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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

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01-09-2018 | Original Article

Expression kinetics of human periodontal ligament fibroblasts in the early phases of orthodontic tooth movement

Authors: Dr. rer. nat. Agnes Schröder, Dipl.-Biol. Kathrin Bauer, Dr. Gerrit Spanier, MD, DDS, Professor Dr. Dr. Peter Proff, MD, DDS, (PhD), Professor Dr. Michael Wolf, DDS, (PhD), PD Dr. Christian Kirschneck, DDS, (PhD)

Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie | Issue 5/2018

Abstract

Purpose

Human periodontal ligament (hPDL) fibroblasts play a crucial mediating role in orthodontic tooth movement (OTM). In this study, we investigated the expression kinetics of genes associated with OTM in its early phase to obtain better insight into the timing and regulation of molecular and cellular signalling and transformation processes occurring in compressive areas of the periodontal ligament during OTM.

Methods

Adherent hPDL fibroblasts were stimulated with physiological orthodontic compressive forces of 2 g/cm² for 24, 48, 72, and 96 h under cell culture conditions. At each time point, we quantified relative gene expression of genes involved in bone remodelling (ALPL), inflammation (COX2, IL-6), extracellular matrix reorganization (COL1A2, P4HA1, FN1, MMP8) and angiogenesis (VEGF-A) by means of RT-qPCR as well as protein expression of osteoclastogenesis-regulating RANK-L and OPG relative to pressure-untreated controls incubated for corresponding time periods. In addition, coculture experiments with osteoclast precursor cells were performed to determine the extent of hPDL-fibroblast-mediated osteoclastogenesis (TRAP staining).

Results

As primary response to compressive forces within 24 h, we observed an induction of genes associated with angiogenesis, inflammation, osteoblastogenesis, and the remodelling of the extracellular matrix, with RANK-L expression at first slightly inhibited and only increased after 48 h. Major hPDL-mediated osteoclastogenesis was observed after 72 h with minor, non-RANK-L-dependent osteoclastogenesis occurring as early as 24 h after compressive force application.

Conclusions

hPDL fibroblasts seem to play a major mediating role in the early phase of OTM with a differentiated, time-dependent regulation and expression pattern of cytokines and other mediators.

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Title: Expression kinetics of human periodontal ligament fibroblasts in the early phases of orthodontic tooth movement
Authors: Dr. rer. nat. Agnes Schröder
Dipl.-Biol. Kathrin Bauer
Dr. Gerrit Spanier, MD, DDS
Professor Dr. Dr. Peter Proff, MD, DDS, (PhD)
Professor Dr. Michael Wolf, DDS, (PhD)
PD Dr. Christian Kirschneck, DDS, (PhD)
Publication date: 01-09-2018
Publisher: Springer Medizin
Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie / Issue 5/2018
Print ISSN: 1434-5293
Electronic ISSN: 1615-6714
DOI: https://doi.org/10.1007/s00056-018-0145-1

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Expression kinetics of human periodontal ligament fibroblasts in the early phases of orthodontic tooth movement

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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