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Respiratory Research
Published in: Respiratory Research 1/2019

Open Access 01-12-2019 | Influenza Virus | Research

Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma

Authors: Rebecca L. Vanders, Alan Hsu, Peter G. Gibson, Vanessa E. Murphy, Peter A. B. Wark

Published in: Respiratory Research | Issue 1/2019

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Abstract

Respiratory virus-induced asthma exacerbations occur frequently during pregnancy and are associated with adverse outcomes for mother and child. Primary nasal epithelial cells (pNECs) provide a useful method to study immune responses in pregnancy. pNECs were obtained by nasal brushings from pregnant and non-pregnant women with and without asthma. pNECS were infected in vitro with major group Rhinovirus 43 (RV43) and seasonal influenza (H3N2). Following infection, pNECs showed measurable quantities of interferon (IFN)-λ, IL-1β, IL-8, IP-10 and MIP1-α. pNECs provide a safe and effective method for studying respiratory epithelial cell responses during pregnancy.
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Metadata
Title
Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma
Authors
Rebecca L. Vanders
Alan Hsu
Peter G. Gibson
Vanessa E. Murphy
Peter A. B. Wark
Publication date
01-12-2019
Publisher
BioMed Central
Published in
Respiratory Research / Issue 1/2019
Electronic ISSN: 1465-993X
DOI
https://doi.org/10.1186/s12931-019-1225-5

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