Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Archives of Virology
Published in: Archives of Virology 3/2024

01-03-2024 | Brief Report

Isolation and genome-wide analysis of the novel Acinetobacter baumannii bacteriophage vB_AbaM_AB3P2

Authors: Yujing Tan, Jianhui Su, Dandan Luo, Bingshao Liang, Shenshen Liu, Haiyan Zeng

Published in: Archives of Virology | Issue 3/2024

Login to get access

Abstract

A lytic Acinetobacter baumannii phage, isolate vB_AbaM_AB3P2, was isolated from a sewage treatment plant in China. A. baumannii phage vB_AbaM_AB3P2 has a dsDNA genome that is 44,824 bp in length with a G + C content of 37.75%. Ninety-six open reading frames were identified, and no genes for antibiotic resistance or virulence factors were found. Genomic and phylogenetic analysis of this phage revealed that it represents a new species in the genus Obolenskvirus. Phage vB_AbaM_AB3P2 has a short latent period (10 min) and high stability at 30–70°C and pH 2–10 and is potentially useful for controlling multi-drug-resistant A. baumannii.
Appendix
Available only for authorised users
Literature
1.
Dijkshoorn L, Nemec A, Seifert H (2007) An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii. Nat Rev Microbiol 5:939–951CrossRefPubMed
2.
Ibrahim S, Al-Saryi N, Al-Kadmy IMS, Aziz SN (2021) Multidrug-resistant Acinetobacter baumannii as an emerging concern in hospitals. Mol Biol Rep 48:6987–6998CrossRefPubMedPubMedCentral
3.
Chang RYK, Nang SC, Chan HK, Li J (2022) Novel antimicrobial agents for combating antibiotic-resistant bacteria. Adv Drug Deliv Rev 187:114378CrossRefPubMed
4.
Tan Y, Su J, Fu M, Zhang H, Zeng H (2023) Recent Advances in Phage-Based Therapeutics for Multi-Drug Resistant Acinetobacter baumannii. Bioeng (Basel Switzerland) 10(1):35
5.
Peng W, Zeng F, Wu Z, Jin Z, Li W, Zhu M, Wang Q, Tong Y, Chen L, Bai Q (2021) Isolation and genomic analysis of temperate phage 5W targeting multidrug-resistant Acinetobacter baumannii. Arch Microbiol 204:58CrossRefPubMed
6.
7.
Gill JJ, Hyman P (2010) Phage choice, isolation, and preparation for phage therapy. Curr Pharm Biotechnol 11:2–14CrossRefPubMed
8.
Zeng H, Li C, Luo D, Zhang J, Ding Y, Chen M, Yang X, Lei T, Wu S, Ye Q, Pang R, Gu Q, Wu Q (2021) Novel phage vB_CtuP_B1 for controlling Cronobacter malonaticus and Cronobacter turicensis in ready-to-eat lettuce and powered infant formula. Food research international (Ottawa, Ont) 143:110255
9.
Pehde BM, Niewohner D, Keomanivong FE, Carruthers MD (2021) Genome Sequence and Characterization of Acinetobacter Phage DMU1. PHAGE (New Rochelle, NY) 2:50–56
10.
Duan S, Dong Y, Xu Y, Yin J, Geng L, Xiu Z (2022) One-step salting-out extraction of bacteriophage from its infection broth of Acinetobacter baumannii. J Chromatogr A 1679:463407CrossRefPubMed
11.
Kropinski AM (2018) Practical Advice on the One-Step Growth Curve. Methods in molecular biology. (Clifton NJ) 1681:41–47
12.
Jończyk-Matysiak E, Łodej N, Kula D, Owczarek B, Orwat F, Międzybrodzki R, Neuberg J, Bagińska N, Weber-Dąbrowska B, Górski A (2019) Factors determining phage stability/activity: challenges in practical phage application. Expert Rev anti-infective therapy 17:583–606CrossRefPubMed
13.
Soleimani-Delfan A, Bouzari M, Wang R (2021) A rapid competitive method for bacteriophage genomic DNA extraction. J Virol Methods 293:114148CrossRefPubMed
14.
Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV, Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA (2012) SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput biology: J Comput Mol cell biology 19:455–477MathSciNetCrossRef
