Top

Published in:

01-06-2019 | Original Research

Effects of High-Intensity Interval Running Versus Cycling on Sclerostin, and Markers of Bone Turnover and Oxidative Stress in Young Men

Authors: R. Kouvelioti, P. LeBlanc, B. Falk, W. E. Ward, A. R. Josse, P. Klentrou

Published in: Calcified Tissue International | Issue 6/2019

Abstract

This study compared sclerostin’s response to impact versus no-impact high-intensity interval exercise in young men and examined the association between exercise-induced changes in sclerostin and markers of bone turnover and oxidative stress. Twenty healthy men (22.3 ± 2.3 years) performed two high-intensity interval exercise trials (crossover design); running on treadmill and cycling on cycle ergometer. Trials consisted of eight 1 min running or cycling intervals at ≥ 90% of maximal heart rate, separated by 1 min passive recovery intervals. Blood samples were collected at rest (pre-exercise), and 5 min, 1 h, 24 h, and 48 h following each trial. Serum levels of sclerostin, cross-linked telopeptide of type I collagen (CTXI), procollagen type I amino-terminal propeptide (PINP), thiobarbituric acid reactive substances (TBARS), and protein carbonyls (PC) were measured. There was no significant time or exercise mode effect for PINP and PC. A significant time effect was found for sclerostin, CTXI, and TBARS with no significant exercise mode effect and no significant time-by-mode interaction. Sclerostin increased from pre- to 5 min post-exercise (47%, p < 0.05) and returned to baseline within 1 h following the exercise. CTXI increased from pre- to 5 min post-exercise (28%, p < 0.05), then gradually returned to baseline by 48 h. TBARS did not increase significantly from pre- to 5 min post-exercise but significantly decreased from 5 min to 48 h post-exercise. There were no significant correlations between exercise-induced changes in sclerostin and any other marker. In young men, sclerostin’s response to high-intensity interval exercise is independent of impact and is not related to changes in bone turnover and oxidative stress markers.

Kohrt WM, Barry DW, Schwartz RS (2009) Muscle forces or gravity: what predominates mechanical loading on bone? Med Sci Sports Exerc 41:2050–2055. https://doi.org/10.1249/MSS.0b013e3181a8c717 CrossRefPubMedPubMedCentral

Banfi G, Lombardi G, Colombini A, Lippi G (2010) Bone metabolism markers in sports medicine. Sport Med 40:697–714. https://doi.org/10.2165/11533090-000000000-00000 CrossRef

Mezil YA, Allison D, Kish K, Ditor D, Ward WE, Tsiani E, Klentrou P (2015) Response of bone turnover markers and cytokines to high-intensity low-impact exercise. Med Sci Sports Exerc 47:1495–1502. https://doi.org/10.1249/MSS.0000000000000555 CrossRefPubMed

Kouvelioti R, Kurgan N, Falk B, Ward WE, Josse AR, Klentrou P (2018) Response of sclerostin and bone turnover markers to high-intensity interval exercise in young women: does impact matter? BioMed Res Int 2018:8CrossRef

Sapir-Koren R, Livshits G (2014) Osteocyte control of bone remodeling: is sclerostin a key molecular coordinator of the balanced bone resorption–formation cycles? Osteoporos Int 25:2685–2700. https://doi.org/10.1007/s00198-014-2808-0 CrossRefPubMed

Moester MJC, Papapoulos SE, Löwik CWGM, van Bezooijen RL (2010) Sclerostin: current knowledge and future perspectives. Calcif Tissue Int 87:99–107. https://doi.org/10.1007/s00223-010-9372-1 CrossRefPubMedPubMedCentral

Spatz J, Wein M, Gooi J, Qu Y, Garr J, Liu S, Barry K, Uda Y, Lai F, Dedic C (2015) The Wnt inhibitor sclerostin is up-regulated by mechanical unloading in osteocytes in vitro. J Biol Chem 290:16744–16758CrossRefPubMedPubMedCentral

Robling AG, Niziolek PJ, Baldridge LA, Condon KW, Allen MR, Alam I, Mantila SM, Gluhak heinrich J, Bellido TM, Harris SE, Turner CH (2008) Mechanical stimulation of bone in vivo reduces osteocyte expression of sost/sclerostin. J Biol Chem 283:5866–5875. https://doi.org/10.1074/jbc.M705092200 CrossRefPubMed

Falk B, Haddad F, Klentrou P, Ward W, Kish K, Mezil Y, Radom-Aizik S (2016) Differential sclerostin and parathyroid hormone response to exercise in boys and men. Osteoporos Int 27:1245–1249. https://doi.org/10.1007/s00198-015-3310-z CrossRefPubMed

10.

