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01-06-2021 | Ionizing Radiation | Original Article

Effect of ionizing radiation and cariogenic biofilm challenge on root-dentin caries

Authors: Camila de Carvalho Almança Lopes, Renata Borges Rodrigues, Maximiliano Sérgio Cenci, Juliana Lays Stolfo Uehara, Tamires Timm Maske, Pedro Henrique Justino Oliveira Limirio, Priscilla Barbosa Ferreira Soares, Veridiana Resende Novais

Published in: Clinical Oral Investigations | Issue 6/2021

Abstract

Objectives

To evaluate the effect of ionizing radiation and cariogenic biofilm challenge using two continuous flow models, normal and reduced salivary flow, on the development of initial root-dentin caries lesions.

Materials and methods

Microcosm biofilms were grown under two salivary flow rates (0.06 and 0.03 mL min⁻¹) and exposed to 5% sucrose (3 × daily, 0.25 mL min⁻¹, 6 min) dripped over non-irradiated and irradiated root-dentin blocks for up to 7 days. The vibration modes of root dentin, matrix/mineral (M/M), and carbonate/mineral (C/M) ratios were evaluated by FTIR. The mineral density was assessed by micro-CT.

Results

With normal salivary flow, FTIR revealed an increase in the organic matrix (amide III) and a decrease in the mineral phase (ν_4, ν₂ PO₄³⁻, AII + ν₂ CO₃²⁻, C/M) in caries lesions. Irradiated dentin exhibited a reduction in the mineral phase (ν₁, ν₃ PO₄³⁻, ν₂ CO₃²⁻, C/M). Differences in mineral densities were not significant. With reduced salivary flow, FTIR also revealed increased organic matrix (amide III) for irradiated caries lesions and decrease in mineral phase (v_4, v₂ PO₄³⁻, v₂ CO₃²⁻, and C/M) in caries lesions. ν₁, ν₃ PO₄³⁻ precipitated on the surface of irradiated dentin and a lower mineral density was observed.

Conclusions

Initial caries lesions differed between non-irradiated and irradiated dentin and between normal and reduced salivary flow rates. Significant mineral loss with exposure of the organic matrix and low mineral density were observed for irradiated dentin with a reduced salivary flow rate.

Clinical relevance

Ionizing radiation associated with a reduced salivary flow rate enhanced the progression of root-dentin caries.

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Title: Effect of ionizing radiation and cariogenic biofilm challenge on root-dentin caries
Authors: Camila de Carvalho Almança Lopes
Renata Borges Rodrigues
Maximiliano Sérgio Cenci
Juliana Lays Stolfo Uehara
Tamires Timm Maske
Pedro Henrique Justino Oliveira Limirio
Priscilla Barbosa Ferreira Soares
Veridiana Resende Novais
Publication date: 01-06-2021
Publisher: Springer Berlin Heidelberg
Keywords: Ionizing Radiation
Caries
Published in: Clinical Oral Investigations / Issue 6/2021
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-020-03736-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effect of ionizing radiation and cariogenic biofilm challenge on root-dentin caries

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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