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Open Access 13-03-2024 | Herpes Simplex Type 1

RNA-Seq time-course analysis of neural precursor cell transcriptome in response to herpes simplex Virus-1 infection

Authors: Joel A. Wood, Srilakshmi Chaparala, Cecilia Bantang, Ansuman Chattopadhyay, Maribeth A. Wesesky, Paul R. Kinchington, Vishwajit L. Nimgaonkar, David C. Bloom, Leonardo D’Aiuto

Published in: Journal of NeuroVirology

Abstract

The neurogenic niches within the central nervous system serve as essential reservoirs for neural precursor cells (NPCs), playing a crucial role in neurogenesis. However, these NPCs are particularly vulnerable to infection by the herpes simplex virus 1 (HSV-1). In the present study, we investigated the changes in the transcriptome of NPCs in response to HSV-1 infection using bulk RNA-Seq, compared to those of uninfected samples, at different time points post infection and in the presence or absence of antivirals. The results showed that NPCs upon HSV-1 infection undergo a significant dysregulation of genes playing a crucial role in aspects of neurogenesis, including genes affecting NPC proliferation, migration, and differentiation. Our analysis revealed that the CREB signaling, which plays a crucial role in the regulation of neurogenesis and memory consolidation, was the most consistantly downregulated pathway, even in the presence of antivirals. Additionally, cholesterol biosynthesis was significantly downregulated in HSV-1-infected NPCs. The findings from this study, for the first time, offer insights into the intricate molecular mechanisms that underlie the neurogenesis impairment associated with HSV-1 infection.

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Title: RNA-Seq time-course analysis of neural precursor cell transcriptome in response to herpes simplex Virus-1 infection
Authors: Joel A. Wood
Srilakshmi Chaparala
Cecilia Bantang
Ansuman Chattopadhyay
Maribeth A. Wesesky
Paul R. Kinchington
Vishwajit L. Nimgaonkar
David C. Bloom
Leonardo D’Aiuto
Publication date: 13-03-2024
Publisher: Springer International Publishing
Keywords: Herpes Simplex Type 1
Herpes Virus
Published in: Journal of NeuroVirology
Print ISSN: 1355-0284
Electronic ISSN: 1538-2443
DOI: https://doi.org/10.1007/s13365-024-01198-8

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RNA-Seq time-course analysis of neural precursor cell transcriptome in response to herpes simplex Virus-1 infection

Abstract

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Springer Medicine

Abstract

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