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01-07-2021 | Sympathectomy | Original Research

Vasoactive Intestinal Peptide Promotes Fracture Healing in Sympathectomized Mice

Authors: Liu Shi, Yang Liu, Zhengmeng Yang, Tianyi Wu, Hiu Tung Lo, Jia Xu, Jiajun Zhang, Weiping Lin, Jinfang Zhang, Lu Feng, Gang Li

Published in: Calcified Tissue International | Issue 1/2021

Abstract

Vasoactive intestinal peptide (VIP) as a neuromodulator and neurotransmitter played a significant role in modulating bone homeostasis. Our previous study reported an essential role of VIP in in vitro BMSCs osteogenesis and in vivo bone defect repair. VIP was also revealed to have a promoting effect on embryonic skeletal element development. However, the role of VIP in fracture healing is not known yet. We hypothesized that the disorder of sympathetic nervous system impairs bone structure and fracture healing, whereas VIP may rescue the sympathetic inhibition effects and promote fracture healing. We employed a 6-hydroxydopamine (6-OHDA) induced sympathectomy mice model (sympathectomized mice), in which successful sympathetic inhibition was confirmed by a decreased level of norephedrine (NE) in the spleen. In the sympathectomized mice, the femoral micro-architecture, bone density and mechanical properties were all impaired compared to the vehicle control mice. The femoral fracture was created in the vehicle or sympathectomized mice. Vehicle mice were locally injected with PBS as a negative control, and the sympathectomized mice were treated with injection of PBS or VIP. VIP expression at the fracture site was significantly decreased in sympathectomized mice. The fracture healing was repressed upon 6-OHDA treatment and rescued by VIP treatment. Micro-CT examination showed that the femoral bone micro-architecture at the fracture sites and mechanical properties were all impaired. Simultaneously, the expression level of osteogenic markers OCN and OPN were reduced in sympathectomized mice compared with vehicle group. While the VIP treatment rescued the repression effects of 6-OHDA on bone remodeling and significantly promoted bone quality and mechanical properties as well as increased osteogenesis marker expression in the sympathectomized mice. VIP administration promoted bone fracture healing by inhibiting bone resorption, making it a putative new alternative treatment strategy for fracture healing.

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Title: Vasoactive Intestinal Peptide Promotes Fracture Healing in Sympathectomized Mice
Authors: Liu Shi
Yang Liu
Zhengmeng Yang
Tianyi Wu
Hiu Tung Lo
Jia Xu
Jiajun Zhang
Weiping Lin
Jinfang Zhang
Lu Feng
Gang Li
Publication date: 01-07-2021
Publisher: Springer US
Keywords: Sympathectomy
Fracture Healing
Published in: Calcified Tissue International / Issue 1/2021
Print ISSN: 0171-967X
Electronic ISSN: 1432-0827
DOI: https://doi.org/10.1007/s00223-021-00820-9

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