Top

Pediatric Surgery International

Published in:

01-01-2012 | Original Article

Topical application of basic fibroblast growth factor reduces esophageal stricture and esophageal neural damage after sodium hydroxide-induced esophagitis in rats

Authors: Yuichi Okata, Chieko Hisamatsu, Eiji Nishijima, Yutaka Okita

Published in: Pediatric Surgery International | Issue 1/2012

Abstract

Purpose

To investigate the effect of topical application of basic fibroblast growth factor (bFGF) after caustic esophageal injury in rats.

Methods

Thirty-six male rats were randomly divided into three groups. Corrosive esophageal injury was produced by internal application of 30% sodium hydroxide (NaOH) solution to the distal esophagus. Group A rats were uninjured. Group B rats were injured and untreated. Group C rats were injured and received topical bFGF (10 μg/ml). Surviving rats were killed at 28 days. The survival rate, body weight gain, symptoms and histopathological changes that included tissue damage score, ratio of esophageal luminal area/total esophageal area (LA/TA) and the proportion of a neural marker PGP 9.5-positive area were assessed.

Results

The survival rate and the prevalence of symptoms were not significantly different between Groups B and C. Although the tissue damage score did not differ in Groups B and C, LA/TA was significantly higher in Group C than in Group B. The proportion of the PGP 9.5-positive area was significantly lower in Groups B and C than in Group A; however, it was higher in Group C than in Group B.

Conclusion

Topical application of bFGF was effective in preventing stricture after NaOH-induced esophagitis.

Kay M, Wyllie R (2009) Caustic ingestions in children. Curr Opin Pediatr 21:651–654PubMedCrossRef

Riffat F, Cheng A (2009) Pediatric caustic ingestion: 50 consecutive cases and a review of the literature. Dis Esophagus 22:89–94PubMedCrossRef

Nuutinen M, Uhari M, Karvali T, Kouvalainen K (1994) Consequences of caustic ingestions in children. Acta Paediatr 83:1200–1205PubMed

Kirsh MM, Ritter F (1976) Caustic ingestion and subsequent damage to the oropharyngeal and digestive passages. Ann Thorac Surg 21:74–82PubMedCrossRef

Spitz L, Lakhoo K (1993) Caustic ingestion. Arch Dis Child 68:157–158PubMedCrossRef

Anderson KD, Rouse TM, Randolph JG (1990) A controlled trial of corticosteroids in children with corrosive injury of the esophagus. N Engl J Med 323:637–640PubMedCrossRef

Mamede RC, De Mello Filho FV (2002) Treatment of caustic ingestion: an analysis of 239 cases. Dis Esophagus 15:210–213PubMedCrossRef

Tiryaki T, Livanelioglu Z, Atayurt H (2005) Early bougienage for relief of stricture formation following caustic esophageal burns. Pediatr Surg Int 21:78–80PubMedCrossRef

Reyes HM, Hill JL (1976) Modification of the experimental stent technique for esophageal burns. J Surg Res 20:65–70PubMedCrossRef

10.

Zhou JH, Jiang YG, Wang RW, Fan SZ, Gong TQ, Tan QY, Ma Z, Zhao YP, Deng B (2008) Prevention of stricture development after corrosive esophageal burn with a modified esophageal stent in dogs. J Thorac Cardiovasc Surg 136:1336–1342, 1342, e1331–e1337

11.

Zhou JH, Jiang YG, Wang RW, Lin YD, Gong TQ, Zhao YP, Ma Z, Tan QY (2005) Management of corrosive esophageal burns in 149 cases. J Thorac Cardiovasc Surg 130:449–455PubMedCrossRef

12.

Guven A, Demirbag S, Uysal B, Topal T, Erdogan E, Korkmaz A, Ozturk H (2008) Effect of 3-amino benzamide, a poly(adenosine diphosphate-ribose) polymerase inhibitor, in experimental caustic esophageal burn. J Pediatr Surg 43:1474–1479PubMedCrossRef

13.

