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01-06-2009 | Original Article

Small-Intestinal Manifestations of Dextran Sulfate Sodium Consumption in Rats and Assessment of the Effects of Lactobacillus fermentum BR11

Authors: Mark S. Geier, Cassie L. Smith, Ross N. Butler, Gordon S. Howarth

Published in: Digestive Diseases and Sciences | Issue 6/2009

Abstract

The dextran sulfate sodium (DSS) colitis model has been utilized to screen for novel therapeutics for ulcerative colitis. Evidence suggests the small intestine may also be affected by DSS. We characterized the effects of DSS on the small intestine and assessed the potential for Lactobacillus fermentum BR11 to modify or normalize DSS-induced changes. Rats were allocated to three groups, Water + Vehicle, DSS + Vehicle, and DSS + L. fermentum BR11. BR11 was administered twice daily for 14 days. DSS (2%) was provided from days 7 to 14. Small-intestinal tissue was analyzed for sucrase activity, histology, and crypt cell proliferation. Increased ileum crypt depth and cell proliferation was observed in DSS-treated rats compared to controls (P < 0.05). BR11 normalized these parameters. While DSS predominantly induces colonic damage, minor morphological alterations were also detected in the distal small intestine. L. fermentum BR11 normalized these features.

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Title: Small-Intestinal Manifestations of Dextran Sulfate Sodium Consumption in Rats and Assessment of the Effects of Lactobacillus fermentum BR11
Authors: Mark S. Geier
Cassie L. Smith
Ross N. Butler
Gordon S. Howarth
Publication date: 01-06-2009
Publisher: Springer US
Published in: Digestive Diseases and Sciences / Issue 6/2009
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-008-0495-4

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