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01-02-2016 | Original Article

An evaluation of the effect of pulsed wave low-level laser therapy on the biomechanical properties of the vertebral body in two experimental osteoporosis rat models

Authors: Mohammad Bayat, Mohammadjavad Fridoni, Hossein Nejati, Atarodalsadat Mostafavinia, Maryam Salimi, Mahdi Ghatrehsamani, Mohammad-amin Abdollahifar, Azam Najar, Saba Bayat, Fatemesadat Rezaei

Published in: Lasers in Medical Science | Issue 2/2016

Abstract

Osteoporosis (OP) increases vertebral fragility as a result of the biomechanical effects of diminished bone structure and composition. This study has aimed to assess the effects of pulsed wave low-level laser therapy (PW LLLT) on cancellous bone strength of an ovariectomized (OVX-d) experimental rat model and a glucocorticoid-induced OP (GIOP) experimental rat model. There were four OVX-d groups and four dexamethasone-treated groups. A group of healthy rats was used for baseline evaluations. The OVX-d rats were further subdivided into the following groups: control rats with OP, OVX-d rats that received alendronate, OVX-d rats treated with PW LLLT, and OVX-d rats treated with alendronate and PW LLLT. The remaining rats received dexamethasone and were divided into four groups: control, alendronate-treated rats, laser-treated rats, and laser-treated rats with concomitant administration of alendronate. PW LLLT (890 nm, 80 Hz, 0.972 J/cm²) was performed on the spinal processes of the T12, L1, L2, and L3 vertebras. We extracted the L1 vertebrae and submitted them to a mechanical compression test. Biomechanical test findings showed positive effects of the PW LLLT and alendronate administration on increasing bending stiffness and maximum force of the osteoporotic bones compared to the healthy group. However, laser treatment of OVA-d rats significantly increased stress high load compared to OVA-d control rats. PW LLLT preserved the cancellous (trabecular) bone of vertebra against the detrimental effects of OV-induced OP on bone strength in rats compared to control OV rats.

Compston J (2009) Clinical and therapeutic aspects of osteoporosis. Eur J Radiol 71:388–391CrossRefPubMed

Fields AJ, Keaveny TM (2012) Trabecular architecture and vertebral fragility in osteoporosis. Curr Osteoporos Rep 10:132–140CrossRefPubMed

Wright NC, Looker AC, Saag KG, Curtis JR, Delzell ES, Randall S, Dawson-Hughes B (2014) The recent prevalence of osteoporosis and low bone mass in the United States based on bone mineral density at the femoral neck or lumbar spine. J Bone Miner Res 29:2520–2526. doi:10.1002/JBMR.2269 CrossRefPubMed

Looker AC, Borrud LG, Dawson-Hughes B, Shepherd JA, Wright NC (2012) Osteoporosis or low bone mass at the femur neck or lumbar spine in older adults: United States, 2005–2008. NCHS Data Brief 93:1–8PubMed

Sattui SE, Saag KG (2014) Fracture mortality: associations with epidemiology and osteoporosis treatment. Nat Rev Endocrinol 10:592–602CrossRefPubMed

Egermann M, Goldhahn J, Schneider E (2005) Animal models for fracture treatment in osteoporosis. Osteoporos Int 16:S129–S138CrossRefPubMed

Peng Z, Tuukkanen J, Zhang H, Jämsä T, Väänänen HK (1994) The mechanical strength of bone in different rat models of experimental osteoporosis. Bone 15:523–532CrossRefPubMed

Turner CH, Burr DB (1993) Basic biomechanical measurements of bone: a tutorial. Bone 14:595–608CrossRefPubMed

Phillips FM, Turner AS, Seim HB III, MacLeay J, Toth CA, Pierce AR, Wheeler DL (2006) In vivo BMP-7 (OP-1) enhancement of osteoporotic vertebral bodies in an ovine model. Spine J 6:500–506CrossRefPubMed

10.

Benhamou CL (2007) Effects of osteoporosis medications on bone quality. Joint Bone Spine 74:39–47CrossRefPubMed

11.

Appelman-Dijkstra NM, Papapoulos SE (2014) Novel approaches to the treatment of osteoporosis. Best Pract Res Clin Endocrinol Metab 28:843–857CrossRefPubMed

12.

Fávaro–Pípi E, Ribeiro DA, Ribeiro JU, Bossini P, Oliveira P, Parizotto NA, Renno ACM (2011) Low-level laser therapy induces differential expression of osteogenic genes during bone repair in rats. Photomed Laser Surg 29:311–317CrossRefPubMed

13.

Kiyosaki T, Mitsui N, Suzuki N, Shimizu N (2010) Low-level laser therapy stimulates mineralization via increased Runx2 expression and ERK phosphorylation in osteoblasts. Photomed Laser Surg 28(S1):167–172CrossRef

14.

