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01-11-2018 | Original Article

Radiotherapy alters the composition, structural and mechanical properties of root dentin in vitro

Authors: Marilia Mattar de Amoêdo Campos Velo, Ana Laura Herrera Farha, Paulo Sérgio da Silva Santos, Aymée Shiota, Simone Zuquerato Sansavino, Ana Tarsila Souza, Heitor Marques Honório, Linda Wang

Published in: Clinical Oral Investigations | Issue 8/2018

Abstract

Objectives

Post-radiation dental lesions affect mainly the cervical area of the tooth. Until now, there are quite few evidences regarding the effects of radiation exposure on root dentin breakdown. To better understand this effect, we used human root dentin specimens obtained from third molars from similarly aged individuals.

Materials and methods

Twenty specimens were analyzed by the surface hardness (SH), energy-dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD) to evaluate the baseline properties of their root dentin. Other six human teeth were prepared and analyzed by scanning electron microscopy (SEM). Then the specimens were randomly distributed between two groups (n = 13 per group) and irradiated with a total dose of 55 or 70 Gy in a linear accelerator. The percentage of EDX and surface hardness loss (%SHL) were determined based on measurements before and after irradiation. The specimens were also analyzed after irradiation by SEM and XRD. The Ca/P weight ratio was calculated.

Results

Based on SEM analysis, radiation exposure induced dehydration of the dentin. The Ca/P weight ratio decreased (p = 0.0045). The %SHL of specimens irradiated with 70 Gy was higher than that of the 55-Gy group (p < 0.05), although even the lower dose induced root dentin breakdown.

Conclusions

Overall, we can state that radiation exposure changes the composition and structure of human root dentin, which detrimentally affect its hardness.

Clinical relevance

The changes reported herein might influence the selection of the dental materials and will bring new knowledge in this field to prevent radiation-related caries in root dentin.

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Title: Radiotherapy alters the composition, structural and mechanical properties of root dentin in vitro
Authors: Marilia Mattar de Amoêdo Campos Velo
Ana Laura Herrera Farha
Paulo Sérgio da Silva Santos
Aymée Shiota
Simone Zuquerato Sansavino
Ana Tarsila Souza
Heitor Marques Honório
Linda Wang
Publication date: 01-11-2018
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 8/2018
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2373-6

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Radiotherapy alters the composition, structural and mechanical properties of root dentin in vitro

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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