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Angiogenesis
Published in: Angiogenesis 4/2006

01-12-2006 | Original paper

Image analysis measurements of the microvascularisation in endometrium, superficial and deep endometriotic tissues

Authors: M. Jondet, M. C. Vacher-Lavenu, C. Chapron

Published in: Angiogenesis | Issue 4/2006

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Abstract

The aim of this study was to evaluate precisely the microvascularisation of endometrium, superficial and deep endometriotic lesions, in progestin-treated and non-treated patients suffering from endometriosis.
Methods: A population of 66 women was constituted. Immunohistochemistry was carried out with a specific marker of the endothelial cells (CD31). The number of vessels and the vessel area were assessed by a computer image analysis system.
Results: The number of vessels per mm2 were 211, 216, 225 and the vessel area was 270, 141 and 194 μm2, respectively in endometria, superficial and deep endometriotic lesions of untreated women. In endometria, superficial and deep endometriotic lesions of progestin-treated women the number of vessels were respectively 129, 149, and 181 per mm2 and the vessel area was 369, 474 and 254 μm2.
Conclusion: Statistically significant data indicate that endometriotic lesions are heterogeneous and suggest that progestin treatment induces a reduction in number and a concomitant dilation of microvessels with more microvascular changes in endometrium and superficial endometriotic lesions than in deep endometriotic lesions.
Literature
1.
Groothuis PG, Nap AW, Winterhager E, Grummer R (2005) Vascular development in endometriosis. Angiogenesis 8:147–156CrossRefPubMed
2.
Gargett C, Weston G, Rogers PAW (2002) Mechanism and regulation of endometrial Angiogenesis. Reprod Medec Rew 10:45–61CrossRef
3.
Donnez J, Smoes P, Gillerot S, Casanas-Roux F, Nisolle M (1998) Vascular endothelial growth factor (VEGF) in endometriosis. Hum Reprod 13:1686–1690CrossRefPubMed
4.
Mclaren J (2000) Vascular endothelial growth factor and endometriotic angiogenesis. Hum Reprod Update 6:45–55CrossRefPubMed
5.
Zygmunt M, Herr F, Keller-Schoenwetter S et al (2002) Characterization of human chorionic gonadotropin as a novel angiogenic factor. J Clin Endocrinol Metab 87:5290–96CrossRefPubMed
6.
Hayrabedyan S, Kyurkchiev S, Kehayov I (2005) Endoglin (cd105) and S100A13 as markers of active angiogenesis in endometriosis. Reprod Biol 5:51–67PubMed
7.
Girling JA, Rogers PAW (2005) Recent advances in endometrial angiogenesis research. Angiogenesis 8:89–99CrossRefPubMed
8.
Vercellini P, Fedele L, Pietropaolo G, Frontino G, Somigliana E, Crosignani PG (2003) Progestogens for endometriosis: forward to the past. Hum Reprod Update 4:387–396CrossRef
9.
Prentice A, Deary AJ, Bland E (2000) Progestagens and anti-progestagens for pain associated with endometriosis. Cochrane Database Syst Rev CD002122
10.
Rodriguez-Manzaneque JC, Graubert M, Iruela-Arispe ML (2000) Endothelial cell dysfunction following prolonged activation of progesterone receptor. Hum Reprod 15(suppl 3):39–47PubMed
11.
Jondet M, Dehennin L (2003) Impact of different progestins on endometrial different progestins on endometrial vascularisation of postmenopausal women. A retrospective image analysis morphometric study. Maturitas 46:165–171CrossRefPubMed
12.
Jondet M, Letellier B, Verdys MT (2005) Endometrial vascularization in levonorgestrel intrauterine devise users; computerized microvessel measurement study. Contraception 71:60–64CrossRefPubMed
13.
Nisolle M, Casana-Roux F, Anaf V, Mine J-M, Donnez J (1993) Morphometric study of the stromal vascularization in peritoneal endometriosis. Fertil Steril 59:681–684PubMed
14.
Matsuzaki S, Canis M, Murakami T, Dechelotte P, Bruhat MA, Okomura K (2001) Immunohistochemical analysis of the role of angiogenic status in the vasculature of peritoneal endometriosis. Fertil Steril 76:712–716CrossRefPubMed
