studies on Mediterranean diet and cardiovascular risk. Mol Nutr Food Res 51:1209–1214
8.
go back to reference Pitsavos C, Panagiotakos DB, Chrysohoou C, Papaioannou I, Papadimitriou L, Tousoulis D, Stefanidis C, Toutouzas P (2003) The adoption of Mediterranean diet attenuates the development of acute coronary syndromes in people with the metabolic syndrome. Nutr J 2:1CrossRef Pitsavos C, Panagiotakos DB, Chrysohoou C, Papaioannou I, Papadimitriou L, Tousoulis D, Stefanidis C, Toutouzas P (2003) The adoption of Mediterranean diet attenuates the development of acute coronary syndromes in people with the metabolic syndrome. Nutr J 2:1CrossRef
9.
go back to reference Davis N, Katz S, Wylie-Rosett J (2007) The effect of diet on endothelial function. Cardiol Rev 15:62–66CrossRef Davis N, Katz S, Wylie-Rosett J (2007) The effect of diet on endothelial function. Cardiol Rev 15:62–66CrossRef
10.
go back to reference Hagfors L, Leanderson P, Sköldstam L, Andersson J, Johansson G (2003) Antioxidant intake, plasma antioxidants and oxidative stress in a randomized, controlled, parallel, Mediterranean dietary intervention study on patients with rheumatoid arthritis. Nutr J 2:5CrossRef Hagfors L, Leanderson P, Sköldstam L, Andersson J, Johansson G (2003) Antioxidant intake, plasma antioxidants and oxidative stress in a randomized, controlled, parallel, Mediterranean dietary intervention study on patients with rheumatoid arthritis. Nutr J 2:5CrossRef
11.
go back to reference Pitsavos C, Panagiotakos BD, Tzima N, Chrysohoou C, Economou M, Zampelas A, Stafanidis C (2005) Adherence to the Mediterranean diet is associated with total antioxidant capacity in healthy adults: the Attica study. Am J Clin Nutr 82:694–699 Pitsavos C, Panagiotakos BD, Tzima N, Chrysohoou C, Economou M, Zampelas A, Stafanidis C (2005) Adherence to the Mediterranean diet is associated with total antioxidant capacity in healthy adults: the Attica study. Am J Clin Nutr 82:694–699
12.
go back to reference Razquin C, Martinez JA, Martinez-Gonzalez MA, Mitjavila MT, Estruch R, Marti A (2009) A 3 years follow-up of a Mediterranean diet rich in virgin olive oil is associated with high plasma antioxidant capacity and reduced body weight gain. Eur J Clin Nutr 63(12):1387–1393CrossRef Razquin C, Martinez JA, Martinez-Gonzalez MA, Mitjavila MT, Estruch R, Marti A (2009) A 3 years follow-up of a Mediterranean diet rich in virgin olive oil is associated with high plasma antioxidant capacity and reduced body weight gain. Eur J Clin Nutr 63(12):1387–1393CrossRef
13.
go back to reference Zazpe I, Sanchez-Tainta A, Estruch R, Lamuela-Raventos RM, Schröder H, Salas-Salvado J, Corella D, Fiol M, Gomez-Gracia E, Aros F, Ros E, Ruíz-Gutierrez V, Iglesias P, Conde-Herrera M, Martinez-Gonzalez MA (2008) A large randomized individual and group intervention conducted by registered dietitians increased adherence to Mediterranean-type diets: the PREDIMED study. J Am Diet Assoc 108(7):1134–1144; discussion 1145 Zazpe I, Sanchez-Tainta A, Estruch R, Lamuela-Raventos RM, Schröder H, Salas-Salvado J, Corella D, Fiol M, Gomez-Gracia E, Aros F, Ros E, Ruíz-Gutierrez V, Iglesias P, Conde-Herrera M, Martinez-Gonzalez MA (2008) A large randomized individual and group intervention conducted by registered dietitians increased adherence to Mediterranean-type diets: the PREDIMED study. J Am Diet Assoc 108(7):1134–1144; discussion 1145
15.
