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01-02-2007 | ORIGINAL CONTRIBUTION

Inhibition of phospholipase A₂ activity by conjugated linoleic acids in human macrophages

Authors: Ewa Stachowska, Violetta Dziedziejko, Krzysztof Safranow, Izabela Gutowska, Grażyna Adler, Andrzej Ciechanowicz, Bogusław Machaliński, Dariusz Chlubek

Published in: European Journal of Nutrition | Issue 1/2007

Abstract

The objective of this study was to assess the effect of conjugated linoleic acid isomers (CLAs) on the expression and activity of phospholipases A₂ (PLA₂) in human macrophages.

Macrophages were incubated with 30 µM cis-9, trans-11 and trans-10, cis-12 CLAs for 48 h. After incubation, the total activity of phospholipases as well as the expression of mRNA for cytosolic (cPLA₂) and secretory (sPLA₂) phospholipases and activity of sPLA₂ were measured.

Both CLA isomers reduced the total activity of PLA₂ (by 30.2%, P < 0.01 for cis-9, trans-11 CLA and by 30%, P < 0.001 for trans-10, cis-12 CLA). Trans-10, cis-12 CLA isomer downregulated the expression of mRNA of sPLA₂ and decreased the enzymatic activity of this enzyme (by 23%, P = 0.02) in macrophages.

Conjugated linoleic acid isomers can significantly reduce the activity of PLA₂ in macrophages and downregulate sPLA₂ expression. The consequence of this effect may be reduction of releasing the arachidonic acid (AA) from the cellular membranes of macrophages.

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Title: Inhibition of phospholipase A2 activity by conjugated linoleic acids in human macrophages
Authors: Ewa Stachowska
Violetta Dziedziejko
Krzysztof Safranow
Izabela Gutowska
Grażyna Adler
Andrzej Ciechanowicz
Bogusław Machaliński
Dariusz Chlubek
Publication date: 01-02-2007
Publisher: Steinkopff-Verlag
Published in: European Journal of Nutrition / Issue 1/2007
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-006-0626-7

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Inhibition of phospholipase A₂ activity by conjugated linoleic acids in human macrophages

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