Top

Pediatric Surgery International

Published in:

01-07-2010 | Original Article

Protective effects of ibuprofen against caustic esophageal burn injury in rats

Authors: Özkan Herek, Murat Karabul, Çiğdem Yenisey, Muhan Erkuş

Published in: Pediatric Surgery International | Issue 7/2010

Abstract

Purpose

The aim of this study was to investigate the efficacy of ibuprofen on the healing of esophagus and the prevention of stricture development after esophageal caustic injuries in rats.

Materials and methods

Rats were divided into three groups as: group 1(sham), group 2(esophageal burn injury), group 3(injury + ibuprofen). In groups 2 and 3, a standard esophageal burn injury was created by applying 10% NaOH solution to distal esophagus of about 3 cm. To rats in the sham group, isotonic solution was given instead of NaOH. Ibuprofen (90 mg/kg/day) was given via oral route to group 3 rats. Normal saline as placebo was given via the same route to rats in groups 1 and 2. 28 days later, all the live rats were killed. The distal esophageal segments of all rats were removed and divided into two equal parts for biochemical and histopathologic examination. In the tissue samples, biochemically hydroxyproline and histopathologically collagen content and stenosis indices were evaluated for efficacy of treatment.

Results

The hydroxyproline level (μg/mg wet tissue) in the groups was 1.54 ± 0.08, 4.82 ± 0.60, and 3.28 ± 0.27, respectively. The hydroxyproline level increased significantly in group 2 compared with group 1 (P < 0.01). Although the hydroxyproline level was significantly increased in group 3 compared with group 1, it decreased significantly in group 3 compared with group 2 (P < 0.05) by treatment of ibuprofen. In group 3, the collagen content score (1.50 ± 0.26) was significantly lower than in group 2 (2.62 ± 0.37) (P < 0.05). The stenosis index was found as 0.37 ± 0.02 in group 1, 0.84 ± 0.02 in group 2, and 0.67 ± 0.03 in group 3. The stenosis index in group 2 was significantly higher than group 1 and group 3 (P < 0.01). Although the stenosis index was significantly higher than in group 1, a significant decrease in stenosis index was found in group 3 compared with group 2, by ibuprofen treatment (P < 0.01).

Conclusion

Based on these results, we concluded that the treatment with ibuprofen in acute phase esophageal burn injury has beneficial effects on healing of esophagus and may decrease the stricture formation. For these reasons, ibuprofen may effectively be used in the acute phase treatment of caustic esophagus injury and after esophageal dilatation procedures.

Hamza AF, Abdelhay S, Sherif H, Hasan T, Soliman H, Kabesh A, Bassiouny I, Bahnassy AF (2003) Caustic esophageal strictures in children: 30 years’ experience. J Pediatr Surg 38:828–833CrossRefPubMed

Baskın D, Urgancı N, Abbasoğlu L, Alkım C, Yalçın M, Karadağ Ç, Sever N (2004) A standardized protocol for the acute management of corrosive ingestion in children. Pediatr Surg Int 20:824–828CrossRefPubMed

Han Y, Cheng QS, Li XF, Wang XP (2004) Surgical management of esophageal strictures after caustic burns: a 30 years experience. World J Gastroenterol 10:2846–2849PubMed

Jong AL, Macdonald R, Ein S, Forte V, Turner A (2001) Corrosive esophagitis in children: a 30-year review. Int J Pediatr Otorhinolaryngol 57:203–211CrossRefPubMed

Broto J, Asensio M, Jorro CS, Marhuenda C, JMG Acosta D, Ochoa JB (1999) Conservative treatment of caustic esophageal injuries in children : 20 years of experience. Pediatr Surg Int 15:323–325CrossRefPubMed

Anderson KD, Rouse TM, Randolph JG (1990) A controlled trial of corticosteroids in children with corrosive injury of the esophagus. N Engl J Med 323:637–640PubMedCrossRef

Ashcraft KW (2005) The esophagus. In: Ashcraft KW, Holcomb GW III, Murphy JP (eds) Pediatric Surgery, 4th edn. Elsevier Saunders, Philadelphia, pp 330–351

Bautista A, Varela R, Villanueva A, Estevez E, Tojo R, Cadranel S (1996) Effects of prednisolone anf dexamethasone in children with alkali burns of the oesophagus. Eur J Pediatr 6:198–203CrossRef

Tanyel FC, Büyükpamukcu N, Hiçsönmez A (1988) The place of steroids, antibiotics and early bougienage combination in the treatment of cautic esophageal burns in childhood. Turk J Pediatr 30:253–257

10.

Ulman İ, Mutaf O (1998) A critique of systemic steroids in the management of caustic esophageal burns in children. Eur J Pediatr Surg 6:71–74CrossRef

11.

Günel E, Çağlayan F, Çağlayan O, Canbilen A, Tosun M (2002) Effect of antioxidant therapy on collagen synthesis in corrosive esophageal burns. Pediatr Surg Int 18:24–27CrossRefPubMed

12.

