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Lasers in Medical Science
Published in: Lasers in Medical Science 5/2014

01-09-2014 | Original Article

Effects of laser irradiation (670-nm InGaP and 830-nm GaAlAs) on burn of second-degree in rats

Authors: Gabriela Bortolança Chiarotto, Lia Mara Grosso Neves, Marcelo Augusto Marreto Esquisatto, Maria Esméria Corezola do Amaral, Gláucia Maria Tech dos Santos, Fernanda Aparecida Sampaio Mendonça

Published in: Lasers in Medical Science | Issue 5/2014

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Abstract

This study investigated the effects of 670-nm indium gallium phosphide (InGaP) and 830-nm gallium aluminum arsenide (GaAlAs) laser therapy on second-degree burns induced on the back of Wistar rats. Sixty-three male Wistar rats were anesthetized, and second-degree burns were made on their back. The animals were then divided randomly into three groups: control (C), animals treated with 670-nm InGaP laser (LIn), and animals treated with 830-nm GaAlAs laser (LGa). The wound areas were removed after 2, 6, 10, 14, and 18 days of treatment and submitted to structural and morphometric analysis. The following parameters were studied: total number of granulocytes and fibroblasts, number of newly formed blood vessels, and percentage of birefringent collagen fibers in the repair area. Morphometric analysis showed that different lasers 670-nm InGaP and 830-nm GaAlAs reduced the number of granulocytes and an increase of newly formed vessels in radiated lesions. The 670-nm InGaP laser therapy was more effective in increasing the number of fibroblasts. The different treatments modified the expression of VEGF and TGF-β1, when compared with lesions not irradiated. The different types of light sources showed similar effects, improved the healing of second-degree burns and can help for treating this type of injury. Despite the large number of studies with LLTI application in second-degree burns, there is still divergence about the best irradiation parameters to be used. Further studies are needed for developing a protocol effective in treating this type of injury.
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Metadata
Title
Effects of laser irradiation (670-nm InGaP and 830-nm GaAlAs) on burn of second-degree in rats
Authors
Gabriela Bortolança Chiarotto
Lia Mara Grosso Neves
Marcelo Augusto Marreto Esquisatto
Maria Esméria Corezola do Amaral
Gláucia Maria Tech dos Santos
Fernanda Aparecida Sampaio Mendonça
Publication date
01-09-2014
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 5/2014
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-014-1573-9

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