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Published in:

01-09-2018 | Original Article

Acute promyelocytic leukemia cell adhesion to vascular endothelium is reduced by heparins

Authors: Alfonso Vignoli, Marina Marchetti, Anna Falanga

Published in: Annals of Hematology | Issue 9/2018

Abstract

Adhesion of acute promyelocytic leukemia (APL) cells to endothelial cells (EC) is among the mechanisms of the APL-associated coagulopathy, responsible for early hemorrhagic deaths in affected patients. We compared the effects of dalteparin and enoxaparin, two low-molecular-weight heparins (LMWH), and unfractionated heparin (UFH), on APL NB4 adhesion to micro- (HMEC-1) and macro-vascular EC (HUVEC), in resting and interleukin-1β (IL-1β)-stimulated conditions. The heparin effect on EC adhesion molecule (ICAM-1, VCAM-1, E-selectin) expression was also assessed. In HMEC-1, dalteparin inhibited IL1β-induced NB4 adhesion by 80%, enoxaparin by 52%, and UFH by 44%. Similar results were obtained in HUVEC. This was associated with a significant decrease of VCAM-1 and ICAM-1 expression. In conclusion, we show that LMWH significantly counteract APL cell adhesion to the vessel wall, by modulating EC adhesion molecule expression. This property of heparins may represent one approach for hampering excess clotting activation and microthrombi deposition in APL.

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Title: Acute promyelocytic leukemia cell adhesion to vascular endothelium is reduced by heparins
Authors: Alfonso Vignoli
Marina Marchetti
Anna Falanga
Publication date: 01-09-2018
Publisher: Springer Berlin Heidelberg
Published in: Annals of Hematology / Issue 9/2018
Print ISSN: 0939-5555
Electronic ISSN: 1432-0584
DOI: https://doi.org/10.1007/s00277-018-3343-4

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