go back to reference Cao G, Prior RL (1999) Measurement of oxygen radical absorbance capacity in biological samples. Methods Enzymol 299:50–62CrossRef Cao G, Prior RL (1999) Measurement of oxygen radical absorbance capacity in biological samples. Methods Enzymol 299:50–62CrossRef
16.
go back to reference Benzie IF, Strain JZ (1996) The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem 239:70–76CrossRef Benzie IF, Strain JZ (1996) The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem 239:70–76CrossRef
17.
go back to reference Nigdikar SV, Williams NR, Griffin BA, Howard AN (1998) Consumption of red wine polyphenols reduces the susceptibility of low-density lipoproteins to oxidation in vivo. Am J Clin Nutr 68(2):258–265 Nigdikar SV, Williams NR, Griffin BA, Howard AN (1998) Consumption of red wine polyphenols reduces the susceptibility of low-density lipoproteins to oxidation in vivo. Am J Clin Nutr 68(2):258–265
18.
go back to reference Bemelmans WJ, Broer J, Hulshof KF, Siero FW, May JF, Meyboom-de Jong B (2004) Long-term effects of nutritional group education for persons at high cardiovascular risk. Eur J Public Health 14(3):240–245CrossRef Bemelmans WJ, Broer J, Hulshof KF, Siero FW, May JF, Meyboom-de Jong B (2004) Long-term effects of nutritional group education for persons at high cardiovascular risk. Eur J Public Health 14(3):240–245CrossRef
19.
go back to reference Lapointe A, Goulet J, Couillard C, Lamarche B, Lemieux S (2005) A nutritional intervention promoting the Mediterranean food pattern is associated with a decrease in circulating oxidized LDL particles in healthy women from the Québec City metropolitan area. J Nutr 135(3):410–415 Lapointe A, Goulet J, Couillard C, Lamarche B, Lemieux S (2005) A nutritional intervention promoting the Mediterranean food pattern is associated with a decrease in circulating oxidized LDL particles in healthy women from the Québec City metropolitan area. J Nutr 135(3):410–415
20.
go back to reference Hermsdorff HH, Zulet MÁ, Abete I, Martínez JA (2009) Discriminated benefits of a Mediterranean dietary pattern within a hypocaloric diet program on plasma RBP4 concentrations and other inflammatory markers in obese subjects. Endocrine 36(3):445–451CrossRef Hermsdorff HH, Zulet MÁ, Abete I, Martínez JA (2009) Discriminated benefits of a Mediterranean dietary pattern within a hypocaloric diet program on plasma RBP4 concentrations and other inflammatory markers in obese subjects. Endocrine 36(3):445–451CrossRef
21.
go back to reference Otaolaurruchi E, Fernández-Pachón MS, Gonzalez AG, Troncoso AM, García-Parrilla MC (2007) Repeated red wine consumption and changes on plasma antioxidant capacity and endogenous antioxidants (uric acid and protein thiol groups). J Agric Food Chem 55(23):9713–9718CrossRef Otaolaurruchi E, Fernández-Pachón MS, Gonzalez AG, Troncoso AM, García-Parrilla MC (2007) Repeated red wine consumption and changes on plasma antioxidant capacity and endogenous antioxidants (uric acid and protein thiol groups). J Agric Food Chem 55(23):9713–9718CrossRef
22.
go back to reference Pellegrini N, Salvatore S, Valtueña S, Bedogni G, Porrini M, Pala V, Del Rio D, Sieri S, Miglio C, Krogh V, Zavaroni I, Brighenti F (2007) Development and validation of a food frequency questionnaire for the assessment of dietary total antioxidant capacity. J Nutr 137(1):93–98 Pellegrini N, Salvatore S, Valtueña S, Bedogni G, Porrini M, Pala V, Del Rio D, Sieri S, Miglio C, Krogh V, Zavaroni I, Brighenti F (2007) Development and validation of a food frequency questionnaire for the assessment of dietary total antioxidant capacity. J Nutr 137(1):93–98
23.
