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Pediatric Surgery International
Published in: Pediatric Surgery International 1/2010

01-01-2010 | Original Article

Amniotic fluid stem cell migration after intraperitoneal injection in pup rats: implication for therapy

Authors: Marco Ghionzoli, Mara Cananzi, Augusto Zani, Carlo Alberto Rossi, Francesco Fascetti Leon, Agostino Pierro, Simon Eaton, Paolo De Coppi

Published in: Pediatric Surgery International | Issue 1/2010

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Abstract

Purpose

Despite being commonly used in clinical practice, the intraperitoneal (i.p.) route has been rarely used for cell delivery. We evaluated the capacity of amniotic fluid stem (AFS) cells, administered i.p., to diffuse systemically and to integrate into tissues of healthy newborn rats.

Methods

AFS cells were obtained from pregnant GFP + Sprague–Dawley rats by c-kit selection. Wild-type Sprague–Dawley newborn rats were divided into two groups receiving i.p.: (1) 2 × 106 AFS cells (n = 12); (2) of phosphate buffer saline (PBS) (n = 2) at 24 and 48 h after birth. Animals were either killed at 96 h of life, and organs collected for gfp amplification, or at 3 weeks of life and tissues isolated for green fluorescence protein (GFP) immunofluorescence.

Results

No adverse effects were observed after i.p. injection of PBS or AFS cells. Gfp was amplified in at least one organ in all rats injected with AFS cells except one (11/12). The intestine was the organ found most frequently positive (67%) followed by liver (25%), spleen (16%), heart (16%), lungs (16%), femur (8%) and brain (0%). Immunohistochemistry confirmed PCR results.

Conclusion

In the short term, the i.p. administration of AFS cells, is a safe procedure and allows their migration, homing and integration into various organs of healthy newborn rats.
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Metadata
Title
Amniotic fluid stem cell migration after intraperitoneal injection in pup rats: implication for therapy
Authors
Marco Ghionzoli
Mara Cananzi
Augusto Zani
Carlo Alberto Rossi
Francesco Fascetti Leon
Agostino Pierro
Simon Eaton
Paolo De Coppi
Publication date
01-01-2010
Publisher
Springer-Verlag
Published in
Pediatric Surgery International / Issue 1/2010
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI
https://doi.org/10.1007/s00383-009-2504-x

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