Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ASCO Annual Meeting 2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

2024 ASCO Annual Meeting

Keep up to date with all the top stories and latest evidence with our hub page, including official congress videos and expert takeaways.
ADVANCED SEARCH
Log in
Top
BMC Oral Health
Published in: BMC Oral Health 1/2016

Open Access 01-12-2016 | Research article

Immunohistochemical expression of glucose transporter 1 in keratin-producing odontogenic cysts

Authors: Beatriz Vera-Sirera, Leopoldo Forner-Navarro, Francisco Vera-Sempere

Published in: BMC Oral Health | Issue 1/2016

Login to get access

Abstract

Background

Keratin-producing odontogenic cysts (KPOCs) are a group of cystic lesions that are often aggressive, with high rates of recurrence and multifocality. KPOCs included orthokeratinised odontogenic cyst (OOC) and parakeratotic odontogenic cysts, which are now considered true tumours denominated keratocystic odontogenic tumours (KCOTs). GLUT1 is a protein transporter that is involved in the active uptake of glucose across cell membranes and that is overexpressed in tumours in close correlation with the proliferation rate and positron emission tomography (PET) imaging results.

Methods

A series of 58 keratin-producing odontogenic cysts was evaluated histologically and immunohistochemically in terms of GLUT1 expression. Different data were correlated using the beta regression model in relation to histological type and immunohistochemical expression of GLUT1, which was quantified using two different morphological methods.

Results

KPOC cases comprised 12 OOCs and 46 KCOTs, the latter corresponding to 6 syndromic and 40 sporadic KCOTs. GLUT1 expression was very low in OOC cases compared with KCOT cases, with statistical significant differences when quantification was considered. Different GLUT1 localisation patterns were revealed by immunostaining, with the parabasal cells showing higher reactivity in KCOTs. However, among KCOTs cases, GLUT1 expression was unable to establish differences between syndromic and sporadic cases.