Pickering M-E, Simon M, Sornay-Rendu E, Chikh K, Carlier M-C, Raby A-L, Szulc P, Confavreux CB (2017) Serum sclerostin increases after acute physical activity. Calcif Tissue Int 101:170–173. https://doi.org/10.1007/s00223-017-0272-5 CrossRefPubMed

11.

Gombos GC, Bajsz V, Pék E, Schmidt B, Sió E, Molics B, Betlehem J (2016) Direct effects of physical training on markers of bone metabolism and serum sclerostin concentrations in older adults with low bone mass. BMC Musculoskelet Disord 17:254. https://doi.org/10.1186/s12891-016-1109-5 CrossRefPubMedPubMedCentral

12.

Kerschan-Schindl K, Thalmann MM, Weiss E, Tsironi M, Föger-Samwald U, Meinhart J, Skenderi K, Pietschmann P (2015) Changes in serum levels of myokines and Wnt-antagonists after an ultramarathon race. PLoS ONE 10:e0132478. https://doi.org/10.1371/journal.pone.0132478 CrossRefPubMedPubMedCentral

13.

Costa AG, Walker MD, Zhang CA, Cremers S, Dworakowski E, McMahon DJ, Liu G, Bilezikian JP (2013) Circulating sclerostin levels and markers of bone turnover in chinese-american and white women. J Clin Endocrinol Metab 98:4736–4743. https://doi.org/10.1210/jc.2013-2106 CrossRefPubMedPubMedCentral

14.

Durosier C, van Lierop A, Ferrari S, Chevalley T, Papapoulos S, Rizzoli R (2013) Association of circulating sclerostin with bone mineral mass, microstructure, and turnover biochemical markers in healthy elderly men and women. J Clin Endocrinol Metab 98:3873–3883. https://doi.org/10.1210/jc.2013-2113 CrossRefPubMed

15.

Gaudio A, Pennisi P, Bratengeier C, Torrisi V, Lindner B, Mangiafico RA, Pulvirenti I, Hawa G, Tringali G, Fiore CE (2010) Increased sclerostin serum levels associated with bone formation and resorption markers in patients with immobilization-induced bone loss. J Clin Endocrinol Metab 95:2248–2253. https://doi.org/10.1210/jc.2010-0067 CrossRefPubMed

16.

Kawamura T, Muraoka I (2018) Exercise-induced oxidative stress and the effects of antioxidant intake from a physiological viewpoint. Antioxidants 7:119. https://doi.org/10.3390/antiox7090119 CrossRefPubMedCentral

17.

Wauquier F, Leotoing L, Coxam V, Guicheux J, Wittrant Y (2009) Oxidative stress in bone remodelling and disease. Trends Mol Med 15:468–477. https://doi.org/10.1016/j.molmed.2009.08.004 CrossRefPubMed

18.

Manolagas SC, Almeida M (2007) Gone with the Wnts: beta-catenin, T-cell factor, forkhead box O, and oxidative stress in age-dependent diseases of bone, lipid, and glucose metabolism. Mol Endocrinol 21:2605–2614. https://doi.org/10.1210/me.2007-0259 CrossRefPubMed

19.

Kang J, Boonanantanasarn K, Baek K, Woo KM, Ryoo H-M, Baek J h, Kim G-S (2015) Hyperglycemia increases the expression levels of sclerostin in a reactive oxygen species- and tumor necrosis factor-alpha-dependent manner. J Periodontal Implant Sci 45:101. https://doi.org/10.5051/jpis.2015.45.3.101 CrossRefPubMedPubMedCentral

20.

Fisher-Wellman K, Bloomer RJ (2009) Acute exercise and oxidative stress: a 30 year history. Dyn Med 8:1. https://doi.org/10.1186/1476-5918-8-1 CrossRefPubMedPubMedCentral

21.

Bloomer RJ, Goldfarb AH (2004) Anaerobic exercise and oxidative stress: a review. Can J Appl Physiol 29:245–263. https://doi.org/10.1139/h04-017 CrossRefPubMed

22.

Steinberg JG, Ba A, Brégeon F, Delliaux S, Jammes Y (2007) Cytokine and oxidative responses to maximal cycling exercise in sedentary subjects. Med Sci Sports Exerc 39:964–968. https://doi.org/10.1097/mss.0b013e3180398f4b CrossRefPubMed

23.

Bloomer R, Goldfarb A, Wideman L, McKenzie M, Consitt L (2005) Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress. J Strength Cond Res 19:276. https://doi.org/10.1519/14823.1 CrossRefPubMed

24.

Borg GA (1973) Perceived exertion: a note on “history” and methods. Med Sci Sports 5:90–93PubMed

25.

