Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Molecular Imaging and Biology
Published in: Molecular Imaging and Biology 2/2019

01-04-2019 | Brief Article

Imaging Pulmonary Foreign Body Reaction Using [125I]iodo-DPA-713 SPECT/CT in Mice

Authors: Julian Sanchez-Bautista, Catherine A. Foss, Alvaro A. Ordonez, Mariah H. Klunk, Sanjay K. Jain

Published in: Molecular Imaging and Biology | Issue 2/2019

Login to get access

Abstract

Purpose

Foreign body reactions elicit granulomatous inflammation composed of reactive macrophages. We hypothesized that [125I]iodo-DPA-713 single-photon emission computed tomography (SPECT), a low-molecular-weight pyrazolopyrimidine ligand selectively trapped by phagocytes, could be used to detect foreign body reactions in a murine model.

Procedures

C57BL/6 mice intratracheally inoculated with dextran beads, which developed foreign body lesions, were imaged after injection of [125I]iodo-DPA-713 or DPA-713-IRDye800CW using SPECT and optical imaging, respectively.

Results

Foreign body lesions were clearly observed in the lungs of the dextran-treated mice on computer tomography imaging and demonstrated significantly higher [125I]iodo-DPA-713 uptake compared with control animals (p < 0.01). Ex vivo studies demonstrated granulomatous reactions in the lungs of dextran-treated mice and localization of DPA-713-IRDye800CW at the diseased sites confirming the imaging findings.

Conclusion

Radioiodinated DPA-713 may be used as a noninvasive biomarker for the detection of pulmonary foreign body reactions.
Appendix
Available only for authorised users
Literature
1.
Rupprecht R, Papadopoulos V, Rammes G, Baghai TC, Fan J, Akula N, Groyer G, Adams D, Schumacher M (2010) Translocator protein (18 kDa) (TSPO) as a therapeutic target for neurological and psychiatric disorders. Nat Rev Drug Discov 9:971–988CrossRefPubMed
2.
3.
Foss CA, Harper JS, Wang H, Pomper MG, Jain SK (2013) Noninvasive molecular imaging of tuberculosis-associated inflammation with radioiodinated DPA-713. J Infect Dis 208:2067–2074CrossRefPubMedPubMedCentral
4.
Ordonez AA, Pokkali S, DeMarco VP et al (2015) Radioiodinated DPA-713 imaging correlates with bactericidal activity of tuberculosis treatments in mice. Antimicrob Agents Chemother 59:642–649CrossRefPubMed
5.
Anderson JM, Rodriguez A, Chang DT (2008) Foreign body reaction to biomaterials. Semin Immunol 20:86–100CrossRefPubMed
6.
Wang H, Pullambhatla M, Guilarte TR, Mease RC, Pomper MG (2009) Synthesis of [125I]iodoDPA-713: a new probe for imaging inflammation. Biochem Biophys Res Commun 389:80–83CrossRefPubMedPubMedCentral
7.
Foss CA, Liu L, Mease RC, Wang H, Pasricha P, Pomper MG (2017) Imaging macrophage accumulation in a murine model of chronic pancreatitis with 125I-iodo-DPA-713 SPECT/CT. J Nucl Med 58:1685–1690CrossRefPubMedPubMedCentral
8.
Foss CA, Bedja D, Mease RC, Wang H, Kass DA, Chatterjee S, Pomper MG (2015) Molecular imaging of inflammation in the ApoE -/- mouse model of atherosclerosis with iodoDPA. Biochem Biophys Res Commun 461:70–75CrossRefPubMedPubMedCentral
9.
Tucker EW, Pokkali S, Zhang Z, DeMarco VP, Klunk M, Smith ES, Ordonez AA, Penet MF, Bhujwalla Z, Jain SK, Kannan S (2016) Microglia activation in a pediatric rabbit model of tuberculous meningitis. Dis Model Mech 9:1497–1506CrossRefPubMedPubMedCentral
10.
Bendeck SE, Leung AN, Berry GJ, Daniel D, Ruoss SJ (2001) Cellulose granulomatosis presenting as centrilobular nodules: CT and histologic findings. AJR Am J Roentgenol 177:1151–1153CrossRefPubMed
11.
Radow SK, Nachamkin I, Morrow C, Fairman RP, Fratkin M, Rodriguez GE, Glauser FL (1983) Foreign body granulomatosis. Clinical and immunologic findings. Am Rev Respir Dis 127:575–580CrossRefPubMed
12.
James ML, Fulton RR, Henderson DJ, Eberl S, Meikle SR, Thomson S, Allan RD, Dolle F, Fulham MJ, Kassiou M (2005) Synthesis and in vivo evaluation of a novel peripheral benzodiazepine receptor PET radioligand. Bioorg Med Chem 13:6188–6194CrossRefPubMed
13.
Foss CA, Plyku D, Ordonez AA, Sanchez-Bautista J, Rosenthal HB, Minn IL, Lodge MA, Pomper MG, Sgouros G, Jain SK (2018) Biodistribution and radiation dosimetry of (124)I-iodo-DPA-713, a PET radiotracer for macrophage-associated inflammation. J Nucl Med:jnumed.117.207431
Metadata
Title
Imaging Pulmonary Foreign Body Reaction Using [125I]iodo-DPA-713 SPECT/CT in Mice
Authors
Julian Sanchez-Bautista
Catherine A. Foss
Alvaro A. Ordonez
Mariah H. Klunk
Sanjay K. Jain
Publication date
01-04-2019
Publisher
Springer International Publishing
Published in
Molecular Imaging and Biology / Issue 2/2019
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI
https://doi.org/10.1007/s11307-018-1249-0

Other articles of this Issue 2/2019

Molecular Imaging and Biology 2/2019 Go to the issue

Research Article

How Long of a Dynamic 3′-Deoxy-3′-[18F]fluorothymidine ([18F]FLT) PET Acquisition Is Needed for Robust Kinetic Analysis in Breast Cancer?

Research Article

SNAPshots of the MCHR1: a Comparison Between the PET-Tracers [18F]FE@SNAP and [11C]SNAP-7941

Review Article

Fluorescence Guidance in Surgical Oncology: Challenges, Opportunities, and Translation

Research Article

In Vivo Mapping and Quantification of Creatine Using Chemical Exchange Saturation Transfer Imaging in Rat Models of Epileptic Seizure

Research Article

Evaluation of Glucose Uptake and Uncoupling Protein 1 Activity in Adipose Tissue of Diabetic Mice upon β-Adrenergic Stimulation

Research Article

Quantitative Intensity Harmonization of Dopamine Transporter SPECT Images Using Gamma Mixture Models