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Open Access 01-11-2021 | Intoxication | Short Communication

Quantitative evidence of suppressed TMEM119 microglial immunohistochemistry in fatal morphine intoxications

Authors: Simone Bohnert, Kosmas Georgiades, Camelia-Maria Monoranu, Michael Bohnert, Andreas Büttner, Benjamin Ondruschka

Published in: International Journal of Legal Medicine | Issue 6/2021

Abstract

The aim of this pilot study was to investigate the diagnostic potential of TMEM119 as a useful microglia-specific marker in combination with immunostainings for phagocytic function and infiltrating capacity of monocytes in cases of lethal monosubstance intoxications by morphine (MOR), methamphetamine (METH), and of ethanol-associated death (ETH) respectively. Human brain tissue samples were obtained from forensic autopsies of cases with single substance abuse (MOR, n = 8; ETH, n = 10; METH, n = 9) and then compared to a cohort of cardiovascular fatalities as controls (n = 9). Brain tissue samples of cortex, white matter, and hippocampus were collected and stained immunohistochemically with antibodies against TMEM119, CD68KiM1P, and CCR2. We could document the lowest density of TMEM119-positive cells in MOR deaths with highly significant differences to the control densities in all three regions investigated. In ETH and METH deaths, the expression of TMEM119 was comparable to cell densities in controls. The results indicate that the immunoreaction in brain tissue is different in these groups depending on the drug type used for abuse.

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Title: Quantitative evidence of suppressed TMEM119 microglial immunohistochemistry in fatal morphine intoxications
Authors: Simone Bohnert
Kosmas Georgiades
Camelia-Maria Monoranu
Michael Bohnert
Andreas Büttner
Benjamin Ondruschka
Publication date: 01-11-2021
Publisher: Springer Berlin Heidelberg
Keywords: Intoxication
Morphine
Published in: International Journal of Legal Medicine / Issue 6/2021
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-021-02699-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Quantitative evidence of suppressed TMEM119 microglial immunohistochemistry in fatal morphine intoxications

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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