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01-02-2016 | Original Article

Photodynamic inactivation of antibiotic-resistant bacteria and biofilms by hematoporphyrin monomethyl ether

Authors: Chengcheng Liu, Min Hu, Dandan Ma, Jin’e Lei, Jiru Xu

Published in: Lasers in Medical Science | Issue 2/2016

Abstract

The worldwide increase in bacterial antibiotic resistance has led to a search for alternative antibacterial therapies. A promising approach to killing antibiotic-resistant bacteria is photodynamic antimicrobial chemotherapy, which uses light in combination with a photosensitizer to induce a phototoxic reaction. We evaluated the photodynamic inactivation (PDI) efficiency of hematoporphyrin monomethyl ether (HMME) on antibiotic-resistant bacteria and biofilms. HMME exhibited no significant dark toxicity and provided dose-dependent inactivation of antibiotic-resistant bacteria and biofilms. After incubation with 100-μM HMME and irradiation with 72-J cm⁻² white light, 4.19–7.59 log₁₀ reductions in survival were achieved in planktonic suspension. Antibiotic-resistant strains were as susceptible to PDI in biofilms as in planktonic suspensions, but the inactivation of bacterial cells in biofilms was attenuated. In addition, gram-positive bacterial strains and biofilms were more susceptible than gram-negative strains and biofilms to the PDI effect of HMME. Thus, HMME is a promising photosensitizer for the treatment of infectious diseases caused by antibiotic-resistant bacteria, especially gram-positive bacteria.

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Title: Photodynamic inactivation of antibiotic-resistant bacteria and biofilms by hematoporphyrin monomethyl ether
Authors: Chengcheng Liu
Min Hu
Dandan Ma
Jin’e Lei
Jiru Xu
Publication date: 01-02-2016
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 2/2016
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-015-1859-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Photodynamic inactivation of antibiotic-resistant bacteria and biofilms by hematoporphyrin monomethyl ether

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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