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Lasers in Medical Science
Published in: Lasers in Medical Science 1/2012

01-01-2012 | Original Article

Metrical and histological investigation of the effects of low-level laser therapy on orthodontic tooth movement

Authors: Burcu Ayse Altan, Oral Sokucu, Mahmud M. Ozkut, Sevinc Inan

Published in: Lasers in Medical Science | Issue 1/2012

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Abstract

The aim of this study was to evaluate the effects of 820-nm diode laser on osteoclastic and osteoblastic cell proliferation-activity and RANKL/OPG release during orthodontic tooth movement. Thirty-eight albino Wistar rats were used for this experiment. Maxillary incisors of the subjects were moved orthodontically by a helical spring with force of 20 g. An 820-nm Ga-Al-As diode laser with an output power of 100 mW and a fiber probe with spot size of 2 mm in diameter were used for laser treatment and irradiations were performed on 5 points at the distal side of the tooth root on the first, second, and 3rd days of the experiment. Total laser energy of 54 J (100 mW, 3.18 W/cm2, 1717.2 J/cm2) was applied to group II and a total of 15 J (100 mW, 3.18 W/cm2, 477 J/cm2) to group III. The experiment lasted for 8 days. The number of osteoclasts, osteoblasts, inflammatory cells and capillaries, and new bone formation were evaluated histologically. Besides immunohistochemical staining of PCNA, RANKL and OPG were also performed. No statistical difference was found for the amount of tooth movement in between the control and study groups (p > 0.05). The number of osteoclasts, osteoblasts, inflammatory cells, capillary vascularization, and new bone formation were found to be increased significantly in group II (p < 0.05). Immunohistochemical staining findings showed that RANKL immunoreactivity was stronger in group II than in the other groups. As to OPG immunoreactivity, no difference was found between the groups. Immunohistochemical parameters were higher in group III than in group I, while both were lower than group II. On the basis of these findings, low-level laser irradiation accelerates the bone remodeling process by stimulating osteoblastic and osteoclastic cell proliferation and function during orthodontic tooth movement.
Literature
1.
Glinkowski W, Pokora L (2001) Lasery w therapii (in Polish) (Lasers in theraphy). Laser instruments-Centrum techniki Laserowej, Warsaw
2.
Van Breugel HH, Bar PR (1992) Power density and exposure time of He-Ne laser irradiation are more important than total energy dose in photo-biomodulation of human fibroblasts in vitro. Lasers Surg Med 12:528–537PubMedCrossRef
3.
Schultz RJ (1985) Effects of varying intensities of laser energy on articular cartilage. Lasers Surg Med 5:577–588PubMedCrossRef
4.
Poon VK, Huang L, Burd A (2005) Biostimulation of dermal fibroblast by sublethal Q-switched Nd:YAG 532-nm laser: collagen remodeling and pigmentation. J Photochem Photobiol B 81:1–8PubMedCrossRef
5.
Mohammed IF, Al-Mustawfi N, Kaka LN (2007) Promotion of regenerative processes in injured peripheral nerve induced by low-level laser therapy. Photomed Laser Surg 25:107–111PubMedCrossRef
6.
Maiya GA, Kumar P, Rao L (2005) Effect of low intensity helium-neon (He-Ne) laser irradiation on diabetic wound healing dynamics. Photomed Laser Surg 23:187–190PubMedCrossRef
7.
Nicolau R, Jorgetti V, Rigau J, Pacheco M, Reis LM, Zangaro R (2003) Effect of low-power GaAlAs laser (660 nm) on bone structure and cell activity: an experimental animal study. Lasers Med Sci 18:89–94CrossRef
8.
Turhani D, Scheriau M, Kapral D, Benesch T, Jonke E, Bantleon HP (2006) Pain relief by single low-level laser irradiation in orthodontic patients undergoing fixed appliance therapy. Am J Orthod Dentofacial Orthop 130(3):371–377PubMedCrossRef
9.
Saito S, Shimizu N (1997) Stimulatory effects of low-power laser irradiation on bone regeneration in midpalatal suture during expansion in the rat. Am J Orthod Dentofacial Orthop 111(5):525–532PubMedCrossRef
10.
Kawasaki K, Shimizu NV (2000) Effects of low-energy irradiation on bone remodelling during experimental tooth movement in rats. Lasers Surg Med 26:282–291PubMedCrossRef
11.
Youssef M, Ashkar S, Hamade E, Gutknecht N, Lampert F, Mir M (2008) The effect of low-level laser therapy during orthodontic movement: a preliminary study. Lasers Med Sci 23(1):27–33PubMedCrossRef
12.
Yamaguchi M, Fujita S, Yoshida T, Oikawa K, Utsunomiya T, Yamamoto H, Kasai K (2007) Low-energy laser irradiation stimulates the tooth movement velocity via expression of M-CSF and c-fms. Orthod Waves 66:139–148CrossRef
13.
