Skip to main content
Top
Published in: European Archives of Oto-Rhino-Laryngology 4/2016

01-04-2016 | Otology

Evaluation of nasopharyngeal microbial flora and antibiogram and its relation to otitis media with effusion

Authors: Navid Nourizadeh, Kiarash Ghazvini, Vahideh Gharavi, Niloufar Nourizadeh, Rahman Movahed

Published in: European Archives of Oto-Rhino-Laryngology | Issue 4/2016

Login to get access

Abstract

Acute otitis media and otitis media with effusion (OME) are the main causes of hearing impairment in children which require proper treatment, mainly antibiotic therapy. Patients whom were appropriate candidates for adenoidectomy were divided into two groups regarding the presence of middle ear effusion. Adenoid tissue specimens were cultured in both groups and the bacterial flora and anti-microbial resistance pattern were determined. 72 patients were studied, 42 % had OME while 58 % did not. The following bacteria were isolated and cultured from both groups with no meaningful difference in prevalence: Streptococcus viridans (p = 0.265), Staphylococcus aureus (p = 0.72), H. influenza (p = 0.806), Entrococcus. spp (0.391), Streptococcus pneumonia (p = 0.391), nonhemolytic Streptococcus (p = 0.230). Bacterial sensitivity was similar for Amoxicillin–clavulanate (p = 0.935), Amoxicillin (p = 0.935), Cephalexin (p = 0.806), Cefixime (p = 0.391) and Azithromycin in both groups. The two groups showed no meaningful difference considering the bacterial flora of nasopharynx and their sensitivity. Bacteria in both groups were sensitive to Amoxicillin and Amoxicillin–clavulanate and resistant to Azithromycin, Cefixime and Cephalexin.
Literature
1.
go back to reference Boston M, McCook J, Burke B, Derkay C (2003) Incidence of and risk factors for additional tympanostomy tube insertion in children. Arch Otolaryngol Head Neck Surg 129(3):293–296CrossRefPubMed Boston M, McCook J, Burke B, Derkay C (2003) Incidence of and risk factors for additional tympanostomy tube insertion in children. Arch Otolaryngol Head Neck Surg 129(3):293–296CrossRefPubMed
2.
go back to reference Inglis AF, Gates GA (2005) Acute otitis media and otitismedia with effusion. In: Cummings CW, Flint PW, Harlier LA, et al. Cummings Otolaryngology-Head and Neck Surgery. 4th ed. Philadelphia: Mosby. Vol 4, pp 4446–7 Inglis AF, Gates GA (2005) Acute otitis media and otitismedia with effusion. In: Cummings CW, Flint PW, Harlier LA, et al. Cummings Otolaryngology-Head and Neck Surgery. 4th ed. Philadelphia: Mosby. Vol 4, pp 4446–7
3.
go back to reference Flint PW, Haughey BH, Lund VJ, Niparko JK, Richardson MA, Robbins K, et al (2013) Cummings otolaryngology head and neck surgery. 15th ed. Philadelphia: Mosby Flint PW, Haughey BH, Lund VJ, Niparko JK, Richardson MA, Robbins K, et al (2013) Cummings otolaryngology head and neck surgery. 15th ed. Philadelphia: Mosby
4.
go back to reference Hall-Stoodley L, Hu FZ, Gieseke A, Nistico L, Nguyen D, Hayes J et al (2006) Direct detection of bacterial biofilms on the middle-ear mucosa of children with chronic otitis media. JAMA 296(2):202–211CrossRefPubMedPubMedCentral Hall-Stoodley L, Hu FZ, Gieseke A, Nistico L, Nguyen D, Hayes J et al (2006) Direct detection of bacterial biofilms on the middle-ear mucosa of children with chronic otitis media. JAMA 296(2):202–211CrossRefPubMedPubMedCentral
6.