Shimizu N, Mayahara K, Kiyosaki T, Yamaguchi A, Ozawa Y, Abiko Y (2007) Low‐intensity laser irradiation stimulates bone nodule formation via insulin‐like growth factor‐I expression in rat calvarial cells. Lasers Surg Med 39:551–559CrossRefPubMed

15.

Luger EJ, Rochkind S, Wollman Y, Kogan G, Dekel S (1998) Effect of low‐power laser irradiation on the mechanical properties of bone fracture healing in rats. Lasers Surg Med 22:97–102CrossRefPubMed

16.

Trelles MA, Mayayo E (1987) Bone fracture consolidates faster with low‐power laser. Lasers Surg Med 7:36–45CrossRefPubMed

17.

Pinheiro ALB, Júnior L, Fde A, Gerbi ME, Ramalho LM, Marzola C, Ponzi EA, Soares AO, Carvalho LC, Lima HC, Goncalves TO (2003) Effect of 830-nm laser light on the repair of bone defects grafted with inorganic bovine bone and decalcified cortical osseus membrane. J Clin Laser Med Surg 21:301–306CrossRefPubMed

18.

Ko CY, Kang H, Ryu Y, Jung B, Kim H, Jeong D, Kim HS (2013) The effects of minimally invasive laser needle system on suppression of trabecular bone loss induced by skeletal unloading. Lasers Med Sci 28:1495–1502CrossRefPubMed

19.

Ko CY, Kang H, Seo DH, Jung B, Schreiber J, Kim HS (2013) Low-level laser therapy using the minimally invasive laser needle system on osteoporotic bone in ovariectomized mice. Med Eng Phys 35:1015–1019CrossRefPubMed

20.

Diniz JS, Nicolau RA, de Melo Ocarino N, do Carmo Magalhães F, de Oliveira Pereira RD, Serakides R (2009) Effect of low-power gallium-aluminum-arsenium laser therapy (830 nm) in combination with bisphosphonate treatment on osteopenic bone structure: an experimental animal study. Lasers Med Sci 24:347–352CrossRefPubMed

21.

Hashmi JT, Huang YY, Sharma SK, Kurup DB, De Taboada L, Carroll JD, Hamblin MR (2010) Effect of pulsing in low‐level light therapy. Lasers Surg Med 42:450–466CrossRefPubMedCentralPubMed

22.

Ueda Y, Shimizu N (2001) Pulse irradiation of low-power laser stimulates bone nodule formation. J Oral Sci 43:55–60CrossRefPubMed

23.

Xu M, Deng T, Mo F, Deng B, Lam W, Deng P, Zhang X, Liu S (2009) Low-intensity pulsed laser irradiation affects RANKL and OPG mRNA expression in rat calvarial cells. Photomed Laser Surg 27:309–315CrossRefPubMed

24.

Ueda Y, Shimizu N (2003) Effects of pulse frequency of low-level laser therapy (LLLT) on bone nodule formation in rat calvarial cells. J Clin Laser Med Surg 21:271–277CrossRefPubMed

25.

Duan J, Na Y, Liu Y, Zhang Y (2012) Effects of the pulse frequency of low-level laser therapy on the tooth movement speed of rat molars. Photomed Laser Surg 30:663–667CrossRefPubMed

26.

Saad A, El Yamany M, Abbas O, Yehia M (2010) Possible role of low level laser therapy on bone turnover in ovariectomized rats. Endocr Regul 44:155–163CrossRefPubMed

27.

Javadieh F, Bayat M, Abdi S, Mohsenifar Z, Razi S (2009) The effects of infrared low-level laser therapy on healing of partial osteotomy of tibia in streptozotocin-induced diabetic rats. Photomed Laser Surg 27:641–646CrossRefPubMed

28.

Saito M, Shiraishi A, Ito M, Sakai S, Hayakawa N, Mihara M, Marumo K (2010) Comparison of effects of alfacalcidol and alendronate on mechanical properties and bone collagen cross-links of callus in the fracture repair rat model. Bone 46:1170–1179CrossRefPubMed

29.

Fridoni M, Masteri Farahani R, Nejati H, Salimi M, Gharavi M, Bayat M, Amini A, Torkman G, Bayat S (2015) Evaluation of the effects of LLLT on biomechanical properties of tibial diaphysis in two rat models of experimental osteoporosis by a three point bending test. Lasers Med Sci 30:1117–1125CrossRefPubMed

30.

Sun P, Cai DH, Li QN, Chen H, Deng WM, He L, Yang L (2010) Effects of alendronate and strontium ranelate on cancellous and cortical bone mass in glucocorticoid-treated adult rats. Calcif Tissue Int 86:495–501CrossRefPubMed

31.

