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Open Access 01-12-2023 | Laser | Original Article

Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress

Authors: Jiaxi Zhang, Xizhong Zhang, Kaifang Han, Xuan Wang, Ziyuan Guo, Qi Deng, Jiahui Li, Shuxin Lv, Wenwen Yu

Published in: Lasers in Medical Science | Issue 1/2023

Abstract

This study aimed to investigate the effect of Low-Level Laser Therapy (LLLT) on human Periodontal Ligament Cells (hPDLCs) under tension stress. Primary hPDLCs were obtained using the tissue culture method, and P3 cells were utilized for the subsequent experiments. The study comprised four groups: a blank control group (Group B), a laser irradiation group (Group L), a tension stress group (Group T), and a laser + tension stress group (Group LT). Mechanical loading was applied using an in-vitro cell stress loading device at a frequency of 0.5 Hz and deformation of 2% for two hours per day for two days. Laser irradiation at 808 nm GaAlAs laser was administered 1 h after force loading. Cell samples were collected after the experiment. Bone and fiber remodeling factors were analyzed using PCR and Western blot. Flow cytometry was employed to assess the cell cycle, while ROS and Ca²⁺ levels were measured using a multifunctional enzyme labeling instrument. The results revealed that laser intervention under tension stress inhibited the expression of osteogenic differentiation factors, promoted the expression of osteoclast differentiation factors, and significantly increased the production of collagen factors, MMPs, and TIMPs. The LT group exhibited the most active cell cycle (P < 0.05). LLLT not only enhanced Ca²⁺ expression in hPDLCs under tension stress, but also stimulated the production of ROS. Overall, our findings demonstrate that LLLT effectively accelerated the proliferation of hPDLCs and the remodeling of periodontal tissue, possibly through the regulation of ROS and Ca²⁺ levels in hPDLCs.

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Title: Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress
Authors: Jiaxi Zhang
Xizhong Zhang
Kaifang Han
Xuan Wang
Ziyuan Guo
Qi Deng
Jiahui Li
Shuxin Lv
Wenwen Yu
Publication date: 01-12-2023
Publisher: Springer London
Keyword: Laser
Published in: Lasers in Medical Science / Issue 1/2023
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-023-03885-0

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Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress

Abstract

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Abstract

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