Topaloglu B, Bicakci U, Tander B, Ariturk E, Kilicoglu-Aydin B, Aydin O, Rizalar R, Ayyildiz SH, Bernay F (2008) Biochemical and histopathologic effects of omeprazole and vitamin E in rats with corrosive esophageal burns. Pediatr Surg Int 24:555–560CrossRefPubMed

13.

Larios-Arceo F, Ortiz GG, Huerta M, Leal-Cortés C, Saldaña JA, Bitzer-Quintero OK, Rodríguez-Reynoso S (2008) Protective effects of melatonin against caustic esophageal burn injury in rats. J Pineal Res 45:219–223CrossRefPubMed

14.

Ocakci A, Kanter M, Cabuk M, Buyukbas S (2006) Role of caffeic acid phenetyl ester, an active component of propolis, against NaOH-induced esophageal burns in rats. Int J Pediatr Otorhinolaryngol 70:1731–1739CrossRefPubMed

15.

Koltuksuz U, Mutuş HM, Kutlu R, Özyurt H, Çetin S, Karaman A, Gürbüz N, Akyol Ö, Aydın NE (2001) Effects of caffeic acid phenetyl ester and epidermal growth factor on the development of caustic esophageal stricture in rats. J Pediatr Surg 36:1504–1509CrossRefPubMed

16.

Makay O, Yukselen V, Vardar E, Yenisey C, Bicakci T, Ersin S, Ozutemiz O (2007) Role of allopurinol on oxidative stress in caustic burn: cure for stricture? Pediatr Surg Int 23:1105–1112CrossRefPubMed

17.

Yukselen V, Karaoglu AO, Ozutemiz O, Yenisey C, Tuncyurek M (2004) Ketotifen ameliorates development of fibrosis in alkali burns of the esophagus. Pediatr Surg Int 20:429–433CrossRefPubMed

18.

Türkyılmaz Z, Sönmez K, Demirtola A, Karabulut R, Poyraz A, Gülen Ş, Dinçer S, Başaklar AC, Kale N (2005) Mitomycin C prevents strictures in caustic esophageal burns in rats. J Surg Res 123:182–187CrossRefPubMed

19.

Bingöl-Koloğlu M, Tanyel FC, Müftüoğlu S, Renda N, Çakar N, Büyükpamukçu N, Hiçsönmez A (1999) The preventive effect of heparin on stricture formation after caustic esophageal burns. J Pediatr Surg 34:291–294CrossRefPubMed

20.

Liu AJ, Richardson MA (1985) Effects of N-acetylcysteine on experimentally induced esophageal lye injury. Ann Otol Rhinol Laryngol 95:477–482

21.

Ekingen G, Özden M, Sözübir S, Maral H, Müezzinoğlu B, Kahraman H, Güvenç BH (2005) Effect of the prostacyclin derivate iloprost in experimental caustic esophageal burn. Pediatr Surg Int 21:441–444CrossRefPubMed

22.

Yukselen V, Vardar E, Yukselen O, Karaoglu AO, Yenisey C, Ozutemiz O (2006) Colchicine in experimental alkaline burns of the rat esophagus: an old drug, a new indication? Pediatr Surg Int 22:363–368CrossRefPubMed

23.

Ocakci A, Coskun O, Tumkaya L, Kanter M, Gurel A, Hostuner M, Uzun L (2006) Beneficial effects of ebselen on corrosive esophageal burns of rats. Int J Pediatr Otorhinolaryngol 70:45–52CrossRefPubMed

24.

Yukselen V, Karaoglu AO, Yenisey C, Tuncyurek M, Ozutemiz O (2005) Trimetazidine reduces the degree of fibrosis in alkali burns of the esophagus. J Pediatr Surg 40:505–509CrossRefPubMed

25.

Kiyan G, Aktas S, Ozel K, İsbilen E, Kotiloglu E, Dagli TE (2004) Effects of hyperbaric oxygen therapy on caustic esophageal injury in rats. J Pediatr Surg 39:1188–1193CrossRefPubMed

26.

Demirbilek S, Bernay F, Rizalar R, Bariş S, Gürses N (1994) Effects of estrodiol and progesterone on the synthesis of collagen in corrosive esophageal burns in rats. J Pediatr Surg 29:1425–1428CrossRefPubMed

27.

Temir ZG, Karkıner A, Karaca İ, Ortaç R, Özdamar A (2005) The effectiveness of sucralfate against stricture formation in experimental corrosive esophageal burns. Surg Today 35:617–622CrossRefPubMed

28.

Pul M, Yilmaz N, Deger O, Gürses N (1990) Indomethacin for prevention of stricture formation due to alkali-induced corrosive esophageal burns in the rat. Pediatr Surg Int 5:416–417CrossRef

29.

Lesko SM, Mitchell AA (1995) An assessment of safety of pediatric ibuprofen. JAMA 273:929–933CrossRefPubMed

30.

