Skip to main content
Top
Published in: Archives of Virology 6/2014

01-06-2014 | Brief Report

Genetic variation analysis of apple chlorotic leaf spot virus coat protein reveals a new phylogenetic type and two recombinants in China

Authors: Shanyi Chen, Ying Zhou, Ting Ye, Lu Hao, Liyun Guo, Zaifeng Fan, Shifang Li, Tao Zhou

Published in: Archives of Virology | Issue 6/2014

Login to get access

Abstract

In this study, we generated sequences of the apple chlorotic leaf spot virus (ACLSV) coat protein (CP) gene. Genetic variation and phylogenetic analyses were carried out on these sequences along with others reported previously. ACLSV populations clustered into four types: in three of the four types, combinations of three amino acids at positions of 40, 75 and 79 were conserved. The fourth phylogenetic type, newly identified here, was characterized by co-variation of Ser40-Tyr75-Ser79. Statistically significant genetic differentiation and infrequent gene flow were detected among the four types. Two natural recombinants were detected for the first time among ACLSV isolates/genotypes from China.
Appendix
Available only for authorised users
Literature
1.
go back to reference Al Rwahnih M, Turturo C, Minafra A, Saldarelli P, Myrta A, Pallás V, Savino V (2004) Molecular variability of Apple chlorotic leaf spot virus in different hosts and geographical regions. J Plant Pathol 86:117–122 Al Rwahnih M, Turturo C, Minafra A, Saldarelli P, Myrta A, Pallás V, Savino V (2004) Molecular variability of Apple chlorotic leaf spot virus in different hosts and geographical regions. J Plant Pathol 86:117–122
2.
go back to reference Cai Y, Xiang BC, Xi DH, Liu SX, Du YJ (2005) Cloning and prokaryotic expression of CP gene of Apple chlorotic leaf spot virus Kuerle isolate and preparation of its specific antiserum. J Agric Biotechnol 13:533–537 (in Chinese) Cai Y, Xiang BC, Xi DH, Liu SX, Du YJ (2005) Cloning and prokaryotic expression of CP gene of Apple chlorotic leaf spot virus Kuerle isolate and preparation of its specific antiserum. J Agric Biotechnol 13:533–537 (in Chinese)
3.
go back to reference Candresse T, Lanneau M, Revers F, Macquaire G, German S, Grasseau N, Dunez J, Malinowski T (1995) An immunocapture PCR assay adapted to the detection and the analysis of the molecular variability of the Apple chlorotic leaf spot virus. Acta Hortic 386:136–147 Candresse T, Lanneau M, Revers F, Macquaire G, German S, Grasseau N, Dunez J, Malinowski T (1995) An immunocapture PCR assay adapted to the detection and the analysis of the molecular variability of the Apple chlorotic leaf spot virus. Acta Hortic 386:136–147
4.
go back to reference Cieślińska M, Malinowski T, Zawadzka BJ (1995) Studies on several strains of Apple chlorotic leaf spot virus (ACLSV) isolated from different fruit tree species. Acta Hortic 386:63–71 Cieślińska M, Malinowski T, Zawadzka BJ (1995) Studies on several strains of Apple chlorotic leaf spot virus (ACLSV) isolated from different fruit tree species. Acta Hortic 386:63–71
6.
go back to reference Desvignes JC, Boyé R (1989) Different diseases caused by the chlorotic leaf spot virus on the fruit trees. Acta Hortic 235:31–38 Desvignes JC, Boyé R (1989) Different diseases caused by the chlorotic leaf spot virus on the fruit trees. Acta Hortic 235:31–38
7.
go back to reference Dunez J, Marenaud G, Delbos RP, Lansac M (1972) Variability of symptoms induced by the Apple chlorotic leaf spot (CLSV). A type of CLSV probably responsible for bark split disease of prune trees. Plant Dis Rep 56:293–295 Dunez J, Marenaud G, Delbos RP, Lansac M (1972) Variability of symptoms induced by the Apple chlorotic leaf spot (CLSV). A type of CLSV probably responsible for bark split disease of prune trees. Plant Dis Rep 56:293–295
