Published in:
01-06-2014 | Brief Report
Genetic variation analysis of apple chlorotic leaf spot virus coat protein reveals a new phylogenetic type and two recombinants in China
Authors:
Shanyi Chen, Ying Zhou, Ting Ye, Lu Hao, Liyun Guo, Zaifeng Fan, Shifang Li, Tao Zhou
Published in:
Archives of Virology
|
Issue 6/2014
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Abstract
In this study, we generated sequences of the apple chlorotic leaf spot virus (ACLSV) coat protein (CP) gene. Genetic variation and phylogenetic analyses were carried out on these sequences along with others reported previously. ACLSV populations clustered into four types: in three of the four types, combinations of three amino acids at positions of 40, 75 and 79 were conserved. The fourth phylogenetic type, newly identified here, was characterized by co-variation of Ser40-Tyr75-Ser79. Statistically significant genetic differentiation and infrequent gene flow were detected among the four types. Two natural recombinants were detected for the first time among ACLSV isolates/genotypes from China.