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Journal of Endocrinological Investigation
Published in: Journal of Endocrinological Investigation 9/2016

01-09-2016 | Original Article

Evidence for decreased expression of APPL1 associated with reduced insulin and adiponectin receptors expression in PCOS patients

Authors: R. Dehghan, M. Saidijam, M. Mehdizade, N. Shabab, M. Yavangi, T. Artimani

Published in: Journal of Endocrinological Investigation | Issue 9/2016

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Abstract

Purpose

To investigate the expression of Adaptor protein containing a PH domain, PTB domain and leucine zipper motif 1 (APPL1), insulin receptor (INSR), adiponectin and adiponectin receptors (adipoR1 and R2) and their possible associations in granulosa cells (GCs) of 22 polycystic ovary syndrome (PCOS) women compared to the 22 non-PCOS controls with normal ovulatory function matched for BMI (body mass index).

Methods

In this study, 44 infertile women aged 18-40 years undergoing in vitro fertilization (IVF) protocol were recruited. After follicular fluid collection, GCs were isolated and then purified with MACS (Micro Beads conjugated to monoclonal anti-human CD45 antibodies). RNA was extracted from GCs and quantitative real-time PCR (qRT-PCR) was performed to assess APPL1 gene expression.

Results

Expression of APPL1, insulin receptor and adiponectin system genes was significantly decreased in PCOS group compared to the controls.

Conclusions

Reduction of APPL1, insulin receptor and adiponectin system genes in GCs could be involved in the development of PCOS.
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Metadata
Title
Evidence for decreased expression of APPL1 associated with reduced insulin and adiponectin receptors expression in PCOS patients
Authors
R. Dehghan
M. Saidijam
M. Mehdizade
N. Shabab
M. Yavangi
T. Artimani
Publication date
01-09-2016
Publisher
Springer International Publishing
Published in
Journal of Endocrinological Investigation / Issue 9/2016
Electronic ISSN: 1720-8386
DOI
https://doi.org/10.1007/s40618-016-0468-y

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