go back to reference Seiden AM, Tami TA, Pensak ML, Cotton RT, Glucman JL (2001) Otolaryngology the essentials. 13th ed. USA: Thieme Seiden AM, Tami TA, Pensak ML, Cotton RT, Glucman JL (2001) Otolaryngology the essentials. 13th ed. USA: Thieme
7.
go back to reference Kubba H, Pearson JP, Birchall JP (2000) The aetiology of otitis media with effusion: a review. Clin Otolaryngol Allied Sci 25(3):181–194CrossRefPubMed Kubba H, Pearson JP, Birchall JP (2000) The aetiology of otitis media with effusion: a review. Clin Otolaryngol Allied Sci 25(3):181–194CrossRefPubMed
8.
go back to reference Ehrlich GD, Veeh R, Wang X et al (2002) Mucosal biofilm formation on middle-ear mucosa in the chinchilla model of otitis media. JAMA 287(13):1710–1715CrossRefPubMed Ehrlich GD, Veeh R, Wang X et al (2002) Mucosal biofilm formation on middle-ear mucosa in the chinchilla model of otitis media. JAMA 287(13):1710–1715CrossRefPubMed
9.
go back to reference Hall-Stoodley L, Hu FZ, Gieseke A et al (2006) Direct detection of bacterial biofilms on the middle-ear mucosa of children with chronic otitis media. JAMA 296(2):202–211CrossRefPubMedPubMedCentral Hall-Stoodley L, Hu FZ, Gieseke A et al (2006) Direct detection of bacterial biofilms on the middle-ear mucosa of children with chronic otitis media. JAMA 296(2):202–211CrossRefPubMedPubMedCentral
10.
go back to reference Gok U, Bulut Y, Keles E, Yalcin S, Doymaz MZ (2001) Bacteriological and PCR analysis of clinical material aspirated from otitis media with effusions. Int J Pediatr Otorhinolaryngol 60(1):49–54CrossRefPubMed Gok U, Bulut Y, Keles E, Yalcin S, Doymaz MZ (2001) Bacteriological and PCR analysis of clinical material aspirated from otitis media with effusions. Int J Pediatr Otorhinolaryngol 60(1):49–54CrossRefPubMed
11.
go back to reference Miura MS, Mascaro M, Rosenfeld RM (2012) Association between otitis media and gastroesophageal reflux: a systematic review. Otolaryngol Head Neck Surg 146(3):345–352CrossRefPubMed Miura MS, Mascaro M, Rosenfeld RM (2012) Association between otitis media and gastroesophageal reflux: a systematic review. Otolaryngol Head Neck Surg 146(3):345–352CrossRefPubMed
12.
go back to reference Dodson KM, Cohen RS, Rubin BK (2012) Middle ear fluid characteristics in pediatric otitis media with effusion. Int J Pediatr Otorhinolaryngol 76(12):1806–1809CrossRefPubMed Dodson KM, Cohen RS, Rubin BK (2012) Middle ear fluid characteristics in pediatric otitis media with effusion. Int J Pediatr Otorhinolaryngol 76(12):1806–1809CrossRefPubMed
13.
go back to reference Inagaki M, Sakakura Y, Shimizu T, Majima Y, Ukai K (1988) Ultrastructure of mucous blanket in otitis media with effusion. Ann Otol Rhinol Laryngol 97(3 Pt 1):313–317CrossRefPubMed Inagaki M, Sakakura Y, Shimizu T, Majima Y, Ukai K (1988) Ultrastructure of mucous blanket in otitis media with effusion. Ann Otol Rhinol Laryngol 97(3 Pt 1):313–317CrossRefPubMed
14.
go back to reference Alles R, Parikh A, Hawk L, Darby Y, Romero JN, Scadding G (2001) The prevalence of atopic disorders in children with chronic otitis media with effusion. Pediatr Allergy Immunol 12(2):102–106CrossRefPubMed Alles R, Parikh A, Hawk L, Darby Y, Romero JN, Scadding G (2001) The prevalence of atopic disorders in children with chronic otitis media with effusion. Pediatr Allergy Immunol 12(2):102–106CrossRefPubMed
15.
