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BMC Gastroenterology
Published in: BMC Gastroenterology 1/2023

Open Access 01-12-2023 | Gastric Cancer | Research article

SMC1A facilitates gastric cancer cell proliferation, migration, and invasion via promoting SNAIL activated EMT

Authors: Yaling Liu, Xianrui Fang, Qianqian Wang, Da Xiao, Ting Zhou, Kuo Kang, Zhenyu Peng, Feng Ren, Jingyu Zhou

Published in: BMC Gastroenterology | Issue 1/2023

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Abstract

Background

Structural maintenance of chromosomes protein 1 A (SMC1A) is a crucial subunit of the cohesion protein complex and plays a vital role in cell cycle regulation, genomic stability maintenance, chromosome dynamics. Recent studies demonstrated that SMC1A participates in tumorigenesis. This reseach aims to explore the role and the underlying mechanisms of SMC1A in gastric cancer (GC).

Materials and methods

RT-qPCR and western blot were used to examine the expression levels of SMC1A in GC tissues and cell lines. The role of SMC1A on GC cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) were analyzed. Furthermore,the mechanism of SMC1A action was investigated.

Results

SMC1A was highly expressed in GC tissues and cell lines. The high expression of SMC1A indicated the poor overall survival of GC patients from Kaplan-Meier Plotter. Enhancing the expression of SMC1A in AGS cells remarkably promoted cell proliferation in vitro and in vivo, migration and invasion, Conversely, knockdown of SMC1A in HGC27 cells inhibited cell proliferation, migration and invasion. Moreover, it’s observed that SMC1A promoted EMT and malignant cell behaviors via regulating SNAIL.

Conclusion

Our study revealed that SMC1A promotes EMT process by upregulating SNAIL, which contributes to gastric cancer cell proliferation, migration and invasion. Therefore, targeting SMC1A may be a potential strategy to improve GC therapy.
Appendix
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Metadata
Title
SMC1A facilitates gastric cancer cell proliferation, migration, and invasion via promoting SNAIL activated EMT
Authors
Yaling Liu
Xianrui Fang
Qianqian Wang
Da Xiao
Ting Zhou
Kuo Kang
Zhenyu Peng
Feng Ren
Jingyu Zhou
Publication date
01-12-2023
Publisher
BioMed Central
Published in
BMC Gastroenterology / Issue 1/2023
Electronic ISSN: 1471-230X
DOI
https://doi.org/10.1186/s12876-023-02850-z

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