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Pediatric Surgery International

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01-01-2011 | Original Article

Development of a model of benign esophageal stricture in rats: the optimal concentration of sodium hydroxide for stricture formation

Authors: Yuichi Okata, Chieko Hisamatsu, Tomomi Hasegawa, Eiji Nishijima, Yutaka Okita

Published in: Pediatric Surgery International | Issue 1/2011

Abstract

Purpose

To investigate the optimal concentration of sodium hydroxide (NaOH) on esophageal stricture formation in rats to establish an animal model of benign esophageal stricture (BES).

Methods

Corrosive esophageal burn was produced by internal application of different concentrations of NaOH to the distal esophagus in rats. As much as 66 male rats were randomly divided into eight groups: Group A (control, n = 6), Group B (sham-operated group, n = 6), Group C (5% NaOH, n = 8), Group D (10% NaOH, n = 8), Group E (20% NaOH, n = 8), Group F (30% NaOH, n = 10), Group G (40% NaOH, n = 14), and Group H (50% NaOH, n = 6). Surviving rats were killed at 28 days. The survival rate, body weight gain, symptoms, and histopathological changes were assessed.

Results

The mortality rate was high in Groups G and H (73 and 67%). The prevalence of symptoms of BES was 43% in Groups D and E, 50% in Group F, 75% in Group G, and 100% in Group H. Statistically significant stricture formation of the esophagus was observed in Groups F and G. The degree of tissue damage was significantly higher in Groups E, F, and G.

Conclusion

A high concentration of NaOH of 30% was required to establish a survivable BES model in rats.

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Title: Development of a model of benign esophageal stricture in rats: the optimal concentration of sodium hydroxide for stricture formation
Authors: Yuichi Okata
Chieko Hisamatsu
Tomomi Hasegawa
Eiji Nishijima
Yutaka Okita
Publication date: 01-01-2011
Publisher: Springer-Verlag
Published in: Pediatric Surgery International / Issue 1/2011
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-010-2711-5

At a glance: The STEP trials

Springer Medicine

Development of a model of benign esophageal stricture in rats: the optimal concentration of sodium hydroxide for stricture formation

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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