go back to reference Hurst DS, Venge P (2002) The impact of atopy on neutrophil activity in middle ear effusion from children and adults with chronic otitis media. Arch Otolaryngol Head Neck Surg 128(5):561–566CrossRefPubMed Hurst DS, Venge P (2002) The impact of atopy on neutrophil activity in middle ear effusion from children and adults with chronic otitis media. Arch Otolaryngol Head Neck Surg 128(5):561–566CrossRefPubMed
16.
go back to reference Kreiner-Møller E, Chawes BL, Caye-Thomasen P, Bønnelykke K, Bisgaard H (2012) Allergic rhinitis is associated with otitis media with effusion: a birth cohort study. Clin Exp Allergy 42(11):1615–1620CrossRefPubMed Kreiner-Møller E, Chawes BL, Caye-Thomasen P, Bønnelykke K, Bisgaard H (2012) Allergic rhinitis is associated with otitis media with effusion: a birth cohort study. Clin Exp Allergy 42(11):1615–1620CrossRefPubMed
17.
go back to reference Martines F, Bentivegna D, Maira E, Sciacca V, Martines E (2011) Risk factors for otitis media with effusion: case-control study in Sicilian school children. Int J Pediatr Otorhinolaryngol 75(6):754–759CrossRefPubMed Martines F, Bentivegna D, Maira E, Sciacca V, Martines E (2011) Risk factors for otitis media with effusion: case-control study in Sicilian school children. Int J Pediatr Otorhinolaryngol 75(6):754–759CrossRefPubMed
18.
go back to reference Rye MS, Warrington NM, Scaman ES et al (2012) Genome-wide association study to identify the genetic determinants of otitis media susceptibility in childhood. PLoS One 7(10):e48215CrossRefPubMedPubMedCentral Rye MS, Warrington NM, Scaman ES et al (2012) Genome-wide association study to identify the genetic determinants of otitis media susceptibility in childhood. PLoS One 7(10):e48215CrossRefPubMedPubMedCentral
19.
go back to reference Hafrén L, Kentala E, Järvinen TM, Leinonen E, Onkamo P, Kere J, Mattila PS (2012) Genetic background and the risk of otitis media. Int J Pediatr Otorhinolaryngol 76(1):41–44CrossRefPubMed Hafrén L, Kentala E, Järvinen TM, Leinonen E, Onkamo P, Kere J, Mattila PS (2012) Genetic background and the risk of otitis media. Int J Pediatr Otorhinolaryngol 76(1):41–44CrossRefPubMed
20.
go back to reference Bluestone CD, Stephenson JS, Martin LM (1992) Ten-year review of otitis media pathogens. Pediatr Infect Dis J 11(8 Suppl):S7–S11CrossRefPubMed Bluestone CD, Stephenson JS, Martin LM (1992) Ten-year review of otitis media pathogens. Pediatr Infect Dis J 11(8 Suppl):S7–S11CrossRefPubMed
21.
go back to reference Poetker DM, Lindstrom DR, Edmiston CE, Krepel CJ, Link TR, Kerschner JE (2005) Microbiology of middle ear effusions from 292 patients undergoing tympanostomy tube placement for middle ear disease. Int J Pediatr Otorhinolaryngol 69(6):799–804CrossRefPubMed Poetker DM, Lindstrom DR, Edmiston CE, Krepel CJ, Link TR, Kerschner JE (2005) Microbiology of middle ear effusions from 292 patients undergoing tympanostomy tube placement for middle ear disease. Int J Pediatr Otorhinolaryngol 69(6):799–804CrossRefPubMed
22.
go back to reference Brook I, Yocum P, Shah K, Feldman B, Epstein S (2001) Microbiology of serous otitis media in children: correlation with age and length of effusion. Ann Otol Rhinol Laryngol 110(1):87–90CrossRefPubMed Brook I, Yocum P, Shah K, Feldman B, Epstein S (2001) Microbiology of serous otitis media in children: correlation with age and length of effusion. Ann Otol Rhinol Laryngol 110(1):87–90CrossRefPubMed