Bayat M, Abdi S, Javadieh F, Mohsenifar Z, Rashid MR (2009) The effects of low-level laser therapy on bone in diabetic and nondiabetic rats. Photomed Laser Surg 27:703–708CrossRefPubMed

32.

Freidouni M, Nejati H, Salimi M, Bayat M, Amini A, Noruzian M, Asgharie MA, Rezaian M (2015) Evaluating glucocorticoid administration on biomechanical properties of rats’ tibial diaphysis. Iran Red Crescent Med J 17:e19389CrossRefPubMedCentralPubMed

33.

Ferretti JL, Gaffuri O, Capozza R, Cointry G, Bozzini C, Olivera M, Zanchetta JR, Bozzini CE (1995) Dexamethasone effects on mechanical, geometric and densitometric properties of rat femur diaphyses as described by peripheral quantitative computerized tomography and bending tests. Bone 16:119–124CrossRefPubMed

34.

Reddy GK (2004) Photobiological basis and clinical role of low-intensity lasers in biology and medicine. J Clin Laser Med Surg 22:141–150CrossRefPubMed

35.

Johnell O, Kanis JA, Oden A, Sernbo I, Redlund-Johnell I, Pitterson C, de Laet B, Jönsson B (2004) Mortality after osteoporotic fractures. Osteoporos Int 15:38–42CrossRefPubMed

36.

Da Silva JA, Jacobs JW, Kirwan JR, Boers M, Saag KG, Inês LB, de Koning EJ, Buttgereit F, Cutolo M, Capell H, Rau R, Bijlsma JW (2006) Safety of low dose glucocorticoid treatment in rheumatoid arthritis: published evidence and prospective trial data. Ann Rheum Dis 65:285–293CrossRefPubMedCentralPubMed

37.

Li M, Shen Y, Halloran BP, Baumann BD, Miller K, Wronski TJ (1996) Skeletal response to corticosteroid deficiency and excess in growing male rats. Bone 19:81–88CrossRefPubMed

38.

Shen V, Birchman R, Liang XG, Wu DD, Lindsay R, Dempster DW (1997) Prednisolone alone, or in combination with estrogen or dietary calcium deficiency or immobilization, inhibits bone formation but does not induce bone loss in mature rats. Bone 21:345–351CrossRefPubMed

39.

Weinstein RS, O’Brien CA, Almeida M, Zhao H, Roberson PK, Jilka RL, Manolagas SC (2011) Osteoprotegerin prevents glucocorticoid-induced osteocyte apoptosis in mice. Endocrinology 152:3323–3331CrossRefPubMedCentralPubMed

40.

Cui L, Li T, Liu Y, Zhou L, Li P, Xu B, Huang L, Chen Y, Liu Y, Tian X, Jee WS, Wu T (2012) Salvianolic acid B prevents bone loss in prednisone-treated rats through stimulation of osteogenesis and bone marrow angiogenesis. PLoS One 7:e34647CrossRefPubMedCentralPubMed

41.

Renno ACM, de Moura FM, dos Santos NSA, Tirico RP, Bossini PS, Parizotto NA (2006) The effects of infrared-830 nm laser on exercised osteopenic rats. Lasers Med Sci 21:202–207CrossRef

42.

Renno ACM, de Moura FM, Dos Santos NSA, Tirico RP, Bossini PS, Parizotto NA (2006) Effects of 830-nm laser, used in two doses, on biomechanical properties of osteopenic rat femora. Photomed Laser Surg 24:202–206CrossRefPubMed

43.

Cummings SR, Melton LJ (2002) Epidemiology and outcomes of osteoporotic fractures. Lancet 359:1761–1767CrossRefPubMed

44.

Xie JH, Xu M, Feng XS (2004) Clinical effects of drugs for treatment and prevention of postmenopausal osteoporosis. J Guangzhou Univ Tradit Chin Med 21:156–160

45.

Bayat M (2014) The necessity for increased attention to pulsed low-level laser therapy. Photomed Laser Surg 32:427–428CrossRefPubMed

Title: An evaluation of the effect of pulsed wave low-level laser therapy on the biomechanical properties of the vertebral body in two experimental osteoporosis rat models
Authors: Mohammad Bayat
Mohammadjavad Fridoni
Hossein Nejati
Atarodalsadat Mostafavinia
Maryam Salimi
Mahdi Ghatrehsamani
Mohammad-amin Abdollahifar
Azam Najar
Saba Bayat
Fatemesadat Rezaei
Publication date: 01-02-2016
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 2/2016
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-015-1842-2

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An evaluation of the effect of pulsed wave low-level laser therapy on the biomechanical properties of the vertebral body in two experimental osteoporosis rat models

Abstract

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Abstract

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