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01-12-2017 | Original Research Paper

Ouabain attenuates ovalbumin-induced airway inflammation

Authors: José Guilherme F. M. Galvão, Luiz Henrique Agra Cavalcante-Silva, Deyse Cristina M. Carvalho, Laércia Karla D. P. Ferreira, Talissa Mozzini Monteiro, Adriano Francisco Alves, Larissa Adilis M. P. Ferreira, Francisco Allysson A. F. Gadelha, Marcia Regina Piuvezam, Sandra Rodrigues-Mascarenhas

Published in: Inflammation Research | Issue 12/2017

Abstract

Purpose

Ouabain, an Na⁺/K⁺-ATPase inhibitor hormone, presents immunomodulatory actions, including anti-inflammatory effect on acute inflammation models.

Methods

In the present study, the effect of ouabain in a model of allergic airway inflammation induced by ovalbumin (OVA) was assessed.

Results

Initially, it was observed that ouabain treatment inhibited cellular migration induced by OVA on bronchoalveolar lavage fluid (BALF), mostly granulocytes, without modulating macrophage migration. In addition, it was observed, by flow cytometry, that ouabain reduces CD3^high lymphocytes cells on BALF. Furthermore, treatment with ouabain decreased IL-4 and IL-13 levels on BALF. Ouabain also promoted pulmonary histological alterations, including decreased cell migration into peribronchiolar and perivascular areas, and reduced mucus production in bronchioles regions observed through hematoxylin–eosin (HE) and by periodic acid-Schiff stain, respectively. Allergic airway inflammation is characterized by high OVA-specific IgE serum titer. This parameter was also reduced by the treatment with ouabain.

Conclusions

Therefore, our data demonstrate that ouabain negatively modulates allergic airway inflammation induced by OVA.

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Title: Ouabain attenuates ovalbumin-induced airway inflammation
Authors: José Guilherme F. M. Galvão
Luiz Henrique Agra Cavalcante-Silva
Deyse Cristina M. Carvalho
Laércia Karla D. P. Ferreira
Talissa Mozzini Monteiro
Adriano Francisco Alves
Larissa Adilis M. P. Ferreira
Francisco Allysson A. F. Gadelha
Marcia Regina Piuvezam
Sandra Rodrigues-Mascarenhas
Publication date: 01-12-2017
Publisher: Springer International Publishing
Published in: Inflammation Research / Issue 12/2017
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-017-1092-9

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Ouabain attenuates ovalbumin-induced airway inflammation

Abstract

Purpose

Methods

Results

Conclusions

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Abstract

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Conclusions

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