8.
go back to reference German-Retana S, Bergey B, Delbos RP, Candresse T, Dunez J (1997) Complete nucleotide sequence of the genome of a severe cherry isolate of apple chlorotic leaf spot trichovirus (ACLSV). Arch Virol 142:833–841PubMedCrossRef German-Retana S, Bergey B, Delbos RP, Candresse T, Dunez J (1997) Complete nucleotide sequence of the genome of a severe cherry isolate of apple chlorotic leaf spot trichovirus (ACLSV). Arch Virol 142:833–841PubMedCrossRef
9.
go back to reference German S, Candresse T, Lanneau M, Huet JC, Pernollet JC, Dunez J (1990) Nucleotide sequence and genomic organization of Apple chlorotic leaf spot closterovirus. Virology 179:104–112PubMedCrossRef German S, Candresse T, Lanneau M, Huet JC, Pernollet JC, Dunez J (1990) Nucleotide sequence and genomic organization of Apple chlorotic leaf spot closterovirus. Virology 179:104–112PubMedCrossRef
10.
go back to reference German S, Delbos RP, Candresse T, Lanneau M, Dunez J (1997) Complete nucleotide sequence of the genome of a severe cherry isolate of Apple chlorotic leaf spot trichovirus (ACLSV). Arch Virol 142:833–841CrossRef German S, Delbos RP, Candresse T, Lanneau M, Dunez J (1997) Complete nucleotide sequence of the genome of a severe cherry isolate of Apple chlorotic leaf spot trichovirus (ACLSV). Arch Virol 142:833–841CrossRef
12.
go back to reference Hudson RR, Boos DD, Kaplan NL (1992) A statistical test for detecting geographic subdivision. Mol Biol Evol 9:138–151PubMed Hudson RR, Boos DD, Kaplan NL (1992) A statistical test for detecting geographic subdivision. Mol Biol Evol 9:138–151PubMed
13.
go back to reference Jelkmann W (1996) The nucleotide sequence of a strain of Apple chlorotic leaf spot virus (ACLSV) responsible for plum pseudopox and its relation to an apple and plum bark split strain. Phytopathology 86:101 Jelkmann W (1996) The nucleotide sequence of a strain of Apple chlorotic leaf spot virus (ACLSV) responsible for plum pseudopox and its relation to an apple and plum bark split strain. Phytopathology 86:101
14.
go back to reference Jelkmann W, Kunze L (1995) Plum pseudopox in German prune after infection with an isolate of Apple chlorotic leafspot virus causing plum line pattern. Acta Hortic 386:122–125 Jelkmann W, Kunze L (1995) Plum pseudopox in German prune after infection with an isolate of Apple chlorotic leafspot virus causing plum line pattern. Acta Hortic 386:122–125
15.
go back to reference King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (2012) Virus taxonomy: classification and nomenclature of viruses: ninth report of the international committee on taxonomy of viruses. Elsevier, San Diego King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (2012) Virus taxonomy: classification and nomenclature of viruses: ninth report of the international committee on taxonomy of viruses. Elsevier, San Diego
16.
go back to reference Krizbai L, Ember I, Nemeth M, Kôlber M, Pasquini G, Faggioli F (2001) Characterization of Hungarian isolates of Apple chlorotic leafspot virus. Acta Hortic 1:291–306 Krizbai L, Ember I, Nemeth M, Kôlber M, Pasquini G, Faggioli F (2001) Characterization of Hungarian isolates of Apple chlorotic leafspot virus. Acta Hortic 1:291–306
17.
