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Inflammation Research
Published in: Inflammation Research 3/2012

01-03-2012 | Original Research Paper

Severe neutrophil-mediated lung inflammation in myeloperoxidase-deficient mice exposed to zymosan

Authors: Kazuhiro Takeuchi, Yu Umeki, Noriko Matsumoto, Kei Yamamoto, Mina Yoshida, Kazuo Suzuki, Yasuaki Aratani

Published in: Inflammation Research | Issue 3/2012

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Abstract

Objective and design

This study examines the role of myeloperoxidase (MPO), a major constituent of neutrophils that generates hypochlorous acid, in neutrophil recruitment into the zymosan-exposed lung of mice.

Methods

Mice were inoculated intranasally with zymosan. The accumulation of neutrophils and other inflammatory cells within the lung was analyzed by flow cytometry. Macrophage inflammatory protein 2 (MIP-2) expression in the lung was quantified, and the contribution of this chemokine to neutrophil accumulation was examined by intranasal administration of MIP-2 antibody. The cellular sources of MIP-2 were identified, and the production of this chemokine from macrophages and neutrophils was quantified in vitro.

Results

Zymosan exposure led to greater neutrophil infiltration into the lungs of MPO−/− mice relative to wild-type mice. This was associated with higher MIP-2 levels in the mutant mice. Neutralization of MIP-2 in vivo significantly reduced neutrophil infiltration. Neutrophils from MPO−/− mice produced more MIP-2, and the production was reduced when MPO was added exogenously.

Conclusions

MPO deficiency results in severe lung inflammation in mice exposed to zymosan. Relatively high MIP-2 levels likely contribute to the strong inflammatory response in these animals.
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Metadata
Title
Severe neutrophil-mediated lung inflammation in myeloperoxidase-deficient mice exposed to zymosan
Authors
Kazuhiro Takeuchi
Yu Umeki
Noriko Matsumoto
Kei Yamamoto
Mina Yoshida
Kazuo Suzuki
Yasuaki Aratani
Publication date
01-03-2012
Publisher
SP Birkhäuser Verlag Basel
Published in
Inflammation Research / Issue 3/2012
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-011-0401-y

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