go back to reference Cao G, Russell RM, Lischner N, Prior RL (1998) Serum antioxidant capacity is increased by consumption of strawberries, spinach, red wine or vitamin C in elderly women. J Nutr 128:2383–2390 Cao G, Russell RM, Lischner N, Prior RL (1998) Serum antioxidant capacity is increased by consumption of strawberries, spinach, red wine or vitamin C in elderly women. J Nutr 128:2383–2390
24.
go back to reference Djuric Z, Ren J, Blythe J, VanLoon G, Sen A (2009) A Mediterranean dietary intervention in healthy American women changes plasma carotenoids and fatty acids in distinct clusters. Nutr Res 29(3):156–163CrossRef Djuric Z, Ren J, Blythe J, VanLoon G, Sen A (2009) A Mediterranean dietary intervention in healthy American women changes plasma carotenoids and fatty acids in distinct clusters. Nutr Res 29(3):156–163CrossRef
25.
go back to reference Kawashima A, Madarame T, Koike H, Komatsu Y, Wise JA (2007) Four week supplementation with mixed fruit and vegetable juice concentrates increased protective serum antioxidants and folate and decreased plasma homocysteine in Japanese subjects. Asia Pac J Clin Nutr 16(3):411–421 Kawashima A, Madarame T, Koike H, Komatsu Y, Wise JA (2007) Four week supplementation with mixed fruit and vegetable juice concentrates increased protective serum antioxidants and folate and decreased plasma homocysteine in Japanese subjects. Asia Pac J Clin Nutr 16(3):411–421
26.
go back to reference Strain JJ, Elwood PC, Davis A, Kennedy O, Coulter J, Fehily A, Mulholland CW, Robson PJ, Thurnham DI (2000) Frequency of fruit and vegetable consumption and blood antioxidants in the Caerphilly cohort of older men. Eur J Clin Nutr 54(11):828–833CrossRef Strain JJ, Elwood PC, Davis A, Kennedy O, Coulter J, Fehily A, Mulholland CW, Robson PJ, Thurnham DI (2000) Frequency of fruit and vegetable consumption and blood antioxidants in the Caerphilly cohort of older men. Eur J Clin Nutr 54(11):828–833CrossRef
27.
go back to reference Zino S, Skeaff M, Williams S, Mann J (1997) Randomized controlled trial of effect of fruit and vegetable consumption on plasma concentrations of lipids and antioxidants. BMJ 314(7097):1787–1791CrossRef Zino S, Skeaff M, Williams S, Mann J (1997) Randomized controlled trial of effect of fruit and vegetable consumption on plasma concentrations of lipids and antioxidants. BMJ 314(7097):1787–1791CrossRef
28.
go back to reference Leighton F, Cuevas A, Guasch V, Pérez DD, Strobel P, San Martín A, Urzua U, Díez MS, Foncea R, Castillo O, Mizón C, Espinoza MA, Urquiaga I, Rozowski J, Maiz A, Germain A (1999) Plasma polyphenols and antioxidants, oxidative DNA damage and endothelial function in a diet and wine intervention study in humans. Drugs Exp Clin Res 25(2–3):133–141 Leighton F, Cuevas A, Guasch V, Pérez DD, Strobel P, San Martín A, Urzua U, Díez MS, Foncea R, Castillo O, Mizón C, Espinoza MA, Urquiaga I, Rozowski J, Maiz A, Germain A (1999) Plasma polyphenols and antioxidants, oxidative DNA damage and endothelial function in a diet and wine intervention study in humans. Drugs Exp Clin Res 25(2–3):133–141
29.
go back to reference Puchau B, Zulet MA, de Echávarri AG, Hermsdorff HH, Martínez JA (2010) Dietary total antioxidant capacity is negatively associated with some metabolic syndrome features in healthy young adults. Nutrition 26(5):534–541CrossRef Puchau B, Zulet MA, de Echávarri AG, Hermsdorff HH, Martínez JA (2010) Dietary total antioxidant capacity is negatively associated with some metabolic syndrome features in healthy young adults. Nutrition 26(5):534–541CrossRef
30.
