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International Journal of Implant Dentistry

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Open Access 01-12-2022 | Growth Factors | Research

Plasma rich in growth factors (PRGF) and leukocyte-platelet rich fibrin (L-PRF): comparative release of growth factors and biological effect on osteoblasts

Authors: Laura Baca-Gonzalez, Rebeca Serrano Zamora, Lisa Rancan, Francisco González Fernández-Tresguerres, Isabel Fernández-Tresguerres, Rosa M. López-Pintor, Juan López-Quiles, Isabel Leco, Jesús Torres

Published in: International Journal of Implant Dentistry | Issue 1/2022

Abstract

Purpose

To compare the release of platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF-I) and interleukin 1β (IL-1β) of plasma rich in growth factors (PRGF) and leucocyte platelet-rich fibrin (L-PRF) and to evaluate their biological implication in osteoblasts.

Methods

Blood from 3 healthy volunteers was processed into PRGF, immediate L-PRF (L-PRF 0ʹ) and L-PRF 30 min after collection (L-PRF-30ʹ) and a control group. Growth factors release were analyzed at 7 times by ELISA. Cell proliferation, collagen-I synthesis and alkaline phosphatase activity were assessed in primary cultures of human osteoblasts.

Results

A slower controlled release of IGF-I, VEGF and PDGF was observed in the PRGF group at day 14. A higher synthesis of type I collagen was also quantified in PRGF. L-PRF released significantly higher amounts of IL-1β, that was almost absent in the PRGF.

Conclusions

The addition of leukocytes dramatically increases the secretion of proinflammatory cytokines, which are likely to negatively influence the synthesis of type I collagen and alkaline phosphatase (ALP) by osteoblasts.

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Title: Plasma rich in growth factors (PRGF) and leukocyte-platelet rich fibrin (L-PRF): comparative release of growth factors and biological effect on osteoblasts
Authors: Laura Baca-Gonzalez
Rebeca Serrano Zamora
Lisa Rancan
Francisco González Fernández-Tresguerres
Isabel Fernández-Tresguerres
Rosa M. López-Pintor
Juan López-Quiles
Isabel Leco
Jesús Torres
Publication date: 01-12-2022
Publisher: Springer Berlin Heidelberg
Keywords: Growth Factors
Growth Factors
Published in: International Journal of Implant Dentistry / Issue 1/2022
Electronic ISSN: 2198-4034
DOI: https://doi.org/10.1186/s40729-022-00440-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Plasma rich in growth factors (PRGF) and leukocyte-platelet rich fibrin (L-PRF): comparative release of growth factors and biological effect on osteoblasts

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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