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Lasers in Medical Science
Published in: Lasers in Medical Science 2/2008

01-04-2008 | Original Article

Effects of laser irradiation on the release of basic fibroblast growth factor (bFGF), insulin like growth factor-1 (IGF-1), and receptor of IGF-1 (IGFBP3) from gingival fibroblasts

Authors: Isil Saygun, Seniz Karacay, Muhittin Serdar, Ali Ugur Ural, Metin Sencimen, Bulent Kurtis

Published in: Lasers in Medical Science | Issue 2/2008

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Abstract

Various studies have shown biostimulation effects of laser irradiation by producing metabolic changes within the cells. Little is known about the biological effect of laser irradiation on the oral tissues. Among the many physiological effects, it is important to recognize that low-level laser therapy (LLLT) may affect release of growth factors from fibroblasts. Therefore, the aim of the present study was to determine whether the laser irradiation can enhance the release of basic fibroblast growth factor (bFGF), insulin-like growth factor-1 (IGF-1), and receptor of IGF-1 (IGFBP3) from human gingival fibroblasts (HGF). The number of all samples in the study were 30, and the samples were randomly divided into three equal groups; In the first group (single dose group), HGF were irradiated with laser energy of 685 nm, for 140 s, 2 J/cm2 for one time, and in the second group, energy at the same dose was applied for two consecutive days (double dose group). The third group served as nonirradiated control group. Proliferation, viability, and bFGF, IGF-1, IGFBP3 analysis of control and irradiated cultures were compared with each other. Both of the irradiated groups revealed higher proliferation and viability in comparison to the control group. Comparison of the single-dose group with the control group revealed statistically significant increases in bFGF (p < 0.01) and IGF-1 (p < 0.01), but IGFBP3 increased insignificantly (p > 0.05). When the double dose group was compared with the control group, significant increases were determined in all of the parameters (p < 0.01). In the comparison of the differences between the two irradiated groups (one dose and two doses), none of the parameters displayed any statistically significant difference (p > 0.05). In both of the laser groups, LLLT increased the cell proliferation and cell viability. The results of this study showed that LLLT increased the proliferation of HGF cells and release of bFGF, IGF-1, and IGFBP3 from these cells. LLLT may play an important role in periodontal wound healing and regeneration by enhancing the production of the growth factors.
Literature
1.
Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K (2005) Determining optimal dose of laser therapy for attachment and proliferation of human oral fibroblasts cultured on titanium implant material. J Biomed Mater Res 73:55–62CrossRef
2.
Mester E, Spiry T, Szende B et al (1971) Effects of laser-ray on wound healing. Am J Surg 122:532–535PubMedCrossRef
3.
Hall G, Anneroth G, Schennings T, Zetterqvist L, Ryden H (1994) Effect of low level laser irradiation on wound healing. An experimental study in rats. Swed Dent J 18:29–34PubMed
4.
Tolstykh PI, Getrsen AV, Eliseenko VI, Sarasek IK (1991) Stimulation of the healing of aseptic wounds using laser irradiation. Khirurgiia Mosk 7:36–40PubMed
5.
Saito S, Shimizn N (1997) Stimulatory effects of low-power laser irradiation on bone regeneration in midpalatal suture during expansion in the rat. Am J Orthod 1111:525–532
6.
Ueda Y, Shimizu N (2001) Pulse irradiation of low-power laser stimulates bone nodule formation. J Oral Sci 43:555–560
7.
Khadra M, Kasem N, Ellingsen JE, Haanes HR, Lyngstadaas SP (2004) Enhancement of bone formation in rat calvarial bone defects using low level laser therapy. Oral Surg Oral Med Oral Pathol Oral Radiol Endo 97:693–700CrossRef
8.
Clokie C, Bentley KC, Head TW (1991) The effects of the helium neon laser on postsurgical discomfort: a pilot study. J Can Dent Assoc 57:584–586PubMed
9.
Eckerdal A, Lehmann Bastian H (1996) Can low reactive level laser therapy be used in the treatment of neurogenic facial pain? A double blind placebo controlled investigation of patients with trigeminal neuralgia. Laser Ther 8:247–252
10.