Yukselen V, Vardar E, Yukselen O, Oruc N, Yenisey C, Karaoglu A, Ozutemiz O (2008) Infliximab in experimental alkali burns of the oesophagus in the rat. Acta Gastroenterol Belg 71:21–26PubMed

14.

Turkyilmaz Z, Sonmez K, Demirtola A, Karabulut R, Poyraz A, Gulen S, Dincer S, Basaklar AC, Kale N (2005) Mitomycin C prevents strictures in caustic esophageal burns in rats. J Surg Res 123:182–187PubMedCrossRef

15.

Bakan V, Garipardic M, Okumus M, Ciralik H, Atli Y, Ozbag D, Tolun FI (2010) The protective effect of erythropoietin on the acute phase of corrosive esophageal burns in a rat model. Pediatr Surg Int 26:195–201PubMedCrossRef

16.

Ceylan H, Yapici S, Tutar E, Ceylan NO, Tarakcioglu M, Demiryurek AT (2010) Protective effects of dexpanthenol and Y-27632 on stricture formation in a rat model of caustic esophageal injury. J Surg Res

17.

Herek O, Karabul M, Yenisey C, Erkus M (2010) Protective effects of ibuprofen against caustic esophageal burn injury in rats. Pediatr Surg Int 26:721–727PubMedCrossRef

18.

Uguralp S, Irsi C, Aksoy T, Karabulut AB, Kirimlioglu H, Mizrak B (2008) Resveratrol attenuates inflammation and stricture formation in experimental caustic esophageal burns. Pediatr Surg Int 24:425–430PubMedCrossRef

19.

Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M (2008) Growth factors and cytokines in wound healing. Wound Repair Regen 16:585–601PubMedCrossRef

20.

Fu X, Shen Z, Chen Y, Xie J, Guo Z, Zhang M, Sheng Z (1998) Randomised placebo-controlled trial of use of topical recombinant bovine basic fibroblast growth factor for second-degree burns. Lancet 352:1661–1664PubMedCrossRef

21.

Akita S, Akino K, Imaizumi T, Hirano A (2008) Basic fibroblast growth factor accelerates and improves second-degree burn wound healing. Wound Repair Regen 16:635–641PubMedCrossRef

22.

Robson MC, Phillips LG, Lawrence WT, Bishop JB, Youngerman JS, Hayward PG, Broemeling LD, Heggers JP (1992) The safety and effect of topically applied recombinant basic fibroblast growth factor on the healing of chronic pressure sores. Ann Surg 216:401–406 (discussion 406–408)PubMedCrossRef

23.

National, Research, Council, Institute, of, Laboratory, Animal, Research, Commission, on, Life, Sciences (eds) (1996) Guide for the care and use of laboratory animals. National Academy Press, Washington, DC

24.

Okata Y, Hisamatsu C, Hasegawa T, Nishijima E, Okita Y (2011) Development of a model of benign esophageal stricture in rats: the optimal concentration of sodium hydroxide for stricture formation. Pediatr Surg Int 27:73–80PubMedCrossRef

25.

Vardar E, Vardar R, Yukselen V, Makay O, Erkan N, Bayol U, Ozutemiz O (2009) Image-based assessment of esophageal stricture in experimental corrosive esophagitis in animals: an objective, adjunct diagnostic tool. Turk J Gastroenterol 20:3–8PubMed

26.

Pederiva F, Lopez RA, Martinez L, Tovar JA (2009) Tracheal innervation is abnormal in rats with experimental congenital diaphragmatic hernia. J Pediatr Surg 44:1159–1164PubMedCrossRef

27.

Pederiva F, Aras Lopez R, Martinez L, Tovar JA (2008) Abnormal development of tracheal innervation in rats with experimental diaphragmatic hernia. Pediatr Surg Int 24:1341–1346PubMedCrossRef

28.