Levine RL, Williams JA, Stadtman EP, Shacter E (1994) [37] Carbonyl assays for determination of oxidatively modified proteins. Methods Enzymol 233:346–357. https://doi.org/10.1016/S0076-6879(94)33040-9 CrossRef

26.

Reznick AZ, Packer L (1994) Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol 233:357–363CrossRefPubMed

27.

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. https://doi.org/10.1016/0003-2697(76)90527-3 CrossRef

28.

Kei S (1978) Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin Chim Acta 90:37–43. https://doi.org/10.1016/0009-8981(78)90081-5 CrossRef

29.

Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358. https://doi.org/10.1016/0003-2697(79)90738-3 CrossRef

30.

Kargotich S, Goodman C, Keast D, Morton AR (1998) The influence of exercise-induced plasma volume changes on the interpretation of biochemical parameters used for monitoring exercise, training and sport. Sport Med 26:101–117. https://doi.org/10.2165/00007256-199826020-00004 CrossRef

31.

Weinstein Y, Bediz C, Dotan R, Falk B (1998) Reliability of peak-lactate, heart rate, and plasma volume following the Wingate test. Med Sci Sports Exerc 30:1456–1460PubMed

32.

Van Beaumont W (1972) Evaluation of hemoconcentration from hematocrit measurements. J Appl Physiol 32:712–713. https://doi.org/10.1152/jappl.1972.32.5.712 CrossRefPubMed

33.

Rogers RS, Dawson AW, Wang Z, Thyfault JP, Hinton PS (2011) Acute response of plasma markers of bone turnover to a single bout of resistance training or plyometrics. J Appl Physiol 111:1353–1360. https://doi.org/10.1152/japplphysiol.00333.2011 CrossRefPubMed

34.

van Beaumont W, Strand JC, Petrofsky JS, Hipskind SG, Greenleaf JE (1973) Changes in total plasma content of electrolytes and proteins with maximal exercise. J Appl Physiol 34:102–106. https://doi.org/10.1152/jappl.1973.34.1.102 CrossRefPubMed

35.

de Oliveira Teixeira A, Franco OS, Borges MM, Martins CN, Guerreiro LF, da Rosa CE, da Silva Paulitsch F, Perez W, da Silva AM, Signori LU (2014) The importance of adjustments for changes in plasma volume in the interpretation of hematological and inflammatory responses after resistance exercise. J Exerc Physiol 17:72–83

36.

Kirmani S, Amin S, McCready LK, Atkinson EJ, Melton LJ, Müller R, Khosla S (2012) Sclerostin levels during growth in children. Osteoporos Int 23:1123–1130. https://doi.org/10.1007/s00198-011-1669-z CrossRefPubMed

37.

Mödder UI, Hoey KA, Amin S, McCready LK, Achenbach SJ, Riggs BL, Melton LJ, Khosla S (2011) Relation of age, gender, and bone mass to circulating sclerostin levels in women and men. J Bone Miner Res 26:373–379. https://doi.org/10.1002/jbmr.217 CrossRefPubMed

38.

Dekker J, Nelson K, Kurgan N, Falk B, Josse A, Klentrou P (2017) Wnt signaling–related osteokines and transforming growth factors before and after a single bout of plyometric exercise in child and adolescent females. Pediatr Exerc Sci 29:504–512. https://doi.org/10.1123/pes.2017-0042 CrossRefPubMed

39.

Klentrou P, Angrish K, Awadia N, Kurgan N, Kouvelioti R, Falk B (2018) Wnt signaling–related osteokines at rest and following plyometric exercise in prepubertal and early pubertal boys and girls. Pediatr Exerc Sci. https://doi.org/10.1123/pes.2017-0259 CrossRefPubMed

40.

Heinonen I, Kemppainen J, Kaskinoro K, Langberg H, Knuuti J, Boushel R, Kjaer M, Kalliokoski KK (2013) Bone blood flow and metabolism in humans: effect of muscular exercise and other physiological perturbations. J Bone Miner Res 28:1068–1074. https://doi.org/10.1002/jbmr.1833 CrossRefPubMed

41.

Farquhar B, Kenney W (1997) Anti-inflamatory drugs, kidney function, and exercise. Sport Sci Exch 11:1–6

42.

Fairfield H, Rosen CJ, Reagan MR (2017) Connecting bone and fat: the potential role for sclerostin. Curr Mol Biol Rep 3:114–121. https://doi.org/10.1007/s40610-017-0057-7 CrossRefPubMedPubMedCentral

43.

Fulzele K, Lai F, Dedic C, Saini V, Uda Y, Shi C, Tuck P, Aronson JL, Liu X, Spatz JM, Wein MN, Divieti Pajevic P (2017) Osteocyte-secreted Wnt signaling inhibitor sclerostin contributes to beige adipogenesis in peripheral fat depots. J Bone Miner Res 32:373–384. https://doi.org/10.1002/jbmr.3001 CrossRefPubMedPubMedCentral

44.

