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01-03-2012 | Original Article

Effect of laser on TNF-alpha expression in inflamed human gingival tissue

Authors: Snezana Pesevska, Marija Nakova, Icko Gjorgoski, Nikola Angelov, Kiro Ivanovski, Salvador Nares, Sebastiano Andreana

Published in: Lasers in Medical Science | Issue 2/2012

Abstract

This study sought to evaluate the effect of low-level laser treatment combined with scaling and root planing (SRP) on gingival tissue levels of TNF-alpha in subjects with periodontal disease. Eighty gingival papilla biopsy samples were obtained from 60 patients diagnosed with chronic advanced periodontitis; randomly assigned to three treatment groups (n = 20), as well as 20 subjects with no periodontal disease (group A). Group B received SRP on a single quadrant/day for four consecutive days. On day 5, all quadrants were rescaled. Groups C and D received the same treatment as group B plus laser application with the low-level diode laser (630–670 nm, 1.875 J/cm²) for five and ten consecutive days, respectively. Papilla biopsies were obtained from subjects and evaluated by ELISA for levels of TNF-alpha. The values in the control group were 5.2 ± 3.21 pg/mg and baseline values for the examined groups were 46.01 ± 16.69. Significantly decreased level of TNF-alpha for groups C and D was found after treatment, while group B demonstrated reduction of TNF-alpha of 31.34%. The results of this study show suppression of TNF-alpha in gingival tissue after low-level laser treatment as adjunct to SRP. Data may suggest beneficial anti-inflammatory effects of the laser treatment when used as adjunctive periodontal treatment.

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Title: Effect of laser on TNF-alpha expression in inflamed human gingival tissue
Authors: Snezana Pesevska
Marija Nakova
Icko Gjorgoski
Nikola Angelov
Kiro Ivanovski
Salvador Nares
Sebastiano Andreana
Publication date: 01-03-2012
Publisher: Springer-Verlag
Published in: Lasers in Medical Science / Issue 2/2012
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-011-0898-x

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Effect of laser on TNF-alpha expression in inflamed human gingival tissue

Abstract

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Abstract

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