go back to reference Liu FC, Wang HY (1989) On latent viruses of apple in China II: indexing of latent viruses in apple scion varieties and dwarf rootstocks. Acta Phytopathol Sinica 19:193–197 (in Chinese) Liu FC, Wang HY (1989) On latent viruses of apple in China II: indexing of latent viruses in apple scion varieties and dwarf rootstocks. Acta Phytopathol Sinica 19:193–197 (in Chinese)
18.
go back to reference Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452PubMedCrossRef Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452PubMedCrossRef
19.
go back to reference Lemey P, Salemi M, Vandamme A (2009) The phylogenetic handbook: a practical approach to phylogenetic analysis and hypothesis testing, 2nd edn. Cambridge University Press, CambridgeCrossRef Lemey P, Salemi M, Vandamme A (2009) The phylogenetic handbook: a practical approach to phylogenetic analysis and hypothesis testing, 2nd edn. Cambridge University Press, CambridgeCrossRef
20.
go back to reference Magome H, Yoshikawa N, Takahashi T, Ito T, Miyakawa T (1997) Molecular variability of the genomes of capilloviruses from apple, Japanese pear, European pear, and citrus trees. Phytopathology 87:389–396PubMedCrossRef Magome H, Yoshikawa N, Takahashi T, Ito T, Miyakawa T (1997) Molecular variability of the genomes of capilloviruses from apple, Japanese pear, European pear, and citrus trees. Phytopathology 87:389–396PubMedCrossRef
21.
go back to reference Marini DB, Gibson PG, Scott SW (2008) The complete nucleotide sequence of an isolate of apple chlorotic leaf spot virus from peach (Prunus persica (L.) Batch). Arch Virol 153:1003–1005PubMedCrossRef Marini DB, Gibson PG, Scott SW (2008) The complete nucleotide sequence of an isolate of apple chlorotic leaf spot virus from peach (Prunus persica (L.) Batch). Arch Virol 153:1003–1005PubMedCrossRef
22.
go back to reference Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P (2010) RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26:2462–2463PubMedCentralPubMedCrossRef Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P (2010) RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26:2462–2463PubMedCentralPubMedCrossRef
23.
go back to reference Nagy PD (2008) Recombination in plant RNA viruses. In: Roossinck M (ed) Plant virus evolution. Springer, Berlin, pp 133–156CrossRef Nagy PD (2008) Recombination in plant RNA viruses. In: Roossinck M (ed) Plant virus evolution. Springer, Berlin, pp 133–156CrossRef
24.
go back to reference Németh M (1986) Viruses, mycoplasma and rickettsia diseases of fruit trees. Akadémiai Kiadó, Budapest Németh M (1986) Viruses, mycoplasma and rickettsia diseases of fruit trees. Akadémiai Kiadó, Budapest
25.
go back to reference Niu FQ, Pan S, Wu ZJ, Jiang DM, Li SF (2012) Complete nucleotide sequences of the genomes of two isolates of apple chlorotic leaf spot virus from peach (Prunus persica) in China. Arch Virol 157:783–786PubMedCrossRef Niu FQ, Pan S, Wu ZJ, Jiang DM, Li SF (2012) Complete nucleotide sequences of the genomes of two isolates of apple chlorotic leaf spot virus from peach (Prunus persica) in China. Arch Virol 157:783–786PubMedCrossRef
26.
go back to reference Pasquini G, Faggioli F, Pilotti M, Lumia V, Barba M (1998) Characterisation of Apple chlorotic leaf spot virus isolates from Italy. Acta Hortic 472:195–199 Pasquini G, Faggioli F, Pilotti M, Lumia V, Barba M (1998) Characterisation of Apple chlorotic leaf spot virus isolates from Italy. Acta Hortic 472:195–199
27.