Simonet WS, Lacey DL, Dunstan CR, Kelley M, Chang MS, Lüthy R, Nguyen HQ, Wooden S, Bennett L, Boone T, Shimamoto G, DeRose M, Elliott R, Colombero A, Tan HL, Trail G, Sullivan J, Davy E, Bucay N, Renshaw-Gegg L, Hughes TM, Hill D, Pattison W, Campbell P, Sander S, Van G, Tarpley J, Derby P, Lee R, Boyle WJ (1997) Osteoprotegerin—a novel secreted protein involved in the regulation of bone density. Cell 89:309–319PubMedCrossRef
14.
Ogasawara T, Yoshimine Y, Kiyoshima T, Kobayashi I, Matsuo K, Akamine A, Sakai H (2004) In situ expression of RANKL, RANK, Osteoprotegerin and cytokines in osteoclasts of rat periodontal tissue. J Periodontal Res 39:42–49PubMedCrossRef
15.
Cruz D, Kohara E, Ribeiro M, Wetter N (2004) Effects of low-intensity laser therapy on the orthodontic movement velocity of human teeth: a preliminary study. Lasers Surg Med 35:117–120PubMedCrossRef
16.
Goulart CS, Nouer PRA, Mouramartins L, Garbin IU, Lizerelli RDZ (2006) Photoradiation and orthodontic movement: experimental study with canines. Photomed Laser Surg 242:192–196CrossRef
17.
Sun X, Zhu X, Xu C, Ye N, Zhu H (2001) Effects of low energy laser on tooth movement and remodeling of alveolar bone in rabbits. Hua Xi Kou Qiang Yi Xue Za Zhi 19(5):290–293PubMed
18.
Limpanichkul W, Godfrey K, Srisuk N, Rattanayatikul C (2006) Effects of low-level laser therapy on the rate of orthodontic tooth movement. Orthod Craniofac Res 9:38–43PubMedCrossRef
19.
Seifi M, Shafeei HA, Daneshdoost S (2007) Effects of two types of low-level laser wave lengths (850 and 630 nm) on the orthodontic tooth movements in rabbits. Lasers Med Sci 22:261–264PubMedCrossRef
20.
Fujita S, Yamaguchi M, Utsunomiya T, Yamamoto H, Kasai K (2008) Low-energy laser stimulates tooth movement velocity via expression of RANK and RANKL. Orthod Craniofac Res 11:143–155PubMedCrossRef
21.
Yoshida T, Yamaguchi M, Utsunomiya T, Kato M, Arai Y, Kaneda T, Yamamoto H, Kasai K (2009) Low-energy laser irradiation accelerates the velocity of tooth movement via stimulation of the alveolar bone remodeling. Orthod Craniofac Res 12(4):289–298PubMedCrossRef
22.
Yamaguchi M, Hayashi M, Fujita S, Yoshida T, Utsunomiya T, Yamamoto H, Kasai K (2010) Low-energy laser irradiation facilitates the velocity of tooth movement and the expressions of matrix metalloproteinase-9, cathepsin K, and alpha(v) beta(3) integrin in rats. Eur J Orthod 32:131–139PubMedCrossRef
23.
Kim YD, Kim SS, Kim SJ, Kwon DW, Jeon ES, Son WS (2010) Low-level laser irradiation facilitates fibronectin and collagen type I turnover during tooth movement in rats. Lasers Med Sci 25:25–31PubMedCrossRef
24.
Hillenkamp F (1990) Interaction between laser radiation and biological systems. In: Hillenkamp F, Pratesi R, Scassi CA (eds) Lasers in biology and medicine. Plenum Press, New York, pp 37–68
25.
Luger EJ, Rochkind S, Wollman Y, Kogan G, Dekel S (1998) Effect of low-power laser irradiation on the mechanical properties of bone fracture healing in rats. Lasers Surg Med 22:97–102PubMedCrossRef
26.
Aihara N, Yamaguchi M, Kasai K (2006) Low-energy laser stimulates formation of osteoclast-like cells via RANK expression in vitro. Lasers Med Sci 21:24–33PubMedCrossRef
27.
Karu T (1999) Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol 49:1–17
28.
Hentunen TA, Cunningham NS, Vuolteenaho O, Reddi AH, Vaananen HK (1994) Osteoclast recruiting activity in bone matrix. Bone Miner 25:183–198PubMedCrossRef
29.
30.
Zaidi M, Pazianas M, Shankar VS, Bax BE, Bax CM, Bevis PJ, Stevens C, Huang CL, Blake DR, Moonga BS (1993) Osteoclast function and its control. Exp Physiol 78:721–739PubMed
31.
Kim YD, Song WW, Kim SS, Kim GC, Hwang DS, Shin SH, Kim UK, Kim JR, Chung IK (2009) Expression of receptor of nuclear factor-kB ligand, receptor activator of nuclear factor-kB, and osteoprotegerin, following low-level laser treatment on deproteinized bovine bone graft in rats. Lasers Med Sci 24(4):577–584PubMedCrossRef
32.
Hamajima S, Hiratsuka K, Kiyama-Kishikawa M, Tagawa T, Kawahara M, Ohta H, Sasahara H, Abiko Y (2003) Effect of low-level laser irradiation on osteoglycin gene expression in osteoblasts. Lasers Med Sci 18:78–82PubMedCrossRef
33.
Shimizu N, Yamaguchi M, Goseki T, Shibata Y, Takiguchi H, Iwasawa T (1995) Inhibition of PGE2 and IL-1β production by low-power laser irradiation in stretched human periodontal ligament cells. J Dent Res 74:1382–1388PubMedCrossRef
34.
Conlan MJ, Rapley JW, Cob CM (1996) Biostimulation of wound healing by low-energy laser irradiation: a review. J Clin Periodontol 23:492–496PubMedCrossRef
Metadata
Title
Metrical and histological investigation of the effects of low-level laser therapy on orthodontic tooth movement
Authors
Burcu Ayse Altan
Oral Sokucu
Mahmud M. Ozkut
Sevinc Inan
Publication date
01-01-2012
Publisher
Springer-Verlag
Published in
Lasers in Medical Science / Issue 1/2012
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-010-0853-2

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