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Pediatric Surgery International

Published in:

01-01-2010 | Original Article

Carbon dioxide modifies the morphology and function of mesothelial cells and facilitates transepithelial neuroblastoma cell migration

Authors: Yi Yu, Joachim Kuebler, Stephanie Groos, Martin Metzelder, Silvia Kurpanik, Benno Manfred Ure, Gertrud Vieten

Published in: Pediatric Surgery International | Issue 1/2010

Abstract

Background

The response of mesothelial cells to surgical trauma and bacterial contamination is poorly defined. We have recently shown that CO₂ pneumoperitoneum increases systemic metastasis of neuroblastoma cells in a murine model. Thus, we hypothesized that CO₂ alters the morphology and function of mesothelial cells and facilitates transmesothelial tumor cell migration.

Materials and methods

Murine mesothelial cells were exposed to 100% CO₂ and 5% CO₂ as control. Scanning electron microscopy (SEM) investigations, as well as LPS-induced granulocyte-colony stimulating factor (G-CSF) production and mitochondrial activity (MTT assay) were measured. Transmesothelial migration of neuroblastoma cells (Neuro2a) was determined using a transwell chamber system.

Results

CO₂ incubation was associated with a significant destruction of the microvillar formation in SEM. Migration studies showed that the barrier function of the mesothelial monolayer decreased. A significantly increased migration of neuroblastoma cells was identified after 100% CO₂ exposure (P < 0.05). Although the conversion of MTT as an indicator of mitochondrial activity was only slightly and not significantly reduced after CO₂ incubation, the release of G-CSF induced by LPS was completely blocked during the incubation with 100% CO₂ (P < 0.05). The capacity of G-CSF release recovered after the incubation.

Conclusion

We observed that peritoneal mesothelial cells lose their typical cell morphology by CO₂ incubation, which is accompanied by facilitated migration of neuroblastoma cells. Moreover, the synthesis of immunological factors is blocked, but this effect is not long lasting. These mechanisms may explain an increased metastasis rate of neuroblastoma cells after CO₂ pneumoperitoneum, which was recently observed in a murine model.

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Title: Carbon dioxide modifies the morphology and function of mesothelial cells and facilitates transepithelial neuroblastoma cell migration
Authors: Yi Yu
Joachim Kuebler
Stephanie Groos
Martin Metzelder
Silvia Kurpanik
Benno Manfred Ure
Gertrud Vieten
Publication date: 01-01-2010
Publisher: Springer-Verlag
Published in: Pediatric Surgery International / Issue 1/2010
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-009-2503-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Carbon dioxide modifies the morphology and function of mesothelial cells and facilitates transepithelial neuroblastoma cell migration

Abstract

Background

Materials and methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Materials and methods

Results

Conclusion

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