15.
Chapron C, Fauconnier A, Vieira M et al (2003) Anatomical distribution of deeply infiltrating endometriosis: surgical implications and proposition for a classification. Hum Reprod 18:157–161CrossRefPubMed
16.
Turner HE, Harris AL, Melmed S, Wass JA (2003) Angiogenesis in endocrine tumors. Endocr Rev 24:600–632CrossRefPubMed
17.
Angulo J, Serra J (2003) Automatic analysis of DNA microarray images using mathematical morphology. Bioinformatics 19:553–562CrossRefPubMed
18.
Koninck PR, Kennedy SH, Barlow DH (1998) Endometriotic disease: the role of peritoneal fluid. Hum Reprod Update 4:741–751CrossRef
19.
Gazvani R, Templeton A (2002) Peritoneal environment, cytokines and angiogenesis in the pathophysiology of endometriosis. Reproduction 123:217–226CrossRefPubMed
20.
Healy DL, Rogers PAW, Hii L, Wingfield M (1998) Angiogenesis: a new theory for endometriosis. Hum Reprod Update 4:736–740CrossRefPubMed
21.
Rogers PAW, Au CLA, Affandi B (1993) Endometrial microsvasculature density during the normal menstrual cycle and following exposure to long-term levonorgestrel. Hum Reprod 8:1396–1404PubMed
22.
Bergqvist A, Ljungberg O, Myhre E (1984) Human endometrium and endometriotic tissue obtained simultaneously: a comparative histological study. Int J Gynecol Pathol 3:135–145PubMedCrossRef
23.
Chapron C, Boucher E, Fauconnier A, Vieira M, Dubuisson JB, Vacher-Lavenu MC (2002) Anatomopathological lesions of bladder endometriosis are heterogeneous. Fertil Steril 78:740–742CrossRefPubMed
24.
Bonte H, Chapron C, Vieira M et al (2002) Histologic appearance of endometriosis infiltrating uterosacral ligaments in women with painful symptoms. J Am Assoc Gynecol Laparosc 9:419–424CrossRef
25.
Heilier JF, Donnez O, Van Kerkhove V, Lison D, Donnez J (2006) Expression of aromatase (P450 aromatase/CYP19) in pritoneal and ovarian endometriotic tissues and deep endometriotic (adenomyotic) nodules of the rectovaginal septum. Fertil Steril 85:1516–1518CrossRefPubMed
26.
Uzan C, Cortez A, Dufournet C, Fauvet R, Siffroi JP, Darai E (2004) Eutopic endometrium and peritoneal, ovarian and bowel endometriotic tissues express a different profile of matrix metalloproteinases-2, -3 and -11, and of tissue inhibitor mettalloproteinases-1 and -2. Virchows Arch 445:603–609CrossRefPubMed
27.
Hull ML, Charnock-Jones DS, Chan CL et al (2003) Antiangiogenic agents are effective inhibitors of endometriosis. J Clin Endocrinol Metab 88:2889–2899CrossRefPubMed
28.
Nap AW, Griffioen AW, Dunselman GA et al (2004) Antiangiogenesis therapy for endometriosis. J Clin Endocrinol Metab 89:1089–1095CrossRefPubMed
29.
Brosens IA, Brosens JJ (2000) Redefining endometriosis: is deep endometriosis a progressive disease? Hum Reprod 15:1–3CrossRefPubMed
30.
Bulun SE, Cheng YH, Yin P et al (2006) Progesterone resistance in endomtriosis: link to failure to metabolize estradiol. Mol Cell Endocrinol 248:94–103CrossRefPubMed
31.
Anaf V, Simon P, El Nakadi I, Fayt I, Buxant F, Noel JC (2002) Hyperalgia, nerve infiltration and nerve growth factor expression in deep adenomyotic nodules, peritoneal and ovarian endometriosis. Hum Reprod 17:1895–1900CrossRefPubMed
32.
Chapron C, Fauconnier A, Dubuisson JB, Barakat H, Vieira M, Breart G (2003) Deep infiltrating endometriosis: relation between severity of dysmenorrhoea and extent of disease. Hum Reprod 18:760–766CrossRefPubMed
Metadata
Title
Image analysis measurements of the microvascularisation in endometrium, superficial and deep endometriotic tissues
Authors
M. Jondet
M. C. Vacher-Lavenu
C. Chapron
Publication date
01-12-2006
Publisher
Kluwer Academic Publishers
Published in
Angiogenesis / Issue 4/2006
Print ISSN: 0969-6970
Electronic ISSN: 1573-7209
DOI
https://doi.org/10.1007/s10456-006-9044-y

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