go back to reference Rautiainen S, Serafini M, Morgenstern R, Prior RL, Wolk A (2008) The validity and reproducibility of food-frequency questionnaire-based total antioxidant capacity estimates in Swedish women. Am J Clin Nutr 87(5):1247–1253 Rautiainen S, Serafini M, Morgenstern R, Prior RL, Wolk A (2008) The validity and reproducibility of food-frequency questionnaire-based total antioxidant capacity estimates in Swedish women. Am J Clin Nutr 87(5):1247–1253
31.
go back to reference Ca G, Prior R (1998) Comparison of different analytical methods for assessing total antioxidant capacity of human serum. Clin Chem 44(6):1309–1315 Ca G, Prior R (1998) Comparison of different analytical methods for assessing total antioxidant capacity of human serum. Clin Chem 44(6):1309–1315
32.
go back to reference McKay DL, Perrone G, Rasmussen H, Dallal G, Hartman W, Cao G, Prior RL, Roubenoff R, Blumberg JB (2000) The effects of a multivitamin/mineral supplement on micronutrient status, antioxidant capacity and cytokine production in healthy older adults consuming a fortified diet. J Am Coll Nutr 19(5):613–621 McKay DL, Perrone G, Rasmussen H, Dallal G, Hartman W, Cao G, Prior RL, Roubenoff R, Blumberg JB (2000) The effects of a multivitamin/mineral supplement on micronutrient status, antioxidant capacity and cytokine production in healthy older adults consuming a fortified diet. J Am Coll Nutr 19(5):613–621
33.
go back to reference Brighenti F, Valtueña S, Pellegrini N, Ardigò D, Del Rio D, Salvatore S, Piatti P, Serafini M, Zavaroni I (2005) Total antioxidant capacity of the diet is inversely and independently related to plasma concentration of high-sensitivity C-reactive protein in adult Italian subjects. Br J Nutr 93(5):619–625CrossRef Brighenti F, Valtueña S, Pellegrini N, Ardigò D, Del Rio D, Salvatore S, Piatti P, Serafini M, Zavaroni I (2005) Total antioxidant capacity of the diet is inversely and independently related to plasma concentration of high-sensitivity C-reactive protein in adult Italian subjects. Br J Nutr 93(5):619–625CrossRef
34.
go back to reference Brooks GC, Blaha MJ, Blumenthal RS (2010) Relation of C-reactive protein to abdominal adiposity. Am J Cardiol 106(1):56–61CrossRef Brooks GC, Blaha MJ, Blumenthal RS (2010) Relation of C-reactive protein to abdominal adiposity. Am J Cardiol 106(1):56–61CrossRef
35.
go back to reference Brehm BJ, Spang SE, Lattin BL, Seeley RJ, Daniels SR, D’Alessio DA (2005) The role of energy expenditure in the differential weight loss in obese women on low-fat and low-carbohydrate diets. J Clin Endocrinol Metab 90(3):1475–1482CrossRef Brehm BJ, Spang SE, Lattin BL, Seeley RJ, Daniels SR, D’Alessio DA (2005) The role of energy expenditure in the differential weight loss in obese women on low-fat and low-carbohydrate diets. J Clin Endocrinol Metab 90(3):1475–1482CrossRef
Metadata
Title
Adherence to Mediterranean diet and close dietetic supervision increase total dietary antioxidant intake and plasma antioxidant capacity in subjects with abdominal obesity
Authors
Anastasia I. Kolomvotsou
Loukianos S. Rallidis
Konstantinos C. Mountzouris
John Lekakis
Antonis Koutelidakis
Stamatis Efstathiou
Maria Nana-Anastasiou
Antonis Zampelas
Publication date
01-02-2013
Publisher
Springer-Verlag
Published in
European Journal of Nutrition / Issue 1/2013
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI
https://doi.org/10.1007/s00394-011-0283-3

Other articles of this Issue 1/2013

European Journal of Nutrition 1/2013 Go to the issue