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01-03-2012 | Original Research Paper

Leishmania (Viannia) shawi purified antigens confer protection against murine cutaneous leishmaniasis

Authors: Luiz Felipe Domingues Passero, Ana Kely Carvalho, Maria Luiza A. C. Bordon, Alexis Bonfim-Melo, Marcos Hikari Toyama, Carlos Eduardo Pereira Corbett, Márcia Dalastra Laurenti

Published in: Inflammation Research | Issue 3/2012

Abstract

Objective

Leishmania (Viannia) shawi was characterized only recently, and few studies concerning the immunogenic and protective properties of its antigens have been performed. The present study aimed to evaluate the protective potential of the five antigenic fractions isolated from L. (V.) shawi promastigotes in experimental cutaneous leishmaniasis.

Materials and methods

Soluble antigen from L. (V.) shawi promastigotes was submitted to reverse phase HPLC to purify F1, F2, F3, F4 and F5 antigens. BALB/c mice were immunized once a week for two consecutive weeks by subcutaneous routes in the rump, using 25 μg protein. After 1 week, groups were challenged in the footpad with L. (V.) shawi promastigotes. After 8 weeks, those same mice were sacrificed and parasite burden as well as the cellular and humoral immune responses were evaluated.

Results

F1 and F5-immunized mice restrained lesion progression and parasite load in the skin. However, only the F1 group was able to control the parasitism in lymph nodes, which was associated with low IL-4 and high IFN-γ production; IgG2a isotype was increased in this group. Immunizations with F2, F3 and F4 antigens did not protect mice.

Conclusion

The capability of antigens to restrain IL-4 levels and increase IFN-γ was associated with protection, such as in immunization using F1 antigen.

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Title: Leishmania (Viannia) shawi purified antigens confer protection against murine cutaneous leishmaniasis
Authors: Luiz Felipe Domingues Passero
Ana Kely Carvalho
Maria Luiza A. C. Bordon
Alexis Bonfim-Melo
Marcos Hikari Toyama
Carlos Eduardo Pereira Corbett
Márcia Dalastra Laurenti
Publication date: 01-03-2012
Publisher: SP Birkhäuser Verlag Basel
Published in: Inflammation Research / Issue 3/2012
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-011-0407-5

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Leishmania (Viannia) shawi purified antigens confer protection against murine cutaneous leishmaniasis

Abstract

Objective

Materials and methods

Results

Conclusion

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Conclusion

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