Honmura A et al (1992) Therapeutic effect of GaAlAs diode laser irradiation on experimentally induced inflammation in rats. Laser Surg Med 12:441–449CrossRef
11.
Freitas AC, Pinheiro A, Miranda P et al (2001) Assesment of anti-inflammatory effects of 830 nm laser light using C-reactive protein levels. Braz Dent J 12:187–190PubMed
12.
Ohshiro T, Fujino T (1993) Laser applications in plastic and reconstructive surgery. Keio J Med 42:191–195PubMed
13.
Nanami T, Shiba H, Ikeuchi S, Nagai T, Asanami S, Shibata T (1993) Clinical applications and basic studies of laser in dentistry and oral surgery. Keio J Med 42:199–201PubMed
14.
Rochkind S, Nissan M, Razon N, Schwardz M, Bartal A (1986) Electrophysical effect in HeNe laser on normal and injured sciatic nerve in the rat. Acta Neurochirurgica 83:125–130PubMedCrossRef
15.
Kovacs IB, Mester E, Gorog P (1974) Laser induced stimulation of vascularization of the healing wound-ear chamber. Seperatum Experientia 30:341–343CrossRef
16.
Terranova VP, Wikesjö UME (1987) Extracellular matrices and polypeptide growth factors as mediators of functions of cells of the periodontium. A review. J Periodontol 58:371–380PubMed
17.
Wahl SM, Cately CL (1983) Modulation of fibroblast growth by a lymphokine of human T-cell and continuous T cell line origin. J Immunol 130:1226–1230PubMed
18.
Aggarwal BB, Samanta A, Feldman M (2003) Cytokine, vol II. Chapter 31. In: Ensoli B, Sgadari C, Barillari G, Monini P (eds) The fibroblast growth factors. Academic, San Diego pp 747–781
19.
Baired A, Walicke PA (1989) Fibroblast growth factors. Br Med Bull 45:438–452
20.
Gospodarowicz D (1990) Fibroblast growth factors. Chemical structure and biological function. Clin Orthop 257:231–248PubMed
21.
Davidson JM, Broadly KN (1991) Manipulation of the wound healing process with basic fibroblast growth factor. Ann NY Acad Sci 638:306–315PubMedCrossRef
22.
Yu W, Naim JO, Lanzafame RJ (1994) The effects of laser irradiation on the release of bFGF from 3T3 fibroblasts. Photochem Photobiol 59:167–170PubMedCrossRef
23.
Matsuda N, Lin WL, Kumar NM, Cho MI, Genco RJ (1992) Mitogenic, chemotactic and synthetic responses of rat periodontal ligament fibroblastic cells to polypeptide growth factors in vitro. J Periodontol 63:515–525PubMed
24.
25.
26.
Blom S, Holmstrup P, Dabelsteen E (1992) The effect of insulin-like growth factor 1 and human growth hormone on periodontal ligament fibroblast morphology, growth pattern, DNA synthesis and receptor binding. J Periodontal 63:960–968
27.
Aggarwal BB, Samanta A, Feldman M (2003) Cytokine, vol I. Chapter 5. In: Welniak LA, Murphy WJ (eds) Growth hormone. Academic, San Diago, pp 103–113
28.
Somerman MJ, Archer SY, Imm GR et al (1988) A comparative study of human periodontal ligament cells and gingival fibroblasts in vitro. J Dent Res 67:156–159
29.
30.
31.
Giannobile WV (1996) The potential role of growth and differentiation factors in periodontal regeneration. J Periodontol 67:543–553
32.
Lynch SE (2001) Current techniques and concepts in periodontal therapy. In: Polson AM (ed) The role of growth factors in periodontal repair and regeneration, Alan R. Liss, New York, USA, pp 179–198
33.
Kasasa S, Soory M (1995) The influence of insulin-like growth factor (IGF) on C19 steroid conversions by human gingiva and in cultured fibroblasts. J Periodontol 66:966–972PubMed
34.
Loevshall H, Arenholt-Bindslev D (1994) Effect of low level diode laser irradiation of human oral mucosa fibroblast in vitro. Lasers Surg Med 14:347–354CrossRef
35.
Hans HFI, Breugel V, Bar D (1992) Power density and exposure time of He–Ne laser irradiation are more important than total energy dose in photo-biomodulation of human fibroblast in vitro. Laser Surg Med 12:528–537CrossRef
36.
Shields TDS, O’Kane S, Leckey D (1992) The effect of laser irradiation upon human mononuclear leukocytes in vitro. Laser Surg Med 4:11
Metadata
Title
Effects of laser irradiation on the release of basic fibroblast growth factor (bFGF), insulin like growth factor-1 (IGF-1), and receptor of IGF-1 (IGFBP3) from gingival fibroblasts
Authors
Isil Saygun
Seniz Karacay
Muhittin Serdar
Ali Ugur Ural
Metin Sencimen
Bulent Kurtis
Publication date
01-04-2008
Publisher
Springer-Verlag
Published in
Lasers in Medical Science / Issue 2/2008
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-007-0477-3

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