Morgan AL, Weiss J, Kelley ET (2015) Bone turnover response to acute exercise with varying impact levels: a preliminary investigation. Int J Exerc Sci 8:154–163

45.

Ardawi M-SM, Rouzi AA, Qari MH (2012) Physical activity in relation to serum sclerostin, insulin-like growth factor-1, and bone turnover markers in healthy premenopausal women: a cross-sectional and a longitudinal study. J Clin Endocrinol Metab 97:3691–3699. https://doi.org/10.1210/jc.2011-3361 CrossRefPubMed

46.

Grasso D, Corsetti R, Lanteri P, Di Bernardo C, Colombini A, Graziani R, Banfi G, Lombardi G (2015) Bone-muscle unit activity, salivary steroid hormones profile, and physical effort over a 3-week stage race. Scand J Med Sci Sports 25:70–80. https://doi.org/10.1111/sms.12147 CrossRefPubMed

47.

Gaeini AA, Rahnama N, Hamedinia MR (2006) Effects of vitamin E supplementation on oxidative stress at rest and after exercise to exhaustion in athletic students. J Sports Med Phys Fitness 46:458–461PubMed

48.

Bloomer R, Davis P, Consitt L, Wideman L (2007) Plasma protein carbonyl response to increasing exercise duration in aerobically trained men and women. Int J Sports Med 28:21–25. https://doi.org/10.1055/s-2006-924140 CrossRefPubMed

49.

Chevion S, Moran DS, Heled Y, Shani Y, Regev G, Abbou B, Berenshtein E, Stadtman ER, Epstein Y (2003) Plasma antioxidant status and cell injury after severe physical exercise. Proc Natl Acad Sci USA 100:5119–5123. https://doi.org/10.1073/pnas.0831097100 CrossRefPubMed

50.

Urso ML, Clarkson PM (2003) Oxidative stress, exercise, and antioxidant supplementation. Toxicology 189:41–54CrossRefPubMed

51.

Veskoukis AS, Nikolaidis MG, Kyparos A, Kouretas D (2009) Blood reflects tissue oxidative stress depending on biomarker and tissue studied. Free Radic Biol Med 47:1371–1374. https://doi.org/10.1016/J.FREERADBIOMED.2009.07.014 CrossRefPubMed

52.

Parker L, Trewin A, Levinger I, Shaw CS, Stepto NK (2018) Exercise-intensity dependent alterations in plasma redox status do not reflect skeletal muscle redox-sensitive protein signaling. J Sci Med Sport 21:416–421. https://doi.org/10.1016/J.JSAMS.2017.06.017 CrossRefPubMed

53.

Ashton T, Rowlands CC, Jones E, Young IS, Jackson SK, Davies B, Peters JR (1998) Electron spin resonance spectroscopic detection of oxygen-centred radicals in human serum following exhaustive exercise. Eur J Appl Physiol 77:498–502. https://doi.org/10.1007/s004210050366 CrossRef

Title: Effects of High-Intensity Interval Running Versus Cycling on Sclerostin, and Markers of Bone Turnover and Oxidative Stress in Young Men
Authors: R. Kouvelioti
P. LeBlanc
B. Falk
W. E. Ward
A. R. Josse
P. Klentrou
Publication date: 01-06-2019
Publisher: Springer US
Published in: Calcified Tissue International / Issue 6/2019
Print ISSN: 0171-967X
Electronic ISSN: 1432-0827
DOI: https://doi.org/10.1007/s00223-019-00524-1

ACC 2024 Congress Coverage

Heart Failure Video

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Effects of High-Intensity Interval Running Versus Cycling on Sclerostin, and Markers of Bone Turnover and Oxidative Stress in Young Men

Abstract

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 6/2019

Bone Turnover Markers in Men and Women with Impaired Fasting Glucose and Diabetes

Prospective Follow-Up of Cortical Interruptions, Bone Density, and Micro-structure Detected on HR-pQCT: A Study in Patients with Rheumatoid Arthritis and Healthy Subjects

Elevated Bone Remodeling Markers of CTX and P1NP in Addition to Sclerostin in Patients with X-linked Hypophosphatemia: A Cross-Sectional Controlled Study

Interleukin-6 Inhibitor Suppresses Hyperalgesia Without Improvement in Osteoporosis in a Mouse Pain Model of Osteoporosis

A Comprehensive Study of Bone Manifestations in Adult Gaucher Disease Type 1 Patients in Argentina

The Association of Vitamin D in Youth and Early Adulthood with Bone Mineral Density and Microarchitecture in Early Adulthood

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page