go back to reference Rana T, Chandel V, Kumar Y, Ram R, Hallan V, Zaidi AA (2010) Molecular variability analyses of Apple chlorotic leaf spot virus capsid protein. J Biosci 35:605–615PubMedCrossRef Rana T, Chandel V, Kumar Y, Ram R, Hallan V, Zaidi AA (2010) Molecular variability analyses of Apple chlorotic leaf spot virus capsid protein. J Biosci 35:605–615PubMedCrossRef
28.
go back to reference Sato K, Yoshikawa N, Takahashi T (1993) Complete nucleotide sequence of the genome of an apple isolate of Apple chlorotic leaf spot virus. J Gen Virol 74:1927–1931PubMedCrossRef Sato K, Yoshikawa N, Takahashi T (1993) Complete nucleotide sequence of the genome of an apple isolate of Apple chlorotic leaf spot virus. J Gen Virol 74:1927–1931PubMedCrossRef
29.
go back to reference Song YS, Hong N, Wang LP, Hu HJ, Tian R, Xu WX, Ding F, Wang GP (2011) Molecular and serological diversity in Apple chlorotic leaf spot virus from sand pear (Pyrus pyrifolia) in China. Eur J Plant Pathol 130:183–196CrossRef Song YS, Hong N, Wang LP, Hu HJ, Tian R, Xu WX, Ding F, Wang GP (2011) Molecular and serological diversity in Apple chlorotic leaf spot virus from sand pear (Pyrus pyrifolia) in China. Eur J Plant Pathol 130:183–196CrossRef
30.
go back to reference Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCentralPubMedCrossRef Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCentralPubMedCrossRef
31.
go back to reference Wu ZB, Ku HM, Chen YK, Chang CJ, Jan FJ (2010) Biological and molecular characterization of Apple chlorotic leaf spot virus causing chlorotic leaf spot on pear (Pyrus pyrifolia) in Taiwan. HortScience 45:1073–1078 Wu ZB, Ku HM, Chen YK, Chang CJ, Jan FJ (2010) Biological and molecular characterization of Apple chlorotic leaf spot virus causing chlorotic leaf spot on pear (Pyrus pyrifolia) in Taiwan. HortScience 45:1073–1078
32.
go back to reference Yaegashi H, Isogai M, Tajima H, Sano T, Yoshikawa N (2007) Combinations of two amino acids (Ala40 and Phe75 or Ser40 and Tyr75) in the coat protein of apple chlorotic leaf spot virus are crucial for infectivity. J Gen Virol 88:2611–2618PubMedCrossRef Yaegashi H, Isogai M, Tajima H, Sano T, Yoshikawa N (2007) Combinations of two amino acids (Ala40 and Phe75 or Ser40 and Tyr75) in the coat protein of apple chlorotic leaf spot virus are crucial for infectivity. J Gen Virol 88:2611–2618PubMedCrossRef
33.
go back to reference Yoshikawa N, Takahashi T (1988) Properties of RNAs and proteins of Apple stem grooving and Apple chlorotic leaf spot viruses. J Gen Virol 88:2611–2618 Yoshikawa N, Takahashi T (1988) Properties of RNAs and proteins of Apple stem grooving and Apple chlorotic leaf spot viruses. J Gen Virol 88:2611–2618
34.
go back to reference Zheng YY, Wang GP, Hong N, Song YS, You H (2007) Partial molecular characterization of Apple chlorotic leaf spot virus from peach and apple trees and prokaryotic expression for cp gene. Acta Phytopathol Sinica 37:356–361 (in Chinese) Zheng YY, Wang GP, Hong N, Song YS, You H (2007) Partial molecular characterization of Apple chlorotic leaf spot virus from peach and apple trees and prokaryotic expression for cp gene. Acta Phytopathol Sinica 37:356–361 (in Chinese)
Metadata
Title
Genetic variation analysis of apple chlorotic leaf spot virus coat protein reveals a new phylogenetic type and two recombinants in China
Authors
Shanyi Chen
Ying Zhou
Ting Ye
Lu Hao
Liyun Guo
Zaifeng Fan
Shifang Li
Tao Zhou
Publication date
01-06-2014
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 6/2014
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-013-1927-9

Other articles of this Issue 6/2014

Archives of Virology 6/2014 Go to the issue
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.