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Arthritis Research & Therapy
Published in: Arthritis Research & Therapy 1/2020

01-12-2020 | Knee Osteoarthritis | Research article

Biological potential alterations of migratory chondrogenic progenitor cells during knee osteoarthritic progression

Authors: Yu-Xing Wang, Zhi-Dong Zhao, Qian Wang, Zhong-Li Li, Ya Huang, Sen Zhao, Wei Hu, Jia-Wu Liang, Pei-Lin Li, Hua Wang, Ning Mao, Chu-Tse Wu, Heng Zhu

Published in: Arthritis Research & Therapy | Issue 1/2020

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Abstract

Background

Although increasing studies have demonstrated that chondrogenic progenitor cells (CPCs) remain present in human osteoarthritic cartilage, the biological alterations of the CPCs from the less diseased lateral tibial condyle and the more diseased medial condyle of same patient remain to be investigated.

Methods

CPCs were isolated from paired grade 1–2 and grade 3–4 osteoarthritic cartilage by virtue of cell migratory capacities. The cell morphology, immunophenotype, self-renewal, multi-differentiation, and cell migration of these CPCs were evaluated. Additionally, the distributions of CD105+/CD271+ cells in OA osteochondral specimen were determined. Furthermore, a high-throughput mRNA sequencing was performed.

Results

Migratory CPCs (mCPCs) robustly outgrew from mildly collagenases-digested osteoarthritic cartilages. The mCPCs from grade 3–4 cartilages (mCPCs, grades 3–4) harbored morphological characteristics, cell proliferation, and colony formation capacity that were similar to those of the mCPCs from the grade 1–2 OA cartilages (mCPCs, grades 1–2). However, the mCPCs (grades 3–4) highly expressed CD271. In addition, the mCPCs (grades 3–4) showed enhanced osteo-adipogenic activities and decreased chondrogenic capacity. Furthermore, the mCPCs (grades 3–4) exhibited stronger cell migration in response to osteoarthritis synovial fluids. More CD105+/CD271+ cells resided in grade 3–4 articular cartilages. Moreover, the results of mRNA sequencing showed that mCPCs (grades 3–4) expressed higher migratory molecules.

Conclusions

Our data suggest that more mCPCs (grades 3–4) migrate to injured articular cartilages but with enhanced osteo-adipogenic and decreased chondrogenic capacity, which might explain the pathological changes of mCPCs during the progression of OA from early to late stages. Thus, these dysfunctional mCPCs might be optional cell targets for OA therapies.
Appendix
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Metadata
Title
Biological potential alterations of migratory chondrogenic progenitor cells during knee osteoarthritic progression
Authors
Yu-Xing Wang
Zhi-Dong Zhao
Qian Wang
Zhong-Li Li
Ya Huang
Sen Zhao
Wei Hu
Jia-Wu Liang
Pei-Lin Li
Hua Wang
Ning Mao
Chu-Tse Wu
Heng Zhu
Publication date
01-12-2020
Publisher
BioMed Central
Published in
Arthritis Research & Therapy / Issue 1/2020
Electronic ISSN: 1478-6362
DOI
https://doi.org/10.1186/s13075-020-2144-z

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