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Inflammation Research

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01-07-2020 | Short Communication

CHRFAM7A reduces monocyte/macrophage migration and colony formation in vitro

Authors: Theresa W. Chan, Simone Langness, Olga Cohen, Brian P. Eliceiri, Andrew Baird, Todd W. Costantini

Published in: Inflammation Research | Issue 7/2020

Abstract

Objective and design

CHRFAM7A is a unique human gene that encodes a dominant negative inhibitor of the α7 nicotinic acetylcholine receptor. We have recently shown that CHRFAM7A is expressed in human leukocytes, increases cel–cell adhesion, and regulates the expression of genes associated with leukocyte migration.

Material

Human THP-1, RAW264.7 and HEK293 cells.

Methods

Cell migration, cell proliferation and colony formation in soft agar to compare the biological activity of vector vs. CHRFAM7A-transduced cells.

Results

We show that gene delivery of CHRFAM7A into the THP-1 human monocytic cell line reduces cell migration, reduces chemotaxis to monocyte chemoattractant protein, and reduces colony formation in soft agar.

Conclusion

Taken together, the findings demonstrate that CHRFAM7A regulates the biological activity of monocytes/macrophages to migrate and undergo anchorage-independent growth in vitro.

Available only for authorised users

Costantini TW, Dang X, Coimbra R, Eliceiri BP, Baird A. CHRFAM7A, a human-specific and partially duplicated alpha7-nicotinic acetylcholine receptor gene with the potential to specify a human-specific inflammatory response to injury. J Leukoc Biol. 2015;97(2):247–57. https://doi.org/10.1189/jlb.4RU0814-381R.CrossRefPubMed

Tracey KJ. The inflammatory reflex. Nature. 2002;420(6917):853–9.CrossRef

Costantini TW, Dang X, Yurchyshyna MV, Coimbra R, Eliceiri BP, Baird A. A human-specific alpha7-nicotinic acetylcholine receptor gene in human leukocytes: identification, regulation and the consequences of CHRFAM7A expression. Mol Med. 2015;21:323–36. https://doi.org/10.2119/molmed.2015.00018.CrossRefPubMedPubMedCentral

Costantini TW, Chan TW, Cohen O, Langness S, Treadwell S, Williams E, et al. Uniquely human CHRFAM7A gene increases the hematopoietic stem cell reservoir in mice and amplifies their inflammatory response. Proc Natl Acad Sci USA. 2019;116(16):7932–40. https://doi.org/10.1073/pnas.1821853116.CrossRefPubMed

Daly AC, Randall RA, Hill CS. Transforming growth factor beta-induced Smad1/5 phosphorylation in epithelial cells is mediated by novel receptor complexes and is essential for anchorage-independent growth. Mol Cell Biol. 2008;28(22):6889–902. https://doi.org/10.1128/MCB.01192-08.CrossRefPubMedPubMedCentral

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Yu B, Sondag GR, Malcuit C, Kim MH, Safadi FF. Macrophage-associated osteoactivin/GPNMB Mediates Mesenchymal Stem Cell Survival, Proliferation, And Migration Via a CD44-dependent mechanism. J Cell Biochem. 2016;117(7):1511–21. https://doi.org/10.1002/jcb.25394.CrossRefPubMed

Halevi S, Yassin L, Eshel M, Sala F, Sala S, Criado M, et al. Conservation within the RIC-3 gene family—effectors of mammalian nicotinic acetylcholine receptor expression. J Biol Chem. 2003;278(36):34411–7. https://doi.org/10.1074/jbc.M300170200.CrossRefPubMed

Title: CHRFAM7A reduces monocyte/macrophage migration and colony formation in vitro
Authors: Theresa W. Chan
Simone Langness
Olga Cohen
Brian P. Eliceiri
Andrew Baird
Todd W. Costantini
Publication date: 01-07-2020
Publisher: Springer International Publishing
Published in: Inflammation Research / Issue 7/2020
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-020-01349-7

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CHRFAM7A reduces monocyte/macrophage migration and colony formation in vitro

Abstract

Objective and design

Material

Methods

Results

Conclusion

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Abstract

Objective and design

Material

Methods

Results

Conclusion

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