23.
go back to reference Post JC, Preston RA, Aul JJ et al (1995) Molecular analysis of bacterial pathogens in otitis media with effusion. JAMA 273(20):1598–1604CrossRefPubMed Post JC, Preston RA, Aul JJ et al (1995) Molecular analysis of bacterial pathogens in otitis media with effusion. JAMA 273(20):1598–1604CrossRefPubMed
24.
go back to reference Rayner MG, Zhang Y, Gorry MC, Chen Y, Post JC, Ehrlich GD (1998) Evidence of bacterial metabolic activity in culture-negative otitis media with effusion. JAMA 279(4):296–299CrossRefPubMed Rayner MG, Zhang Y, Gorry MC, Chen Y, Post JC, Ehrlich GD (1998) Evidence of bacterial metabolic activity in culture-negative otitis media with effusion. JAMA 279(4):296–299CrossRefPubMed
25.
go back to reference Mc Clay JE (2000) Resistant bacteria in the adenoid. Arch Otolaryngol Head Neck Surg 126(5):625–629CrossRef Mc Clay JE (2000) Resistant bacteria in the adenoid. Arch Otolaryngol Head Neck Surg 126(5):625–629CrossRef
26.
go back to reference Marchisio P, Claut L, Rognoni A, Esposito S, Passali D, Bellussi L et al (2003) Differences in nasopharyngeal bacterial flora in children with nonsevere recurrent acute otitis media and chronic otitis media with effusion: implications for management. Pediatr Infect Dis J 22(3):262–268PubMed Marchisio P, Claut L, Rognoni A, Esposito S, Passali D, Bellussi L et al (2003) Differences in nasopharyngeal bacterial flora in children with nonsevere recurrent acute otitis media and chronic otitis media with effusion: implications for management. Pediatr Infect Dis J 22(3):262–268PubMed
27.
go back to reference Almac A, Elicora SS, Yumuk Z, Dundar V, Willke A (2009) The relationship between chronic otitis media with effusion and surface and deep flora of hypertrophic adenoids. Int J Pediatr Otorhinolaryngol 73:1438–1440CrossRefPubMed Almac A, Elicora SS, Yumuk Z, Dundar V, Willke A (2009) The relationship between chronic otitis media with effusion and surface and deep flora of hypertrophic adenoids. Int J Pediatr Otorhinolaryngol 73:1438–1440CrossRefPubMed
28.
go back to reference Linder TE, Marder HP, Munzinger J (1997) Role of adenoids in the pathogenesis of otitis media: a bacteriologic and immunohistochemical analysis. Ann Otol Rhinol Laryngol 106(8):619–623CrossRefPubMed Linder TE, Marder HP, Munzinger J (1997) Role of adenoids in the pathogenesis of otitis media: a bacteriologic and immunohistochemical analysis. Ann Otol Rhinol Laryngol 106(8):619–623CrossRefPubMed
29.
go back to reference Saafan ME, Ibrahim WS, Tomoum MO (2013) Role of adenoid biofilm in chronic otitis media with effusion in children. Eur Arch Otorhinolaryngol 270(9):2417–2425CrossRefPubMed Saafan ME, Ibrahim WS, Tomoum MO (2013) Role of adenoid biofilm in chronic otitis media with effusion in children. Eur Arch Otorhinolaryngol 270(9):2417–2425CrossRefPubMed
30.
go back to reference Tos M (1984) Epidemiology and natural history of secretory otitis. Am J Otol 5:459PubMed Tos M (1984) Epidemiology and natural history of secretory otitis. Am J Otol 5:459PubMed
31.
