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Journal of Cardiovascular Translational Research

Published in:

01-02-2022 | Original Article

Intracellular Ca²⁺ Dysregulation in Coronary Smooth Muscle Is Similar in Coronary Disease of Humans and Ossabaw Miniature Swine

Authors: Jill K. Badin, Caleb Eggenberger, Stacey Dineen Rodenbeck, Zubair A. Hashmi, I-wen Wang, Jose P. Garcia, Mouhamad Alloosh, Michael Sturek

Published in: Journal of Cardiovascular Translational Research | Issue 1/2022

Abstract

Intracellular free Ca²⁺ ([Ca²⁺]_i) dysregulation occurs in coronary smooth muscle (CSM) in atherosclerotic coronary artery disease (CAD) of metabolic syndrome (MetS) swine. Our goal was to determine how CAD severity, arterial structure, and MetS risk factors associate with [Ca²⁺]_i dysregulation in human CAD compared to changes in Ossabaw miniature swine. CSM cells were dispersed from coronary arteries of explanted hearts from transplant recipients and from lean and MetS swine with CAD. CSM [Ca²⁺]_i elicited by Ca²⁺ influx and sarcoplasmic reticulum (SR) Ca²⁺ release and sequestration was measured with fura-2. Increased [Ca²⁺]_i signaling was associated with advanced age and a greater media area in human CAD. Decreased [Ca²⁺]_i signaling was associated with a greater number of risk factors and a higher plaque burden in human and swine CAD. Similar [Ca²⁺]_i dysregulation exhibited in human and Ossabaw swine CSM provides strong evidence for the translational relevance of this large animal model.

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Title: Intracellular Ca2+ Dysregulation in Coronary Smooth Muscle Is Similar in Coronary Disease of Humans and Ossabaw Miniature Swine
Authors: Jill K. Badin
Caleb Eggenberger
Stacey Dineen Rodenbeck
Zubair A. Hashmi
I-wen Wang
Jose P. Garcia
Mouhamad Alloosh
Michael Sturek
Publication date: 01-02-2022
Publisher: Springer US
Published in: Journal of Cardiovascular Translational Research / Issue 1/2022
Print ISSN: 1937-5387
Electronic ISSN: 1937-5395
DOI: https://doi.org/10.1007/s12265-021-10153-5

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Intracellular Ca²⁺ Dysregulation in Coronary Smooth Muscle Is Similar in Coronary Disease of Humans and Ossabaw Miniature Swine

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