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Inflammation Research
Published in: Inflammation Research 6/2014

01-06-2014 | Original Research Paper

Differential effects of low and high doses of lipoteichoic acid on lipopolysaccharide-induced interleukin-6 production

Authors: Hangeun Kim, Bong Jun Jung, Joo Yun Kim, Dae Kyun Chung

Published in: Inflammation Research | Issue 6/2014

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Abstract

Objective

Interleukin-6 (IL-6), which is increased in patients who are suffering from septic shock, is an important mediator of the inflammatory response. Here, we examined the priming effect of lipoteichoic acid (LTA) and lipopolysaccharide (LPS) on IL-6 production in a monocyte-like cell line.

Methods

THP-1 cells were primed by treatingwith a low or high dose of LTA isolated from Staphylococcus aureus (aLTA) and then re-treated with LPS. IL-6 production, receptor expression, and the variation of signaling molecules were examined by ELISA, reverse transcriptase polymerase chain reaction, and western blotting, respectively.

Results

LPS-mediated IL-6 production was dramatically increased in THP-1 cells pretreated with a low dose aLTA, while it was significantly decreased when a high dose of aLTA was given along with LPS. LPS-induced IL-6 production in low dose aLTA priming cells mediated by NF-κB and MAPKs pathways, and Akt functioned as a negative regulator of IL-6 production. Together, the results of this study suggest that different doses of bacterial cell surface components can mediate a diverse range of responses with respect to inflammatory cytokine production.
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Metadata
Title
Differential effects of low and high doses of lipoteichoic acid on lipopolysaccharide-induced interleukin-6 production
Authors
Hangeun Kim
Bong Jun Jung
Joo Yun Kim
Dae Kyun Chung
Publication date
01-06-2014
Publisher
Springer Basel
Published in
Inflammation Research / Issue 6/2014
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-014-0714-8

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