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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-04-2021 | Indomethacin | Basic Science

Subconjunctival injection of tumor necrosis factor-α pre-stimulated bone marrow-derived mesenchymal stem cells enhances anti-inflammation and anti-fibrosis in ocular alkali burns

Authors: Nuan Zhang, Xiaohui Luo, Shiyao Zhang, Ren Liu, Lingyi Liang, Wenru Su, Dan Liang

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 4/2021

Abstract

Purpose

To investigate the therapeutic effect of subconjunctival injection of tumor necrosis factor-α (TNF-α) pre-stimulated bone marrow-derived mesenchymal stem cells (BMMSCs) on ocular alkali burns in a rat model.

Methods

After applying a 6 mm filter paper soaking in 1 N NaOH on the cornea of rats, the suspension of TNF‐α pre-stimulated BMMSCs, BMMSCs and PBS were given subconjunctivally and respectively. Corneal epithelial defect, corneal opacity, inflammation as well as PTGS2 and TSG-6 expression on day 7 and fibrosis on day 14 were compared.

Results

TNF‐α pre-stimulated BMMSCs group had a more predominate effect on promoting corneal epithelial repairing, decreasing corneal opacity, reducing inflammatory cells and CD68 + macrophages on day 7 and suppressing fibrosis on day 14 compared to BMMSCs group. Besides, it had significant increased expressions of PTGS2 and TSG-6 in vitro. Pre-treated with Indomethacin revealed a reverse effect on above-mentioned changes.

Conclusion

Subconjunctival injection of TNF‐α pre-stimulated BMMSCs enhanced anti-inflammatory and anti-fibrotic effect in ocular alkali burns, which was possibly though up regulation of PTGS2 and TSG-6 expression.

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Title: Subconjunctival injection of tumor necrosis factor-α pre-stimulated bone marrow-derived mesenchymal stem cells enhances anti-inflammation and anti-fibrosis in ocular alkali burns
Authors: Nuan Zhang
Xiaohui Luo
Shiyao Zhang
Ren Liu
Lingyi Liang
Wenru Su
Dan Liang
Publication date: 01-04-2021
Publisher: Springer Berlin Heidelberg
Keyword: Indomethacin
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 4/2021
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-020-05017-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Subconjunctival injection of tumor necrosis factor-α pre-stimulated bone marrow-derived mesenchymal stem cells enhances anti-inflammation and anti-fibrosis in ocular alkali burns

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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