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Archives of Virology
Published in: Archives of Virology 8/2020

Open Access 01-08-2020 | Dengue Virus | Original Article

Cell fusing agent virus (Flavivirus) infection in Aedes aegypti in Texas: seasonality, comparison by trap type, and individual viral loads

Authors: Estelle Martin, Wendy Tang, Cierra Briggs, Helena Hopson, Jose G. Juarez, Selene M. Garcia-Luna, Megan Wise de Valdez, Ismael E. Badillo-Vargas, Monica K. Borucki, Matthias Frank, Gabriel L. Hamer

Published in: Archives of Virology | Issue 8/2020

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Abstract

South Texas has experienced local transmission of Zika virus and of other mosquito-borne viruses such as chikungunya virus and dengue virus in the last decades. Using a mosquito surveillance program in the Lower Rio Grande Valley (LRGV) and San Antonio, TX, from 2016 to 2018, we detected the presence of an insect-specific virus, cell fusing agent virus (CFAV), in the Aedes aegypti mosquito population. We tested 6,326 females and 1,249 males from the LRGV and 659 females from San Antonio for CFAV by RT-PCR using specific primers. Infection rates varied from 0 to 261 per 1,000 mosquitoes in the LRGV and 115 to 208 per 1,000 in San Antonio depending on the month of collection. Infection rates per 1,000 individuals appeared higher in females collected from BG Sentinel 2 traps compared to Autocidal Gravid Ovitraps, but the ratio of the percentage of infected pools did not differ by trap type. The natural viral load in individual males ranged from 1.25 x 102 to 5.50 x 106 RNA copies and in unfed females from 5.42 x 103 to 8.70 x 106 RNA copies. Gravid females were found to harbor fewer viral particles than males and unfed females.
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Metadata
Title
Cell fusing agent virus (Flavivirus) infection in Aedes aegypti in Texas: seasonality, comparison by trap type, and individual viral loads
Authors
Estelle Martin
Wendy Tang
Cierra Briggs
Helena Hopson
Jose G. Juarez
Selene M. Garcia-Luna
Megan Wise de Valdez
Ismael E. Badillo-Vargas
Monica K. Borucki
Matthias Frank
Gabriel L. Hamer
Publication date
01-08-2020
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 8/2020
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-020-04652-0

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