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Archives of Virology
Published in: Archives of Virology 6/2012

01-06-2012 | Original Article

Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea

Authors: Go Atsumi, Kenji S. Nakahara, Tomoko Sugikawa Wada, Sun Hee Choi, Chikara Masuta, Ichiro Uyeda

Published in: Archives of Virology | Issue 6/2012

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Abstract

Many plant viruses encode suppressors of RNA silencing, including the helper component-proteinase (HC-Pro) of potyviruses. Our previous studies showed that a D-to-Y mutation at amino acid position 193 in HC-Pro (HC-Pro-D193Y) drastically attenuated the virulence of clover yellow vein virus (ClYVV) in legume plants. Furthermore, RNA-silencing suppression (RSS) activity of HC-Pro-D193Y was significantly reduced in Nicotiana benthamiana. Here, we examine the effect of expression of heterologous suppressors of RNA silencing, i.e., tomato bushy stunt virus p19, cucumber mosaic virus 2b, and their mutants, on the virulence of the ClYVV point mutant with D193Y (Cl-D193Y) in pea. P19 and 2b fully and partially complemented Cl-D193Y multiplication and virulence, including lethal systemic HR in pea, respectively, but the P19 and 2b mutants with defects in their RSS activity did not. Our findings strongly suggest that the D193Y mutation exclusively affects RSS activity of HC-Pro and that RSS activity is necessary for ClYVV multiplication and virulence in pea.
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Metadata
Title
Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea
Authors
Go Atsumi
Kenji S. Nakahara
Tomoko Sugikawa Wada
Sun Hee Choi
Chikara Masuta
Ichiro Uyeda
Publication date
01-06-2012
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 6/2012
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-012-1281-3

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