Top

Published in:

01-06-2010 | Review Article

The dextran sulphate sodium (DSS) model of colitis: an overview

Authors: Livingstone Solomon, Sorsiah Mansor, Peter Mallon, Eilish Donnelly, Margaret Hoper, Morris Loughrey, Stephen Kirk, Keith Gardiner

Published in: Comparative Clinical Pathology | Issue 3/2010

Abstract

The dextran sulphate sodium model of colitis has demonstrated several correlations with human inflammatory bowel disease and is deemed suitable for investigating pathogenesis, therapeutic options and the dysplasia–adenocarcinoma sequence of inflammatory bowel disease. It is widely applicable to mice, rats, hamsters and guinea pigs. This review explores the features of this model and identifies areas for further research studies.

Axelsson LG, Landstrom E, Goldschmidt TJ, Gronberg A, Bylund-Fellenius AC (1996) Dextran sulfate sodium (dss) induced experimental colitis in immunodeficient mice: effects in cd4(+) -cell depleted, athymic and nk-cell depleted scid mice. Inflamm Res 45:181–191CrossRefPubMed

Axelsson L, Landstrom E, Bylund-Fellenius A (1998) Experimental colitis induced by dextran sulphate sodium in mice: beneficial effects of sulphasalazine and olsalazine. Aliment Pharmacol Ther 12:925CrossRefPubMed

Bailey RW, Bourne EJ (1961) Intracellular glycosidases of dextran-producing bacteria. Nature 191:277–278CrossRefPubMed

Breider MA, Eppinger M, Gough A (1997) Intercellular adhesion molecule-1 expression in dextran sodium sulfate-induced colitis in rats. Vet Pathol 34:598–604CrossRefPubMed

Chen Y, Chou K, Fuchs E, Havran WL, Boismenu R (2002) Protection of the intestinal mucosa by intraepithelial gamma delta t cells. Proc Natl Acad Sci U S A 99:14338–14343CrossRefPubMed

Chiba T (1993) cell kinetics of carcinoma originating from rat colitis induced by dextran sulphate sodium. Nippon Shokakibyo Gakkai Zasshi 90:774–781PubMed

Clapper ML, Cooper HS, Chang WC (2007) Dextran sulfate sodium-induced colitis-associated neoplasia: a promising model for the development of chemopreventive interventions. Acta Pharmacol Sin 28:1450–1459CrossRefPubMed

Cooper HS, Murthy SN, Shah RS, Sedergran DJ (1993) Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest 69:238–249PubMed

Cooper HS, Murthy S, Kido K, Yoshitake H, Flanigan A (2000) Dysplasia and cancer in the dextran sulfate sodium mouse colitis model. Relevance to colitis-associated neoplasia in the human: a study of histopathology, b-catenin and p53 expression and the role of inflammation. Carcinogenesis 21:757–768CrossRefPubMed

Dieleman LA, Palmen MJ, Akol H, Bloemena E, Pena AS, Meuwissen SG, Van Rees EP (1998) Chronic experimental colitis induced by dextran sulphate sodium (dss) is characterized by th1 and th2 cytokines. Clin Exp Immunol 114:385–391CrossRefPubMed

Egger B, Bajaj-Elliott M, MacDonald TT, Inglin R, Eysselein VE, Buchler MW (2000) Characterisation of acute murine dextran sodium sulphate colitis: cytokine profile and dose dependency. Digestion 62:240–248CrossRefPubMed

Elson CO, Sartor RB, Tennyson GS, Riddell RH (1995) Experimental models of inflammatory bowel disease. Gastroenterology 109:1344–1367CrossRefPubMed

Elson C, Cong Y, Brandwein S et al (1998) Experimental models to study molecular mechanisms underlying intestinal inflammation. Ann N Y Acad Sci 859:85–95CrossRefPubMed

Gaudio E, Taddei G, Vetuschi A, Sferra R, Frieri G, Ricciardi G, Caprilli R (1999) Dextran sulfate sodium (dss) colitis in rats: clinical, structural, and ultrastructural aspects. Dig Dis Sci 44:1458–1475CrossRefPubMed

Geier MS, Smith CL, Butler RN, Howarth GS (2008) Small-intestinal manifestations of dextran sulfate sodium consumption in rats and assessment of the effects of lactobacillus fermentum br11. Dig Dis Sci 54:1222–1228CrossRefPubMed

Hans W, Scholmerich J, Gross V, Falk W (2000) The role of the resident intestinal flora in acute and chronic dextran sulfate sodium-induced colitis in mice. Eur J Gastroenterol Hepatol 12:267–273CrossRefPubMed

Iwanaga T, Hoshi O, Han H, Fujita T (1994) Morphological analysis of acute ulcerative colitis experimentally induced by dextran sulfate sodium in the guinea pig: some possible mechanisms of cecal ulceration. J Gastroenterol 29:430–438CrossRefPubMed

Kitajima S, Takuma S, Morimoto M (1999a) Changes in colonic mucosal permeability in mouse colitis induced with dextran sulfate sodium. Exp Anim 48:137–143CrossRefPubMed

Kitajima S, Takuma S, Morimoto M (1999b) Tissue distribution of dextran sulfate sodium (dss) in the acute phase of murine dss-induced colitis. J Vet Med Sci 61:67–70CrossRefPubMed

Kitajima S, Morimoto M, Sagara E, Shimizu C, Ikeda Y (2001) Dextran sodium sulfate-induced colitis in germ-free iqi/jic mice. Exp Anim 50:387–395CrossRefPubMed

