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Intensive Care Medicine

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01-05-2007 | Experimental

Infusions of rocuronium and cisatracurium exert different effects on rat diaphragm function

Authors: Dries Testelmans, Karen Maes, Patrick Wouters, Scott K. Powers, Marc Decramer, Ghislaine Gayan-Ramirez

Published in: Intensive Care Medicine | Issue 5/2007

Abstract

Objective

Aminosteroidal and benzylisoquinoline neuromuscular blocking agents are used in the intensive care unit to facilitate mechanical ventilation. The use of these agents has been associated with development of critical illness myopathy; however, the relative frequency of myopathy development among agents is not known. The aim of our study was to compare the effects of 24 h infusion of rocuronium or cisatracurium on the diaphragm in mechanically ventilated rats.

Design

Randomized, controlled experiment.

Setting

Basic animal science laboratory.

Subjects

Male Wistar rats, 14 weeks old.

Interventions

Rats were divided into four groups to receive either saline, rocuronium (low dose) or cisatracurium (low or high dose).

Measurements and results

After 24 h, in vitro diaphragm tetanic force was decreased after rocuronium (–33% vs. saline), while the force was more preserved after cisatracurium, even in the high-dose group. Cross-sectional areas of the different diaphragm and gastrocnemius fibers were unaltered. Diaphragmatic MURF-1 mRNA was increased after rocuronium (+44% vs. saline), while unchanged in both cisatracurium groups. Calpain activity was increased after rocuronium (+75% vs. saline) and unchanged in the cisatracurium groups. MURF-1 mRNA expression and calpain activity were negatively correlated with diaphragm force.

Conclusions

Cisatracurium infusion during controlled mechanical ventilation exerted less detrimental effects on diaphragm function and proteolytic activity than infusion of rocuronium, even with the higher effective dose. These data suggest that increased calpain activity and increased activation of the ubiquitin proteasome system play a role in the different effects of these agents.

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Title: Infusions of rocuronium and cisatracurium exert different effects on rat diaphragm function
Authors: Dries Testelmans
Karen Maes
Patrick Wouters
Scott K. Powers
Marc Decramer
Ghislaine Gayan-Ramirez
Publication date: 01-05-2007
Publisher: Springer Berlin Heidelberg
Published in: Intensive Care Medicine / Issue 5/2007
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-007-0584-4

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Infusions of rocuronium and cisatracurium exert different effects on rat diaphragm function

Abstract

Objective

Design

Setting

Subjects

Interventions

Measurements and results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Objective

Design

Setting

Subjects

Interventions

Measurements and results

Conclusions

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