15.
Bouras G, Nepal R, Houtak G, Psaltis AJ, Wormald PJ, Vreugde S (2023) Pharokka: a fast scalable bacteriophage annotation tool. Bioinf (Oxford England) 39(1):btac776
16.
Jiang JZ, Yuan WG, Shang J, Shi YH, Yang LL, Liu M, Zhu P, Jin T, Sun Y, Yuan LH (2023) Virus classification for viral genomic fragments using PhaGCN2. Brief Bioinform 24(1):bbac505CrossRefPubMed
17.
Moraru C, Varsani A, Kropinski AM (2020) VIRIDIC-A Novel Tool to Calculate the Intergenomic Similarities of Prokaryote-Infecting Viruses. Viruses 12(11):1268CrossRefPubMedPubMedCentral
18.
Price MN, Dehal PS, Arkin AP (2009) FastTree: computing large minimum evolution trees with profiles instead of a distance matrix. Mol Biol Evol 26:1641–1650CrossRefPubMedPubMedCentral
19.
Sullivan MJ, Petty NK, Beatson SA (2011) Easyfig: a genome comparison visualizer. Bioinf (Oxford England) 27:1009–1010
20.
Sisakhtpour B, Mirzaei A, Karbasizadeh V, Hosseini N, Shabani M, Moghim S (2022) The characteristic and potential therapeutic effect of isolated multidrug-resistant Acinetobacter baumannii lytic phage. Ann Clin Microbiol Antimicrob 21:1CrossRefPubMedPubMedCentral
21.
Zhu Y, Han K, Chen L, Luo S, Fan H, Tong Y (2022) Biological characterization and genomic analysis of Acinetobacter baumannii phage BUCT628. Arch Virol 167:1471–1474CrossRefPubMed
22.
Zhang L, Wang X, Hua X, Yu Y, Leptihn S, Loh B (2022) Therapeutic evaluation of the Acinetobacter baumannii phage Phab24 for clinical use. Virus Res 320:198889CrossRefPubMed
23.
Mardiana M, Teh SH, Lin LC, Lin NT (2022) Isolation and Characterization of a Novel Siphoviridae Phage, vB_AbaS_TCUP2199, Infecting Multidrug-Resistant Acinetobacter baumannii. Viruses 14(6):1240CrossRefPubMedPubMedCentral
24.
Ma G, Gao X, Jiang C, Xing S, Wei C, Huang P, Lin J (2019) pH-Responsive Nanoprobe for In Vivo Photoacoustic Imaging of Gastric Acid. Anal Chem 91:13570–13575CrossRefPubMed
25.
26.
Lai MJ, Soo PC, Lin NT, Hu A, Chen YJ, Chen LK, Chang KC (2013) Identification and characterisation of the putative phage-related endolysins through full genome sequence analysis in Acinetobacter baumannii ATCC 17978. Int J Antimicrob Agents 42:141–148CrossRefPubMed
Metadata
Title
Isolation and genome-wide analysis of the novel Acinetobacter baumannii bacteriophage vB_AbaM_AB3P2
Authors
Yujing Tan
Jianhui Su
Dandan Luo
Bingshao Liang
Shenshen Liu
Haiyan Zeng
Publication date
01-03-2024
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 3/2024
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-024-05986-9

Other articles of this Issue 3/2024

Archives of Virology 3/2024 Go to the issue

Annotated Sequence Record

The complete genome sequences of a negative single-stranded RNA virus and a double-stranded RNA virus coinfecting the entomopathogenic fungus Beauveria bassiana Vuillemin

Brief Report

A single amino acid at position 31 in the N-terminus of the coat protein of cucumber mosaic virus determines its avirulence function for RCY1-conferred virus resistance

Original Article

Identification and characterization of a marine bacterium extract from Mameliella sp. M20D2D8 with antiviral effects against influenza A and B viruses

Original Article

Generation of porcine circovirus type 4 virus-like particles and their use to detect serum antibodies

Review

Current trends and new approaches for human norovirus replication in cell culture: a literature review

Review

The role of pyroptosis in viral infection
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits