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Molecular Imaging and Biology
Published in: Molecular Imaging and Biology 2/2019

01-04-2019 | Research Article

In Vivo Mapping and Quantification of Creatine Using Chemical Exchange Saturation Transfer Imaging in Rat Models of Epileptic Seizure

Authors: Dong-Hoon Lee, Do-Wan Lee, Jae-Im Kwon, Chul-Woong Woo, Sang-Tae Kim, Jin Seong Lee, Choong Gon Choi, Kyung Won Kim, Jeong Kon Kim, Dong-Cheol Woo

Published in: Molecular Imaging and Biology | Issue 2/2019

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Abstract

Purpose

To evaluate signal changes in the hippocampus of epileptic seizure rat models, based on quantified creatine chemical exchange saturation transfer (CrCEST) signals.

Procedures

CEST data and 1H magnetic resonance spectroscopy (1H MRS) data were obtained for the two imaging groups: control (CTRL) and epileptic seizure-induced (ES; via kainic acid [KA] injection) groups. CrCEST signals in the hippocampal regions were quantitatively evaluated; correlations between CrCEST signals and phosphocreatine (PCr) and total creatine (tCr; PCr + Cr) concentrations, derived from the analysis of 1H MRS data, were investigated as a function of time changes (before KA injection, 3 and 5 h after KA injection).

Results

Measured CrCEST signals were exhibited significant differences between before and after KA injection in the ES group. At each time point, CrCEST signals showed significant correlations with PCr concentration (all |r| > 0.59; all P < 0.05); no significant correlations were found between CrCEST signals and tCr concentrations (all |r| < 0.22; all P > 0.05).

Conclusions

CrCEST can adequately detect changes in the concentration of Cr as a result of energy metabolism, and may serve as a potentially useful tool for diagnosis and assessment of prognosis in epilepsy.
Literature
1.
2.
Mikati MA, Kurdit RM, Rahmeh AA et al (2004) Effects of creatine and cyclocreatine supplementation on kainate induced injury in pre-pubescent rats. Brain Inj 18:1229–1241CrossRefPubMed
3.
Tokumitsu T, Mancuso A, Weinstein PR, Weiner MW, Naruse S, Maudsley AA (1997) Metabolic and pathological effects of temporal lobe epilepsy in rat brain detected by proton spectroscopy and imaging. Brain Res 744:57–67CrossRefPubMedPubMedCentral
4.
Tai XY, Bernhardt B, Thom M, Thompson P, Baxendale S, Koepp M, Bernasconi N (2018) Review: neurodegenerative processes in temporal lobe epilepsy with hippocampal sclerosis: clinical, pathological and neuroimaging evidence. Neuropathol Appl Neurobiol 44:70–90CrossRefPubMed
5.
6.
Deleo F, Thom M, Concha L et al (2017) Histological and MRI markers of white matter damage in focal epilepsy. Epilepsy Res 140:29–38CrossRefPubMed
7.
Ruber T, David B, Elger CE (2018) MRI in epilepsy: clinical standard and evolution. Curr Opin Neurol 31:223–231CrossRefPubMed
8.
Wong-Kisiel LC, Tovar Quiroga DF, Kenney-Jung DL, Witte RJ, Santana-Almansa A, Worrell GA, Britton J, Brinkmann BH (2018) Morphometric analysis on T1-weighted MRI complements visual MRI review in focal cortical dysplasia. Epilepsy Res 140:184–191CrossRefPubMed
9.
Fei P, Soucy JP, Obaid S et al (2018) The value of regional cerebral blood flow SPECT and FDG PET in Operculo-insular epilepsy. Clin Nucl Med 43:e67–e73CrossRefPubMed
10.
Jafari-Khouzani K, Elisevich K, Karvelis KC, Soltanian-Zadeh H (2011) Quantitative multi-compartmental SPECT image analysis for lateralization of temporal lobe epilepsy. Epilepsy Res 95:35–50CrossRefPubMedPubMedCentral
11.
Long Z, Hanson DP, Mullan BP, Hunt CH, Holmes DR III, Brinkmann BH, O'Connor MK (2018) Analysis of brain SPECT images coregistered with MRI in patients with epilepsy: comparison of three methods. J Neuroimaging 28:307–312CrossRefPubMed
12.
Shiga T, Suzuki A, Sakurai K, Kurita T, Takeuchi W, Toyonaga T, Hirata K, Kobashi K, Katoh C, Kubo N, Tamaki N (2017) Dual isotope SPECT study with epilepsy patients using semiconductor SPECT system. Clin Nucl Med 42:663–668CrossRefPubMed
13.
Calabria FF, Cascini GL, Gambardella A et al (2017) Ictal 18F-FDG PET/MRI in a patient with cortical heterotopia and focal epilepsy. Clin Nucl Med 42:768–769CrossRefPubMed
14.
Nardetto L, Zoccarato M, Santelli L, Tiberio I, Cecchin D, Giometto B (2017) 18F-FDG PET/MRI in cryptogenic new-onset refractory status epilepticus: a potential marker of disease location, activity and prognosis? J Neurol Sci 381:100–102CrossRefPubMed
15.
House PM, Lanz M, Holst B, Martens T, Stodieck S, Huppertz HJ (2013) Comparison of morphometric analysis based on T1-and T2-weighted MRI data for visualization of focal cortical dysplasia. Epilepsy Res 106:403–409CrossRefPubMed
16.
Sone D, Sato N, Maikusa N, Ota M, Sumida K, Yokoyama K, Kimura Y, Imabayashi E, Watanabe Y, Watanabe M, Okazaki M, Onuma T, Matsuda H (2016) Automated subfield volumetric analysis of hippocampus in temporal lobe epilepsy using high-resolution T2-weighed MR imaging. Neuroimage Clin 12:57–64CrossRefPubMedPubMedCentral
17.
Wolf OT, Dyakin V, Patel A, Vadasz C, de Leon MJ, McEwen BS, Bulloch K (2002) Volumetric structural magnetic resonance imaging (MRI) of the rat hippocampus following kainic acid (KA) treatment. Brain Res 934:87–96CrossRefPubMed
18.
Bartnik-Olson BL, Ding D, Howe J, Shah A, Losey T (2017) Glutamate metabolism in temporal lobe epilepsy as revealed by dynamic proton MRS following the infusion of [U-13-C] glucose. Epilepsy Res 136:46–53CrossRefPubMed
19.
Xu MY, Ergene E, Zagardo M, Tracy PT, Wang H, Liu WC, Machens NA (2015) Proton MR spectroscopy in patients with structural MRI-negative temporal lobe epilepsy. J Neuroimaging 25:1030–1037CrossRefPubMed
20.
21.
Bessman SP, Carpenter CL (1985) The Creatine-Creatine phosphate energy shuttle. Annu Rev Biochem 54:831–862CrossRefPubMed
22.
Dulinska J, Setkowicz Z, Janeczko K, Sandt C, Dumas P, Uram L, Gzielo-Jurek K, Chwiej J (2012) Synchrotron radiation Fourier-transform infrared and Raman microspectroscopy study showing an increased frequency of creatine inclusions in the rat hippocampal formation following pilocarpine-induced seizures. Anal Bioanal Chem 402:2267–2274CrossRefPubMed
23.
Rambo LM, Ribeiro LR, Schramm VG, Berch AM, Stamm DN, Della-Pace ID, Silva LFA, Furian AF, Oliveira MS, Fighera MR, Royes LFF (2012) Creatine increases hippocampal Na(+), K(+)-ATPase activity via NMDA-calcineurin pathway. Brain Res Bull 88:553–559CrossRefPubMed
24.
Guivel-Scharen V, Sinnwell T, Wolff SD, Balaban RS (1998) Detection of proton chemical exchange between metabolites and water in biological tissues. J Magn Reson 133:36–45CrossRefPubMed
25.
Kogan F, Haris M, Debrosse C, Singh A, Nanga RP, Cai K, Hariharan H, Reddy R (2014) In vivo chemical exchange saturation transfer imaging of creatine (CrCEST) in skeletal muscle at 3T. J Magn Reson Imaging 40:596–602CrossRefPubMed
26.
Kogan F, Haris M, Singh A, Cai K, Debrosse C, Nanga RPR, Hariharan H, Reddy R (2014) Method for high-resolution imaging of creatine in vivo using chemical exchange saturation transfer. Magn Reson Med 71:164–172CrossRefPubMed
27.
Wolff SD, Balaban RS (1990) Nmr imaging of labile proton-exchange. J Magn Reson 86:164–169
28.
Hellier JL, Patrylo PR, Buckmaster PS, Dudek FE (1998) Recurrent spontaneous motor seizures after repeated low-dose systemic treatment with kainate: assessment of a rat model of temporal lobe epilepsy. Epilepsy Res 31:73–84CrossRefPubMed
29.
30.
Singh A, Cai KJ, Haris M, Hariharan H, Reddy R (2013) On B1 inhomogeneity correction of in vivo human brain glutamate chemical exchange saturation transfer contrast at 7T. Magn Reson Med 69:818–824CrossRefPubMed
31.
Tkac I, Starcuk Z, Choi IY, Gruetter R (1999) In vivo 1H NMR spectroscopy of rat brain at 1 ms echo time. Magn Reson Med 41:649–656CrossRef
32.
Paxinos GWC (2007) The rat brain in stereotaxic coordinates, 6th edn. Elsevier Academic, London
33.
Nasrallah FA, Pages G, Kuchel PW et al (2013) Imaging brain deoxyglucose uptake and metabolism by glucoCEST MRI. J Cereb Blood Flow Metabol 33:1270–1278CrossRef
34.
35.
Zhou J, Lal B, Wilson DA, Laterra J, van Zijl PCM (2003) Amide proton transfer (APT) contrast for imaging of brain tumors. Magn Reson Med 50:1120–1126CrossRefPubMed
36.
DeFrance JF, McCandless DW (1991) Energy metabolism in rat hippocampus during and following seizure activity. Metab Brain Dis 6:83–91CrossRefPubMed
37.
Petroff OA, Prichard JW, Behar KL et al (1984) In vivo phosphorus nuclear magnetic resonance spectroscopy in status epilepticus. Ann Neurol 16:169–177CrossRefPubMed
38.
Connelly A, Jackson GD, Duncan JS, King MD, Gadian DG (1994) Magnetic resonance spectroscopy in temporal lobe epilepsy. Neurology 44:1411–1417CrossRefPubMed
39.
Gadian DG, Connelly A, Duncan JS, Cross JH, Kirkham FJ, Johnson CL, Vargha-Khadem F, Nevile BG, Jackson GD (1994) 1H magnetic resonance spectroscopy in the investigation of intractable epilepsy. Acta Neurol Scand Suppl 152:116–121CrossRefPubMed
Metadata
Title
In Vivo Mapping and Quantification of Creatine Using Chemical Exchange Saturation Transfer Imaging in Rat Models of Epileptic Seizure
Authors
Dong-Hoon Lee
Do-Wan Lee
Jae-Im Kwon
Chul-Woong Woo
Sang-Tae Kim
Jin Seong Lee
Choong Gon Choi
Kyung Won Kim
Jeong Kon Kim
Dong-Cheol Woo
Publication date
01-04-2019
Publisher
Springer International Publishing
Published in
Molecular Imaging and Biology / Issue 2/2019
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI
https://doi.org/10.1007/s11307-018-1243-6

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