go back to reference Williamson IG, Dunleavey J, Bain J, Robinson D (1994) The natural history of otitis media with effusion—a 3-year study of the incidence and prevalence of abnormal tympanograms in four South West Hampshire infant and first schools. J Laryngol Otol 108:930CrossRefPubMed Williamson IG, Dunleavey J, Bain J, Robinson D (1994) The natural history of otitis media with effusion—a 3-year study of the incidence and prevalence of abnormal tympanograms in four South West Hampshire infant and first schools. J Laryngol Otol 108:930CrossRefPubMed
32.
33.
go back to reference Teele DW, Klein JO, Rosner BA (1980) Epidemiology of otitis media in children. Ann Otol Rhinol Laryngol Suppl 89:5PubMed Teele DW, Klein JO, Rosner BA (1980) Epidemiology of otitis media in children. Ann Otol Rhinol Laryngol Suppl 89:5PubMed
34.
go back to reference van Zon A, van der Heijden GJ, van Dongen TM et al (2012) Antibiotics for otitis media with effusion in children. Cochrane Database Syst Rev 9:63 van Zon A, van der Heijden GJ, van Dongen TM et al (2012) Antibiotics for otitis media with effusion in children. Cochrane Database Syst Rev 9:63
35.
go back to reference Rosenfeld RM, Post JC (1992) Meta-analysis of antibiotics for the treatment of otitis media with effusion. Otolaryngol Head Neck Surg 106:378PubMed Rosenfeld RM, Post JC (1992) Meta-analysis of antibiotics for the treatment of otitis media with effusion. Otolaryngol Head Neck Surg 106:378PubMed
36.
go back to reference Williams RL, Chalmers TC, Stange KC et al (1993) Use of antibiotics in preventing recurrent acute otitis media and in treating otitis media with effusion. A meta-analytic attempt to resolve the brouhaha. JAMA 270:1344CrossRefPubMed Williams RL, Chalmers TC, Stange KC et al (1993) Use of antibiotics in preventing recurrent acute otitis media and in treating otitis media with effusion. A meta-analytic attempt to resolve the brouhaha. JAMA 270:1344CrossRefPubMed
37.
go back to reference American Academy of Family Physicians, American academy of otolaryngology-head and neck surgery, American academy of pediatrics subcommittee on otitis media with effusion (2004) Otitis media with effusion. Pediatrics 113:1412CrossRef American Academy of Family Physicians, American academy of otolaryngology-head and neck surgery, American academy of pediatrics subcommittee on otitis media with effusion (2004) Otitis media with effusion. Pediatrics 113:1412CrossRef
38.
go back to reference Khoramrooz SS, Mirsalehian A, Emaneini M, Jabalameli F, Aligholi M, Saedi B et al (2012) Frequency of Alloicoccus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in children with otitis media with effusion (OME) in Iranian patients. Auris Nasus Larynx 39(4):369–373CrossRefPubMed Khoramrooz SS, Mirsalehian A, Emaneini M, Jabalameli F, Aligholi M, Saedi B et al (2012) Frequency of Alloicoccus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in children with otitis media with effusion (OME) in Iranian patients. Auris Nasus Larynx 39(4):369–373CrossRefPubMed
Metadata
Title
Evaluation of nasopharyngeal microbial flora and antibiogram and its relation to otitis media with effusion
Authors
Navid Nourizadeh
Kiarash Ghazvini
Vahideh Gharavi
Niloufar Nourizadeh
Rahman Movahed
Publication date
01-04-2016
Publisher
Springer Berlin Heidelberg
Published in
European Archives of Oto-Rhino-Laryngology / Issue 4/2016
Print ISSN: 0937-4477
Electronic ISSN: 1434-4726
DOI
https://doi.org/10.1007/s00405-015-3637-2

Other articles of this Issue 4/2016

European Archives of Oto-Rhino-Laryngology 4/2016 Go to the issue