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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 5/2020

01-05-2020 | Endometriosis | Reproductive Physiology and Disease

Spatial and temporal changes in the expression of steroid hormone receptors in mouse model of endometriosis

Authors: Anuradha Mishra, Mosami Galvankar, Neha Singh, Deepak Modi

Published in: Journal of Assisted Reproduction and Genetics | Issue 5/2020

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Abstract

Purpose

Endometriosis is recognized as a steroid hormone-dependent disorder. However, controversies exist regarding the status of the steroid hormone receptor expression in endometriotic tissues. The purpose of this study was to determine the ontogeny of cellular changes in the expression of estrogen receptors (ERα, ERβ), G protein-coupled estrogen receptor 1 (GPER1), and progesterone receptors (PRs) in endometriosis using a mouse model.

Methods

We used the autologous uterine tissue transfer mouse model and studied the mRNA and protein expression of ERα, ERβ, GPER1, and PR in ectopic lesions at 2, 4, and 8 weeks of induction of endometriosis.

Result

As compared to endometrium of controls, in the ectopic endometrium, ERα is reduced while ERβ was elevated in stromal cells; however, Gper1 and PR levels are reduced in both stromal and epithelial cells in a time-specific manner. There is a high inter-animal variation in the levels of these receptors in ectopic endometrium as compared to controls; the levels also varied by almost 100-fold within the same lesion resulting in “micro-heterogeneity.” The expression of all these receptors also deferred between two lesions from the same animal.

Conclusion

In the endometriotic tissue, there is extensive inter-animal and intra-lesion heterogeneity in the expression of ERα, ERβ, GPER1, and PR. These changes are not due to the influence of the peritoneal environment but appear to be tissue intrinsic. We propose that the variable outcomes in hormonal therapy for endometriosis could be possibly due to heterogeneity in the expression of steroid hormone receptors in the ectopic endometrium.
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Metadata
Title
Spatial and temporal changes in the expression of steroid hormone receptors in mouse model of endometriosis
Authors
Anuradha Mishra
Mosami Galvankar
Neha Singh
Deepak Modi
Publication date
01-05-2020
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 5/2020
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-020-01725-6

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