Oh JT, Han AR, Yang WI, Han SJ, Choi SH, Hwang EH (2002) Morphometric evaluation of PGP9.5 and NCAM expressing nerve fibers in colonic muscle of patients with Hirschsprung’s disease. Yonsei Med J 43:31–36PubMed

29.

McGee GS, Davidson JM, Buckley A, Sommer A, Woodward SC, Aquino AM, Barbour R, Demetriou AA (1988) Recombinant basic fibroblast growth factor accelerates wound healing. J Surg Res 45:145–153PubMedCrossRef

30.

Ono I, Akasaka Y, Kikuchi R, Sakemoto A, Kamiya T, Yamashita T, Jimbow K (2007) Basic fibroblast growth factor reduces scar formation in acute incisional wounds. Wound Repair Regen 15:617–623PubMedCrossRef

31.

Akasaka Y, Ono I, Kamiya T, Ishikawa Y, Kinoshita T, Ishiguro S, Yokoo T, Imaizumi R, Inomata N, Fujita K, Akishima-Fukasawa Y, Uzuki M, Ito K, Ishii T (2010) The mechanisms underlying fibroblast apoptosis regulated by growth factors during wound healing. J Pathol 221:285–299PubMedCrossRef

32.

Akasaka Y, Ono I, Tominaga A, Ishikawa Y, Ito K, Suzuki T, Imaizumi R, Ishiguro S, Jimbow K, Ishii T (2007) Basic fibroblast growth factor in an artificial dermis promotes apoptosis and inhibits expression of alpha-smooth muscle actin, leading to reduction of wound contraction. Wound Repair Regen 15:378–389PubMedCrossRef

33.

Akasaka Y, Ono I, Yamashita T, Jimbow K, Ishii T (2004) Basic fibroblast growth factor promotes apoptosis and suppresses granulation tissue formation in acute incisional wounds. J Pathol 203:710–720PubMedCrossRef

34.

Singaram C, Sweet MA, Gaumnitz EA, Cameron AJ, Camilleri M (1995) Peptidergic and nitrinergic denervation in congenital esophageal stenosis. Gastroenterology 109:275–281PubMedCrossRef

35.

Filogamo G, Cracco C (1995) Models of neuronal plasticity and repair in the enteric nervous system: a review. Ital J Anat Embryol 100(Suppl 1):185–195PubMed

36.

Okazaki T, Yamataka A, Fujiwara T, Nishiye H, Fujimoto T, Miyano T (1994) Abnormal distribution of nerve terminals in infantile hypertrophic pyloric stenosis. J Pediatr Surg 29:655–658PubMedCrossRef

37.

Marlow SL, Blennerhassett MG (2006) Deficient innervation characterizes intestinal strictures in a rat model of colitis. Exp Mol Pathol 80:54–66PubMedCrossRef

38.

Ono I, Tateshita T, Inoue M (1999) Effects of a collagen matrix containing basic fibroblast growth factor on wound contraction. J Biomed Mater Res 48:621–630PubMedCrossRef

Title: Topical application of basic fibroblast growth factor reduces esophageal stricture and esophageal neural damage after sodium hydroxide-induced esophagitis in rats
Authors: Yuichi Okata
Chieko Hisamatsu
Eiji Nishijima
Yutaka Okita
Publication date: 01-01-2012
Publisher: Springer-Verlag
Published in: Pediatric Surgery International / Issue 1/2012
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-011-3007-0

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Topical application of basic fibroblast growth factor reduces esophageal stricture and esophageal neural damage after sodium hydroxide-induced esophagitis in rats

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 1/2012

Intraoperative bronchoscopy for bronchial carcinoid parenchymal-sparing resection: a pediatric case report

Percutaneous ultrasound-guided thrombin injection of a post-traumatic pancreatic pseudoaneurysm in a pediatric patient

Late-onset vital complication after the Nuss procedure for pectus excavatum

A comparison of clinical protocols for assessing postoperative fecal continence in anorectal malformation

Surgical intervention strategies for pediatric ovarian tumors: experience with 60 cases at one institution

Long-term survival with cloacal dysgenesis sequence