Kullmann F, Messmann H, Alt M, Gross V, Bocker T, Scholmerich J, Ruschoff J (2001) Clinical and histopathological features of dextran sulfate sodium induced acute and chronic colitis associated with dysplasia in rats. Int J Colorectal Dis 16:238–246CrossRefPubMed

Mahler M, Bristol IJ, Leiter EH, Workman AE, Birkenmeier EH, Elson CO, Sundberg JP (1998) Differential susceptibility of inbred mouse strains to dextran sulfate sodium-induced colitis. Am J Physiol 274:G544–551PubMed

Mahler M, Bristol IJ, Sundberg JP, Churchill GA, Birkenmeier EH, Elson CO, Leiter EH (1999) Genetic analysis of susceptibility to dextran sulfate sodium-induced colitis in mice. Genomics 55:147–156CrossRefPubMed

Mallon PT, Mckenna M, Kirk SJ, Gardiner KR (2006) Dextran sulfate sodium (DSS) induced colitis reduces mucosal barrier function in Sprague-Dawley rats. Canadian Digestive Diseases Week (CDDW) February 24th–February 27th, 2006 ed. Banff, Alberta, Canadian Association of Gastroenterology

Marcus R, Watt J (1969) Seaweeds and ulcerative colitis in laboratory animals. Lancet 2:489–490CrossRefPubMed

Marcus R, Watt J (1971) Colonic ulceration in young rats fed degraded carrageenan. Lancet 2:765–766CrossRefPubMed

Murthy SN, Cooper HS, Shim H, Shah RS, Ibrahim SA, Sedergran DJ (1993) Treatment of dextran sulfate sodium-induced murine colitis by intracolonic cyclosporin. Dig Dis Sci 38:1722–1734CrossRefPubMed

Murthy S, Cooper HS, Yoshitake H, Meyer C, Meyer CJ, Murthy NS (1999) Combination therapy of pentoxifylline and tnfalpha monoclonal antibody in dextran sulphate-induced mouse colitis. Aliment Pharmacol Ther 13:251–260CrossRefPubMed

Ni J, Chen SF, Hollander D (1996) Effects of dextran sulphate sodium on intestinal epithelial cells and intestinal lymphocytes. Gut 39:234–241CrossRefPubMed

Ohkusa T (1985) production of experimental ulcerative colitis in hamsters by dextran sulfate sodium and changes in intestinal microflora. Nippon Shokakibyo Gakkai Zasshi 82:1327–1336PubMed

Okayasu I, Hatakeyama S, Yamada M, Ohkusa T, Inagaki Y, Nakaya R (1990) A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology 98:694–702PubMed

Poritz LS, Garver KI, Green C, Fitzpatrick L, Ruggiero F, Koltun WA (2007) Loss of the tight junction protein zo-1 in dextran sulfate sodium induced colitis. J Surg Res 140:12–19CrossRefPubMed

Stevceva L, Pavli P, Husband AJ, Doe WF (2001) The inflammatory infiltrate in the acute stage of the dextran sulphate sodium induced colitis: B cell response differs depending on the percentage of dss used to induce it. BMC Clin Pathol 1:3CrossRefPubMed

van Meeteren ME, Meijssen MA, Zijlstra FJ (2000) The effect of dexamethasone treatment on murine colitis. Scand J Gastroenterol 35:517–521CrossRefPubMed

Venkatraman A, Ramakrishna BS, Pulimood AB, Patra S, Murthy S (2000) Increased permeability in dextran sulphate colitis in rats: time course of development and effect of butyrate. Scand J Gastroenterol 35:1053–1059CrossRefPubMed

Verdu EF, Bercik P, Cukrowska B, Farre-Castany MA, Bouzourene H, Saraga E, Blum AL, Corthesy-Theulaz I, Tlaskalova-Hogenova H, Michetti P (2000) Oral administration of antigens from intestinal flora anaerobic bacteria reduces the severity of experimental acute colitis in balb/c mice. Clin Exp Immunol 120:46–50CrossRefPubMed

Watt J, Marcus R (1969) Ulcerative colitis in the guinea-pig caused by seaweed extract. J Pharm Pharmacol 21(Suppl):187S+

Yamada M, Ohkusa T, Okayasu I (1992) Occurrence of dysplasia and adenocarcinoma after experimental chronic ulcerative colitis in hamsters induced by dextran sulphate sodium. Gut 33:1521–1527CrossRefPubMed

Yoshida Y, Iwai A, Itoh K, Tanaka M, Kato S, Hokari R, Miyahara T, Koyama H, Miura S, Kobayashi M (2000) Role of inducible nitric oxide synthase in dextran sulphate sodium-induced colitis. Aliment Pharmacol Ther 14:26–32CrossRefPubMed

Title: The dextran sulphate sodium (DSS) model of colitis: an overview
Authors: Livingstone Solomon
Sorsiah Mansor
Peter Mallon
Eilish Donnelly
Margaret Hoper
Morris Loughrey
Stephen Kirk
Keith Gardiner
Publication date: 01-06-2010
Publisher: Springer-Verlag
Published in: Comparative Clinical Pathology / Issue 3/2010
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-010-0979-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The dextran sulphate sodium (DSS) model of colitis: an overview

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2010

Erratum to: Survey of Clostridium botulinum toxins in Iranian traditional food products

Haematological changes of cats with Toxoplasma gondii-specific antibodies

Effect of topical 0.5% betaxolol administration on intraocular pressure in clinically normal dogs

Nodular dermatitis as an atypical form of canine demodicosis in an English bulldog

Morphological observations of the thrombocyte of white-bellied sea eagle Haliaeetus leucogaster

Successful management of a case of downer syndrome in a crossbred cow after 45 days of recumbency