Conclusions

GLUT1 expression differentiated between OOC and KCOT cases, with significantly higher expression in KCOTs, but did not differentiate between syndromic and sporadic KCOT cases. However, given the structural characteristics of KCOTs, we hypothesised that PET imaging methodology is probably not a useful diagnostic tool for KCOTs. Further studies of GLUT1 expression and PET examination in KCOT series are needed to confirm this last hypothesis.
Literature
1.
Shear M. The aggressive nature of the odontogenic keratocyst: it is a benign cystic neoplasm? part 1. Clinical and early experimental evidence of aggressive behaviour. Oral Oncol. 2002;38:219–26.CrossRefPubMed
2.
Gani F, Mahomed F, Meer S. Evaluation of Ki-67 and cyclin D1 expression in odontogenic keratocysts and orthokeratinised jaw cysts. SADJ. 2012;67:368–71.
3.
Vera-Sirera B, Forner-Navarro L, Vera-Sempere F. Differential expressión of cyclin D1 in keratin-producing odontogenic cysts. Med Oral Cir Bucal. 2014;20:e59–65.
4.
Barnes L, Eveson JW, Reichart P, Sidransky D. World Health Organization classification of tumours, Pathology and Genetics of Head and Neck tumours. IARC: Lyon; 2005.
5.
Johnson NR, Batstone MD, Savage NW. Management and recurrence of keratocystic odontogenic tumor: a systematic review. Oral Surg Oral Med Oral Pathol Oral Radiol. 2013;116:e271–6.CrossRefPubMed
6.
Barghava D, Deshpande A, Pogrel MA. Keratocystic odontogenic tumor (KCOT) - a cyst to a tumour. Oral Maxillofac Surg. 2012;16:163–70.CrossRef
7.
Wood IS, Trayhum P. Glucose transporters (GLUT and SGLT): expanded families of sugar transport proteins. Br J Nutr. 2002;89:3–9.CrossRef
8.
Smith TA. Facilitative glucose transporter expression in human cancer tissue. Br J Biomed Sci. 1999;56:285–92.PubMed
9.
Jones T, Price P. Development and experimental medicine applications of PET in oncology: a historical perspective. Lancet Oncol. 2012;13:e116–25.CrossRefPubMed
10.
Horiuchi C, Tsukuda M, Yaguchi T, Ishiguro Y, Okudera K, Inoue T. Correlation between FDG-PET findings and GLUT1 expression in salivary gland pleomorphic adenomas. Ann Nucl Med. 2008;22:693–8.CrossRefPubMed
11.
Shen WQ, Cheng KJ, Bao YY, Zhou SH, Yao HT. Expression of Glut-1; HIF-1α, PI3K and p-Akt in a case of ceruminous adenoma. Head Neck Oncol. 2012;4:18–21.CrossRefPubMedPubMedCentral
12.
Aiki M, Yoshimura H, Ohba S, Kimura S, Imamura Y, Sano K. Rapid growing myofibroma of the gingiva: report of a case and review of the literature. J Oral Maxillofac Surg. 2014;72:99–105.CrossRefPubMed
13.
Otsuru M, Auki T, Tsukinoki K, Ota Y, Karakida K, Yamazaki H, Yasuda M, Kaneko A. Usefulness of 18F-fluorodeoxyglucose positron emission tomography for detecting ameloblastoma, with special reference to glucosa transporter-1 expression. J Oral Maxillofac Surg. 2008;66:1761–5.CrossRefPubMed
14.
Nayak MT, Singh A, Sinhvi A, Sharma R. Odontogenic keratocyst: What is the name? J Natl Sci Biol Med. 2013;4:282–5.CrossRef
15.
Wright JM. The odontogenic keratocyst: orthokeratinized variant. Oral Surg Oral Med Oral Pathol. 1981;51:609–18.CrossRefPubMed
16.
Dong Q, Pan S, Sun LS, Li TJ. Orthokeratinized odontogenic cyst. A clinicopathological study of 61 cases. Arch Pathol Lab Med. 2010;134:271–5.PubMed
17.
Kimonis VE, Goldstein AM, Pastakia B. Clinical manifestations in 105 persons with nevoid basal cell carcinoma syndrome. Am J Med Genet. 1997;69:299–308.CrossRefPubMed
18.
Remmele W, Stegner HE. Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER-ICA) in breast cancer tissue. Pathologue. 1987;8:138–40.
19.
Oriuchi N, Higuchi T, Ishikita T, Miyakubo M, Hanaoka H, Iida Y, Endo K. Present role and future prospects of positron emission tomography in clinical oncology. Cancer Sci. 2006;97:1291–7.CrossRefPubMed
20.
Carvalho KC, Cunha IW, Rocha RM, Ayala FR, Cajaiba MM, Begnami MD, Vilela RS, Paiva GR, Andrade RG, Soares FA. GLUT1 expression in malignant tumors and its use as immunodiagnostic marker. Clinics (Sao Paulo). 2011;66:965–72.CrossRef
21.
Yonekura Y, Benua RS, Brill AB, et al. Increased accumulation of 2-deoxy-2-[18F]fluoro-D-glucose in liver metastasis from colon carcinoma. J Nucl Med. 1982;23:1133.PubMed
22.
Younes M, Ertan A, Lechago LV, Somoano J, Lechago J. Human erythrocyte glucose transporter (Glut1) is immunohistochemically detected as a late event during malignant progression in Barrett's metaplasia. Cancer Epidemiol Biomarkers Prev. 1997;6:303–5.PubMed
23.
24.
Highasi T, Saga T, Nakamoto Y, Ishimori T, Mamede MH, Wada M, Doi R, Hosotani R, Imamura M, Konishi J. Relationship between retention index in dual-phase (18)F-FDG PET, and hexokinase-II and glucose transporter-1 expression in pancreatic cancer. J Nucl Med. 2002;43:173–80.
25.
Deron P, Vangestel C, Goethals I, De Potter A, Peeters M, Vermeersch H, Van de Wiele C. FDG uptake in primary squamous cell carcinoma of the head and neck. The relationship between overexpression of glucose transporters and hexokinases, tumour proliferation and apoptosis. Nuklearmedizin. 2011;50:15–21.CrossRefPubMed
26.
Purohit BS, Ailianou A, Dulguerov N, Becker CD, Ratib O, Becker M. FDG-PET/CT pitfalls in oncological head and neck imaging. Insights Imaging. 2014;5:585–602.CrossRefPubMedPubMedCentral
27.
Hong R, Lim S-C. 18F-fluoro-2-deoxyglucose uptake on PET CT and glucose transporter 1 expression in colorectal adenocarcinoma. World J Gastroenterol. 2012;18:168–74.CrossRefPubMedPubMedCentral
Metadata
Title
Immunohistochemical expression of glucose transporter 1 in keratin-producing odontogenic cysts
Authors
Beatriz Vera-Sirera
Leopoldo Forner-Navarro
Francisco Vera-Sempere
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2016
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-016-0191-2

Other articles of this Issue 1/2016

BMC Oral Health 1/2016 Go to the issue

Research article

Salivary and serum inflammatory mediators among pre-conception women with periodontal disease

Research article

A randomised clinical trial to evaluate the effect of a 67 % sodium bicarbonate-containing dentifrice on 0.2 % chlorhexidine digluconate mouthwash tooth staining

Research article

Effect of a school-based oral health education in preventing untreated dental caries and increasing knowledge, attitude, and practices among adolescents in Bangladesh

Research article

PDGFRβ+/c-kit+ pulp cells are odontoblastic progenitors capable of producing dentin-like structure in vitro and in vivo

Research article

Factors associated with staying or leaving a dentist leader’s position – a qualitative study

Research article

RETRACTED ARTICLE: Human cathelicidin LL-37 enhance the antibiofilm effect of EGCG on Streptococcus mutans