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International Journal of Colorectal Disease

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01-03-2012 | Original Article

Autofluorescence imaging and the quantitative intensity of fluorescence for evaluating the dysplastic grade of colonic neoplasms

Authors: Kentaro Moriichi, Mikihiro Fujiya, Ryu Sato, Toshie Nata, Yoshiki Nomura, Nobuhiro Ueno, Chisato Ishikawa, Yuhei Inaba, Takahiro Ito, Kotaro Okamoto, Hiroki Tanabe, Yusuke Mizukami, Jiro Watari, Yusuke Saitoh, Yutaka Kohgo

Published in: International Journal of Colorectal Disease | Issue 3/2012

Abstract

Background and aims

Autofluorescence imaging (AFI) is a novel technology which can capture fluorescence emitted from intestinal tissues. While AFI is useful for detecting colorectal neoplasms, it is unclear whether AFI can facilitate the diagnosis by differentiating the extent of dysplasia of colorectal neoplasms. This study investigated the efficacy of AFI in discriminating high-grade from low-grade adenoma.

Materials and methods

Sixty-seven patients who underwent colonoscopy with AFI were enrolled in this study. The AFI images obtained from 158 lesions in these patients were visually classified into four categories, namely, green (G), green with magenta spots (GM), magenta with green spots (MG), and magenta (M), according to their color intensities, immediately after the examination. The AFI images of the lesions were quantified using an image-analytical software program (F index). Either the F index or the visual assessment was prospectively compared with the dysplastic grade.

Results

The F index of the high-grade adenomas was significantly lower than that of the low-grade adenomas, hyperplasia, and normal mucosa (p < 0.05). The incidence of the lesions classified into the M classification for high-grade adenomas (55.6%) was significantly higher than that of either low-grade adenomas (20.8%) or hyperplasia (0%). No correlation was observed between the F index or the visual classification and the tumor shape. The F index was not influenced by the size of the lesion, while the size was significantly associated with the visual classification of AFI.

Conclusions

AFI, particularly the F index, is considered to be a useful procedure for estimating the dysplastic grade of colonic adenomas.

Fearon ER, Vogelstein B (1990) A genetic model for colorectal tumorigenesis. Cell 61:759–767PubMedCrossRef

Lengauer C, Kinzler K, Vogelstein B (1998) Genetic instabilities in human cancers. Nature 396:643–649PubMedCrossRef

Baker SJ, Fearon ER, Nigro JM et al (1989) Chromosome 17 deletions and p53 gene mutations in colorectal carcinomas. Science 244:217–221PubMedCrossRef

Kamb A, Gruis NA, Weaver-Feldhaus J et al (1994) A cell cycle regulator potentially involved in genesis of many tumor types. Science 264:436–440PubMedCrossRef

Schlemper RJ, Riddell RH, Kato Y et al (2000) The Vienna classification of gastrointestinal epithelial neoplasia. Gut 47:251–255PubMedCrossRef

Takehana S, Kaneko M, Mizuno H (1999) Endoscopic diagnostic system using autofluorescence. Diagn Ther Endosc 5:59–63PubMedCrossRef

Nakashima A, Miwa H, Watanabe H et al (2001) A new technique: light-induced fluorescence endoscopy in combination with pharmacoendoscopy. Gastrointest Endosc 53:343–348

Georgakoudi I, Jacobson BC, van Dam J et al (2001) Fluorescence, reflectance, and light-scattering spectroscopy for evaluating dysplasia in patients with Barrett's esophagus. Gastroenterology 120:1620–1629PubMedCrossRef

Borovicka J, Fischer J, Neuweller J et al (2006) Autofluorescence endoscopy in surveillance of Barrett's esophagus. A multicenter randomized trial on diagnostic efficacy. Endoscopy 38:867–872PubMedCrossRef

10.

Kara MA, Bergman JJGH (2006) Autofluorescence imaging and narrow-band imaging for the detection of early neoplasia inpatients with Barrett's esophagus. Endoscopy 38:627–631PubMedCrossRef

11.

Uedo N, Iishi H, Tatsuta M et al (2005) A novel videoendoscopy system by using autofluorescence and reflectance imaging for diagnosis of esophagogastric cancers. Gastrointest Endosc 62:521–528PubMedCrossRef

12.

Matsuda T, Saito Y, Fu KI et al (2008) Does autofluorescence imaging videoendoscopy system improve the colonoscopic polyp detection rate?—a pilot study. Am J Gastroenterol 103:1926–1932PubMedCrossRef

13.

Messmann H, Endlicher E, Freunek G et al (2003) Fluorescence endoscopy for the detection of low and high grade dysplasia in ulcerative colitis using systemic or local 5-aminolaevulinic acid sensitization. Gut 52:1003–1007PubMedCrossRef

14.

