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01-03-2020 | Herpes Virus | Brief Report

Development of a cross-priming isothermal amplification assay based on the glycoprotein B gene for instant and rapid detection of feline herpesvirus type 1

Authors: Yuxin Tan, Guoying Dong, Hefeng Xu, Jiangting Niu, Wei Lu, Kai Wang, Hao Dong, Shuang Zhang, Hailong Huang, Guixue Hu

Published in: Archives of Virology | Issue 3/2020

Abstract

A cross-priming isothermal amplification (CPA) assay was developed for detection of feline herpesvirus type 1 (FHV-1). In this assay, the target fragment of the FHV-1 glycoprotein B gene is amplified rapidly by Bst DNA polymerase at a constant temperature (63 °C, 45 min), using a simple thermostat. The assay had no cross-reactions with four types of feline viruses, and the detection limit was 10⁰ copies/μl. The positive rate of clinical samples from CPA was 100% consistent with qPCR but higher than ordinary PCR, indicating its superiority to ordinary PCR. Visualization was achieved using SYBR Green I dye.

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Title: Development of a cross-priming isothermal amplification assay based on the glycoprotein B gene for instant and rapid detection of feline herpesvirus type 1
Authors: Yuxin Tan
Guoying Dong
Hefeng Xu
Jiangting Niu
Wei Lu
Kai Wang
Hao Dong
Shuang Zhang
Hailong Huang
Guixue Hu
Publication date: 01-03-2020
Publisher: Springer Vienna
Keyword: Herpes Virus
Published in: Archives of Virology / Issue 3/2020
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-020-04526-5

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