Rainsford KD (2009) Ibuprofen: pharmacology, efficacy and safety. Inflammopharmacol 17:275–342CrossRef

31.

Gehanno P, Guedon C (1981) Inhibition of experimental esophageal lye strictures by penicillamin. Arch Otolaryngol 107:145–147PubMed

32.

Reddy GK, Enwemeka CS (1996) A simplified method for the analysis of hydroxyproline in biological tissues. Clin Biochem 29:225–229CrossRefPubMed

33.

Berthet B, di Costanzo J, Arnaud C, Choux R, Assadourian R (1994) Influence of epidermal growth factor and interferon γ on healing of oesophageal corrosive burns in the rat. Br J Surg 81:395–398CrossRefPubMed

34.

Günel E, Çağlayan F, Çağlayan O, Akıllıoğlu İ (1999) Reactive oxygen radical levels in caustic esophageal burns. J Pediatr Surg 34:405–407CrossRefPubMed

35.

Ozel SK, Dagli TE, Yuksel M, Kiyan G, Kotiloglu E (2004) The roles of free oxygen radicals, nitric oxide, and endothelin in caustic injury of rat esophagus. J Pediatr Surg 39:1381–1385CrossRefPubMed

36.

De Palma C, Di Paola R, Perrotta C, Mazzon E, Cattaneo D, Emilio T, Cuzzocrea S, Clementi E (2009) Ibuprofen-arginine generates nitric oxide and has enhanced anti-inflammatory effects. Pharmacol Res 60:221–228CrossRefPubMed

37.

Costa D, Moutinho L, Lima JLFC, Fernandes E (2006) Antioxidant activity and inhibition of human neutrophil oxidative burst mediated by arylpropionic acid non-steroidal anti-inflammatory drugs. Biol Pharm Bull 29:1659–1670CrossRefPubMed

38.

Dokmeci D, Kanter M, Inan M, Aydogdu N, Basaran UN, Yalcin O, Turan FN (2007) Protective effects of ibuprofen on testicular torsion/detorsion-induced ischemia/reperfusion injury in rats. Arch Toxicol 81:655–663CrossRefPubMed

39.

Konukoglu D, Tasci I, Cetinkale O (1998) Effects of cyclosporine A and ibuprofen on liver ischemia-reperfusion injury in the rat. Clin Chim Acta 275:1–8CrossRefPubMed

40.

Rieger JM, Shah AR, Gidday JM (2002) Ischemia-reperfusion injury of retinal endothelium by cyclooxygenase- and xanthine oxidase- derved superoxide. Exp Eye Res 74:493–501CrossRefPubMed

41.

Vandivier RW, Eidsath A, Banks SM, Preas HL II, Leighton SB, Godin PJ, Suffredini AF, Danner RL (1999) Down-regulation of nitric oxide production by ibuprofen in human volunteers. J Pharmacol Exp Ther 289:1398–1403PubMed

42.

Gallego-Sandín S, Novalbos J, Rosado A, Gisbert JP, Gálvez-Múgica MA, García AG, Pajares JM, Abad-Santos F (2004) Effect of ibuprofen on cyclooxygenase and nitric oxide synthase of gastric mucosa: correlation with endoscopic lesions and adverse reactions. Dig Dis Sci 49:1538–1544CrossRefPubMed

43.

Asanuma M, Nishibayashi-Asanuma S, Miyazaki I, Kohno M, Ogawa N (2001) Neuroprotective effects of non-steroidal anti-inflammatory drugs by direct scavenging of nitric oxide radicals. J Neurochem 76:1895–1904CrossRefPubMed

44.

Akbulut H, Celik I, Ayar A, Vural P, Canbaz M (2005) Ibuprofen reduces plasma nitrite/nitrate levels in a rabbit model of endotoxin-induced shock. Neuro Endocrinol Lett 26:407–412PubMed

Title: Protective effects of ibuprofen against caustic esophageal burn injury in rats
Authors: Özkan Herek
Murat Karabul
Çiğdem Yenisey
Muhan Erkuş
Publication date: 01-07-2010
Publisher: Springer-Verlag
Published in: Pediatric Surgery International / Issue 7/2010
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-010-2618-1

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Protective effects of ibuprofen against caustic esophageal burn injury in rats

Abstract

Purpose

Materials and methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Purpose

Materials and methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 7/2010

Contralateral inguinal exploration in premature neonates: is it necessary?

Comparison of ureteroscopic procedures with rigid and semirigid ureteroscopes in pediatric population: does the caliber of instrument matter?

Laparoscopic vascular relocation: alternative treatment for renovascular hydronephrosis in children

Urological problems or fecal continence during long-term follow-up of patients with anorectal malformation

Laparoscopic cholecystocholangiography for diagnosis of prolonged jaundice in infants, experience of 144 cases

Clinical experience with a new modified transanal endorectal pull-through for Hirschsprung’s disease