Matsumoto T, Moriyama T, Yao T et al (2007) Autofluorescence imaging colonoscopy for the diagnosis of dysplasia in ulcerative colitis. Inflamm Bowel Dis 13:640–641PubMedCrossRef

15.

van den Broek FJ, Fockens P, van Eeden S et al (2008) Endoscopic tri-modal imaging for surveillance in ulcerative colitis: randomised comparison of high-resolution endoscopy and autofluorescence imaging for neoplasia detection; and evaluation of narrow-band imaging for classification of lesions. Gut 57:1083–1089PubMedCrossRef

16.

Fujiya M, Saitoh Y, Watari J et al (2007) Auto-fluorescence imaging is useful to assess the activity of ulcerative colitis. Dig Endosc 19:145–149CrossRef

17.

Ueno N, Fujiya M, Moriichi K, Ikuta K, Nata T, Konno Y, Ishikawa C, Inaba Y, Ito T, Sato R, Okamoto K, Tanabe H, Maemoto A, Sato K, Watari J, Ashida T, Saitoh Y, Kohgo Y (2011) Endosopic autofluorescence imaging is useful for the differential diagnosis of intestinal lymphomas resembling lymphoid heyperplasia. J Clin Gastroenterol 45:507–513PubMedCrossRef

18.

McCallum AL, Jenkins JT, Gillen D et al (2008) Evaluation of autofluorescence colonoscopy for the detection and diagnosis of colonic polyps. Gastrointest Endosc 68:283–290PubMedCrossRef

19.

Aihara H, Sumiyama K, Saito S, Tajiri H, Ikegami M (2009) Numerical analysis of the autofluorescence intensity of neoplastic and non-neoplastic colorectal lesions by using a novel videoendoscopy system. Gastrointest Endosc 69(3 Pt 2):726–733PubMedCrossRef

20.

Kudo S, Hirota S, Nakajima T et al (1994) Colorectal tumours and pit pattern. J Clin Pathol 47:880–885PubMedCrossRef

21.

Kudo S, Tamura S, Nakajima T et al (1996) Diagnosis of colorectal tumorous lesions by magnifying endoscopy. Gastrointest Endosc 44:8–14PubMedCrossRef

22.

Gono K, Yamazaki K, Doguchi N et al (2003) Endoscopic observation of tissue by narrow band illumination. Opt Rev 10:1–5CrossRef

23.

Gono K, Obi T, Yamaguchi M et al (2004) Appearance of enhanced tissue features in narrow-band endoscopic imaging. J Biomed Opt 9:568–577PubMedCrossRef

24.

Machida H, Sano Y, Hamamoto Y et al (2004) Narrow-band imaging in the diagnosis of colorectal mucosal lesions: a pilot study. Endoscopy 36:1094–1098PubMedCrossRef

25.

Hirata M, Tanaka S, Oka S et al (2007) Magnifying endoscopy with narrow band imaging for diagnosis of colorectal tumors. Gastrointest Endosc 65:988–995PubMedCrossRef

26.

Chiu HM, Chang CY, Chen CC et al (2007) A prospective comparative study of narrow-band imaging, chromoendoscopy, and conventional colonoscopy in the diagnosis of colorectal neoplasia. Gut 56:373–379PubMedCrossRef

27.

Tischendorf JJ, Wasmuth HE, Koch A et al (2007) Value of magnifying chromoendoscopy and narrow band imaging (NBI) in classifying colorectal polyps: a prospective controlled study. Endoscopy 39:1092–1096PubMedCrossRef

28.

Katagiri A, Fu KI, Sano Y et al (2008) Narrow band imaging with magnifying colonoscopy as diagnostic tool for predicting histology of early colorectal neoplasia. Aliment Pharmacol Ther 27:1269–1274PubMedCrossRef

Title: Autofluorescence imaging and the quantitative intensity of fluorescence for evaluating the dysplastic grade of colonic neoplasms
Authors: Kentaro Moriichi
Mikihiro Fujiya
Ryu Sato
Toshie Nata
Yoshiki Nomura
Nobuhiro Ueno
Chisato Ishikawa
Yuhei Inaba
Takahiro Ito
Kotaro Okamoto
Hiroki Tanabe
Yusuke Mizukami
Jiro Watari
Yusuke Saitoh
Yutaka Kohgo
Publication date: 01-03-2012
Publisher: Springer-Verlag
Published in: International Journal of Colorectal Disease / Issue 3/2012
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-011-1311-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Autofluorescence imaging and the quantitative intensity of fluorescence for evaluating the dysplastic grade of colonic neoplasms

Abstract

Background and aims

Materials and methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background and aims

Materials and methods

Results

Conclusions

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